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ASI1 putative ubiquitin-protein ligase ASI1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 855147, updated on 18-Sep-2024

Summary

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Official Symbol
ASI1
Official Full Name
putative ubiquitin-protein ligase ASI1
Primary source
SGD:S000004725
Locus tag
YMR119W
See related
AllianceGenome:SGD:S000004725; FungiDB:YMR119W; VEuPathDB:YMR119W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables ubiquitin-protein transferase activity. Involved in cellular response to amino acid stimulus and transcription factor catabolic process. Located in nuclear inner membrane. Part of Asi complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ASI1 in Genome Data Viewer
Location:
chromosome: XIII
Exon count:
1
Sequence:
Chromosome: XIII; NC_001145.3 (505333..507207)

Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene protein SHH3 Neighboring gene tRNA-Leu Neighboring gene bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE17 Neighboring gene ribosomal 60S subunit protein L15B

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae. Woodruff KA, et al. MicroPubl Biol, 2021 Jun 1. PMID 34095778, Free PMC Article
  2. Mapping genetic variants underlying differences in the central nitrogen metabolism in fermenter yeasts. Jara M, et al. PLoS One, 2014. PMID 24466135, Free PMC Article
  3. Asi1 is an inner nuclear membrane protein that restricts promoter access of two latent transcription factors. Boban M, et al. J Cell Biol, 2006 Jun 5. PMID 16735580, Free PMC Article
  4. The N-terminal regulatory domain of Stp1p is modular and, fused to an artificial transcription factor, confers full Ssy1p-Ptr3p-Ssy5p sensor control. Andréasson C, et al. Mol Cell Biol, 2004 Sep. PMID 15314160, Free PMC Article
  5. Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2. Zargari A, et al. J Biol Chem, 2007 Jan 5. PMID 17085444

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Asi1 regulates distribution of proteins at the nuclear membrane.
    Title: Distribution of Proteins at the Inner Nuclear Membrane Is Regulated by the Asi1 E3 Ligase in Saccharomyces cerevisiae.
  2. In dal81Delta mutants, the repressing activity of the Asi proteins is dispensable, demonstrating that without amplification, the levels of full-length Stp1 and Stp2 that escape cytoplasmic retention are insufficient to activate transcription.
    Title: Dal81 enhances Stp1- and Stp2-dependent transcription necessitating negative modulation by inner nuclear membrane protein Asi1 in Saccharomyces cerevisiae.
  3. Latent precursor forms of Stp1 and Stp2 inefficiently enter the nucleus where Asi1 restricts them from binding SPS sensor-regulated promoters, revealing an unanticipated role of inner nuclear membrane proteins in controlling gene expression
    Title: Asi1 is an inner nuclear membrane protein that restricts promoter access of two latent transcription factors.
  4. Asi1, Asi2, and Asi3 work together to maintain the latent properties of transcription factors Stp1 and Stp2
    Title: Inner nuclear membrane proteins Asi1, Asi2, and Asi3 function in concert to maintain the latent properties of transcription factors Stp1 and Stp2.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin-protein transferase activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables ubiquitin-protein transferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in cellular response to amino acid stimulus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ubiquitin-dependent protein catabolic process IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of Asi complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in nuclear inner membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear inner membrane IEA
Inferred from Electronic Annotation
more info
  
located_in nuclear periphery HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
putative ubiquitin-protein ligase ASI1
NP_013837.1
  • Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001145.3 Reference assembly

    Range
    505333..507207
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182619.1 → NP_013837.1  TPA: putative ubiquitin-protein ligase ASI1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013837.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VZU2, P54074
    UniProtKB/TrEMBL
    C7GU99, C8ZEX2, G2WKG9, N1NYT3
    Conserved Domains (1) summary
    cd16616
    Location:568 → 611
    mRING-HC-C4C4_Asi1p_like; Modified RING finger, HC subclass (C4C4-type), found in Saccharomyces cerevisiae amino acid sensor-independent protein Asi1p, Asi3p and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P54074.1

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