RAD10 DNA repair protein RAD10 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD10
Official Full Name: DNA repair protein RAD10
Primary source: SGD:S000004560
Locus tag: YML095C
See related: AllianceGenome:SGD:S000004560; FungiDB:YML095C; VEuPathDB:YML095C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables single-stranded DNA binding activity. Contributes to single-stranded DNA endodeoxyribonuclease activity. Involved in DNA metabolic process. Located in nucleus. Part of nucleotide-excision repair factor 1 complex. Human ortholog(s) of this gene implicated in several diseases, including azoospermia; carcinoma (multiple); cerebrooculofacioskeletal syndrome 4; gastrointestinal system cancer (multiple); and hematologic cancer (multiple). Orthologous to human ERCC1 (ERCC excision repair 1, endonuclease non-catalytic subunit). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See RAD10 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (81481..82113, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RAD10 interactions with RAD1, Msh2-Msh3 and RPA is necessary for 3' non-homologous tail removal.
Title: Coordination of Rad1-Rad10 interactions with Msh2-Msh3, Saw1 and RPA is essential for functional 3' non-homologous tail removal.
Rad1-Rad10 makes distinct contributions to inter-strand crosslink repair (ICLR) depending on cell cycle phase: in G1, Rad1-Rad10 removes ICL via non-nucleotide excision repair (NER), whereas in S/G2, Rad1-Rad10 facilitates NER-independent replication-coupled. Results suggest that NER function in ICLR is conserved from yeast to human and yeast will provide a tractable system to dissect this critical repair pathway.
Title: Distinct roles of XPF-ERCC1 and Rad1-Rad10-Saw1 in replication-coupled and uncoupled inter-strand crosslink repair.
Presented is a model for the role of Rad1-Rad10 cleavage in formation of recombinants between dispersed repeats.
Title: The Rad1-Rad10 nuclease promotes chromosome translocations between dispersed repeats.
yeast Nucleotide Excision Repair protein assembly is step-wise whereas Rad14 required to recruit Rad10 and Rad14 involvement is transient
Title: Rad10-YFP focus induction in response to UV depends on RAD14 in yeast.
observations are consistent with a putative role of Rad10 protein in excising overhanging DNA ends after homology searching and refine the potential role(s) of the Rad1-Rad10 complex in DSB repair in yeast.
Title: Rad10 exhibits lesion-dependent genetic requirements for recruitment to DNA double-strand breaks in Saccharomyces cerevisiae.
Taken together, these results suggest that radiation-induced microhomology-mediated recombination is under the same genetic control as the single-strand annealing apparatus that requires the RAD52, RAD1 and RAD10 genes.
Title: Rad1, rad10 and rad52 mutations reduce the increase of microhomology length during radiation-induced microhomology-mediated illegitimate recombination in saccharomyces cerevisiae.
The Rad10 along with Rad1 and Slx4 promotes efficient repair during gene conversion events involving a single 3' nonhomologous tail
Title: Mutants defective in Rad1-Rad10-Slx4 exhibit a unique pattern of viability during mating-type switching in Saccharomyces cerevisiae.
Data suggest that Saw1 targets Rad1/Rad10 to Rad52-coated recombination intermediates.
Title: Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.
Rad1-Rad10 functions at different stages of GCR formation and there is an alternative pathway for the GCR formation that is independent of Rad1-Rad10
Title: The Rad1-Rad10 complex promotes the production of gross chromosomal rearrangements from spontaneous DNA damage in Saccharomyces cerevisiae.
The ability of Rad1-Rad10 nuclease to associate in a complex with Rad14 is paramount for the targeting of this nuclease to lesion sites in vivo.
Title: Complex formation with damage recognition protein Rad14 is essential for Saccharomyces cerevisiae Rad1-Rad10 nuclease to perform its function in nucleotide excision repair in vivo.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to single-stranded DNA endodeoxyribonuclease activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA amplification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via single-strand annealing, removal of nonhomologous ends	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide-excision repair	IDA Inferred from Direct Assay more info	PubMed
involved_in removal of nonhomologous ends	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of nucleotide-excision repair factor 1 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA repair protein RAD10

NP_013614.1

Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

81481..82113 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182454.1 → NP_013614.1 TPA: DNA repair protein RAD10 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013614.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0J0, P06838

UniProtKB/TrEMBL

A6ZLX2, B3LLG3, B5VP37, C7GV77, C8ZEA9, G2WJW2

Conserved Domains (1) summary

COG5241
Location:1 → 210

RAD10; Nucleotide excision repair endonuclease NEF1, RAD10 subunit [DNA replication, recombination, and repair]