RNH1 RNA-DNA hybrid ribonuclease [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RNH1
Official Full Name: RNA-DNA hybrid ribonuclease
Primary source: SGD:S000004847
Locus tag: YMR234W
See related: AllianceGenome:SGD:S000004847; FungiDB:YMR234W; VEuPathDB:YMR234W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA-DNA hybrid ribonuclease activity. Involved in RNA catabolic process. Located in cytoplasm and nucleus. Human ortholog(s) of this gene implicated in autosomal recessive progressive external ophthalmoplegia with mitochondrial DNA deletions 2. Orthologous to human RNASEH1 (ribonuclease H1). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See RNH1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (740266..741312)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Here we show that the translesion synthesis DNA polymerase eta (Pol eta) plays a role in DNA replication under low deoxyribonucleotides condition triggered by hydroxyurea. In particular, the catalytic reaction performed by Pol eta is detrimental for RNase H deficient cells, causing DNA damage checkpoint activation and G2/M arrest
Title: RNase H activities counteract a toxic effect of Polymerase η in cells replicating with depleted dNTP pools.
we observed a similar increase in repeat instability in RNase H-deficient cells when we either changed the direction of transcription-replication collisions, or flipped the repeat sequence such that the (UUC)n run occurred in the transcript. The increase in repeat expansions in RNase H-deficient strains was dependent on Rad52 and Pol32 proteins, suggesting that break-induced replication (BIR) is responsible for this effe
Title: RNA-DNA hybrids promote the expansion of Friedreich's ataxia (GAA)n repeats via break-induced replication.
Depleting endogenous RNase H activity impairs R-loop removal in Saccharomyces cerevisiae, causing DNA damage that occurs preferentially in the repetitive ribosomal DNA locus (rDNA).
Title: RNase H enables efficient repair of R-loop induced DNA damage.
high-resolution mapping of mitotic recombination events throughout the yeast genome in diploid strains of Saccharomyces cerevisiae lacking RNase H1 (rnh1Delta), RNase H2 (rnh201Delta), or both RNase H1 and RNase H2 (rnh1Delta rnh201Delta)
Title: Elevated Genome-Wide Instability in Yeast Mutants Lacking RNase H Activity.
Data show that overexpression of a cellular RNase H, which degrades RNA in an RNA:DNA hybrid, completely suppressed the increase in Ty1 multimer formation in an rrm3 mutant.
Title: Rrm3 protects the Saccharomyces cerevisiae genome from instability at nascent sites of retrotransposition.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA-DNA hybrid ribonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA-DNA hybrid ribonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA-DNA hybrid ribonuclease activity	IEA Inferred from Electronic Annotation more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA replication, removal of RNA primer	IBA Inferred from Biological aspect of Ancestor more info
involved_in RNA catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed

General protein information

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Preferred Names: RNA-DNA hybrid ribonuclease

NP_013961.1

EC 3.1.26.4

Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I.

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

740266..741312

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182741.1 → NP_013961.1 TPA: RNA-DNA hybrid ribonuclease [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013961.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W060, Q04740

UniProtKB/TrEMBL

A6ZMU7, B3LMC3, B5VQ02, C8ZF99, G2WKU2, N1NXM4

Conserved Domains (2) summary

COG3341
Location:1 → 180

Rnh1; RNase HI-related protein, contains viroplasmin and RNaseH domains [General function prediction only]

cd09280
Location:190 → 345

RNase_HI_eukaryote_like; Eukaryotic RNase H is essential and is longer and more complex than their prokaryotic counterparts