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CGI121 Cgi121p [ Saccharomyces cerevisiae S288C ]

Gene ID: 854972, updated on 3-Nov-2024

Summary

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Official Symbol
CGI121
Official Full Name
Cgi121p
Primary source
SGD:S000004500
Locus tag
YML036W
See related
AllianceGenome:SGD:S000004500; FungiDB:YML036W; VEuPathDB:YML036W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables tRNA binding activity. Involved in positive regulation of transcription by RNA polymerase II and telomere maintenance via recombination. Part of EKC/KEOPS complex. Human ortholog(s) of this gene implicated in Galloway-Mowat syndrome 5. Orthologous to human TPRKB (TP53RK binding protein). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See CGI121 in Genome Data Viewer
Location:
chromosome: XIII
Exon count:
2
Sequence:
Chromosome: XIII; NC_001145.3 (205642..206293)

Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene Ymd8p Neighboring gene Lft1p Neighboring gene AMP deaminase Neighboring gene Src1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A substrate binding model for the KEOPS tRNA modifying complex. Beenstock J, et al. Nat Commun, 2020 Dec 4. PMID 33277478, Free PMC Article
  2. In vitro biosynthesis of a universal t6A tRNA modification in Archaea and Eukarya. Perrochia L, et al. Nucleic Acids Res, 2013 Feb 1. PMID 23258706, Free PMC Article
  3. Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex. Zhang W, et al. Nucleic Acids Res, 2015 Mar 31. PMID 25735745, Free PMC Article
  4. Structural and functional characterization of KEOPS dimerization by Pcc1 and its role in t6A biosynthesis. Wan LC, et al. Nucleic Acids Res, 2016 Aug 19. PMID 27302132, Free PMC Article
  5. Reconstitution and characterization of eukaryotic N6-threonylcarbamoylation of tRNA using a minimal enzyme system. Wan LC, et al. Nucleic Acids Res, 2013 Jul. PMID 23620299, Free PMC Article

See all (47) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Reciprocal hemizygosity analysis reveals that the Saccharomyces cerevisiae CGI121 gene affects lag time duration in synthetic grape must.
    Title: Reciprocal hemizygosity analysis reveals that the Saccharomyces cerevisiae CGI121 gene affects lag time duration in synthetic grape must.
  2. Deletion of CGI121 in the short-lived yku80(tel) mutant restores lifespan to cgi121Delta level, supporting the function of Cgi121 in telomeric single-stranded DNA generation and thus in promotion of telomere recombination.
    Title: Inhibition of telomere recombination by inactivation of KEOPS subunit Cgi121 promotes cell longevity.
  3. the crystal structure of Bud32/Cgi121 in complex with ADP revealed that ADP is bound in the catalytic site of Bud32 in a canonical manner
    Title: Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex.
  4. EKC/KEOPS complex is specifically involved in the regulation of Bud9p localization downstream of Rax1p/Rax2p.
    Title: Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC/KEOPS complex.
  5. Cgi121 is part of the conserved KEOPS protein complex which promotes both telomere uncapping and telomere elongation
    Title: A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables tRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA threonylcarbamoyladenosine modification IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in telomere maintenance IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in telomere maintenance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in telomere maintenance via recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in telomere maintenance via recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of EKC/KEOPS complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of EKC/KEOPS complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in chromosome, telomeric region IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Cgi121p
NP_013676.2
  • Component of the EKC/KEOPS complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; Cgi121p is dispensable for tRNA modification; other complex members are Bud32p, Kae1p, Pcc1p, and Gon7p; ortholog of human TPRKB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001145.3 Reference assembly

    Range
    205642..206293
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182394.1 → NP_013676.2  TPA: Cgi121p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013676.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A2TBM6, D6VZD9, Q03705, Q6Q5A2
    UniProtKB/TrEMBL
    C7GT65, C8ZEG9
    Conserved Domains (1) summary
    pfam08617
    Location:19 → 179
    CGI-121; Kinase binding protein CGI-121
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q03705.1

Gene LinkOut

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