PHO84 phosphate transporter PHO84 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PHO84
Official Full Name: phosphate transporter PHO84
Primary source: SGD:S000004592
Locus tag: YML123C
See related: AllianceGenome:SGD:S000004592; FungiDB:YML123C; VEuPathDB:YML123C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables manganese ion transmembrane transporter activity; phosphate transmembrane transporter activity; and selenite:proton symporter activity. Involved in several processes, including manganese ion transport; plasma membrane selenite transport; and polyphosphate metabolic process. Located in plasma membrane. Orthologous to human SVOPL (SVOP like). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See PHO84 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (24037..25800, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The genomic region of the 3' untranslated region (3'UTR) of PHO84, rather than the antisense RNA, promotes gene repression.
Title: The genomic region of the 3' untranslated region (3'UTR) of PHO84, rather than the antisense RNA, promotes gene repression.
Nucleosome Remodeling at the Yeast PHO8 and PHO84 Promoters without the Putatively Essential SWI/SNF Remodeler.
Title: Nucleosome Remodeling at the Yeast PHO8 and PHO84 Promoters without the Putatively Essential SWI/SNF Remodeler.
Leishmania amazonesis orthologue, LamPho84 gene is differentially regulated during parasite life cycle, and plays a role in the success of the parasite throughout its life cycle.
Title: Leishmania amazonensis inorganic phosphate transporter system is increased in the proliferative forms.
Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84
Title: Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84.
The phosphate-binding site residues were shown to be well conserved between all the plant PHT1 proteins and Saccharomyces cerevisiae PHO84.
Title: The conservation of phosphate-binding residues among PHT1 transporters suggests that distinct transport affinities are unlikely to result from differences in the phosphate-binding site.
Molecular dynamics simulations of Pho84 in which a residue adjacent to Tyr(179), Asp(178), is protonated revealed a conformational change in Pho84 from an open, inward-facing state to an occluded state.
Title: Key Residues and Phosphate Release Routes in the Saccharomyces cerevisiae Pho84 Transceptor: THE ROLE OF TYR179 IN FUNCTIONAL REGULATION.
Gcn5 is required for activation of nuclear genes and lifespan extension in the retrograde response.These results, along with the finding that the histone deacetylase Sir2 was required for a robust retrograde response informed a bioinformatics screen that reduced to four the candidate genes causal for longevity .Of the four, only deletion of PHO84 suppressed lifespan extension
Title: Identification of the Target of the Retrograde Response that Mediates Replicative Lifespan Extension in Saccharomyces cerevisiae.
The role of Pho84 in phosphate signalling is discussed. [review]
Title: Conservation of PHO pathway in ascomycetes and the role of Pho84.
PHO84 silencing uses antisense transcription via the promoter rather than the static accumulation of antisense RNAs at the repressed gene. PHO84 antisense RNA acts as a bimodal switch.
Title: Bimodal expression of PHO84 is modulated by early termination of antisense transcription.
trace levels of arsenate (70 ppb), the phosphate reduced the initial rate of uptake, but not the total amount removed. PHO84 mRNA levels were nearly 30 times higher in the engineered strains relative to the control strains
Title: Enhanced arsenate uptake in Saccharomyces cerevisiae overexpressing the Pho84 phosphate transporter.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables manganese ion transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphate transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables phosphate transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables selenite:proton symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables selenite:proton symporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in manganese ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in manganese ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphate ion transport	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphate ion transport	IEA Inferred from Electronic Annotation more info
involved_in phosphate ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in plasma membrane selenite transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in polyphosphate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proton transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in fungal-type vacuole	HDA more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: phosphate transporter PHO84

NP_013583.1

High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

24037..25800 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182486.1 → NP_013583.1 TPA: phosphate transporter PHO84 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013583.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0G2, P25297

UniProtKB/TrEMBL

B3LLD7, B5VP08, C8ZE79, N1P0X4

Conserved Domains (1) summary

TIGR00887
Location:49 → 554

2A0109; phosphate:H+ symporter