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AI4 intron-encoded DNA endonuclease aI4 [ Saccharomyces cerevisiae S288C ]

Gene ID: 854596, updated on 2-Nov-2024

Summary

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Official Symbol
AI4
Official Full Name
intron-encoded DNA endonuclease aI4
Primary source
SGD:S000007264
Locus tag
Q0065
See related
AllianceGenome:SGD:S000007264; FungiDB:Q0065; VEuPathDB:Q0065
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Saccharomyces cerevisiae S288C (strain: S288C, substrain: FY1679)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables endonuclease activity. Involved in RNA splicing and intron homing. Predicted to be located in mitochondrion. Predicted to be part of respiratory chain complex IV. Orthologous to human MT-CO1 (mitochondrially encoded cytochrome c oxidase I). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See AI4 in Genome Data Viewer
Sequence:
Chromosome: MT; NC_001224.1 (13818..21935)

Chromosome MT - NC_001224.1Genomic Context describing neighboring genes Neighboring gene 15S ribosomal RNA Neighboring gene tRNA-Trp Neighboring gene intron-encoded DNA endonuclease aI5 alpha Neighboring gene cytochrome c oxidase subunit 1 Neighboring gene intron-encoded reverse transcriptase aI1 Neighboring gene intron-encoded reverse transcriptase aI2 Neighboring gene intron-encoded DNA endonuclease aI3 Neighboring gene intron-encoded DNA endonuclease aI5 beta Neighboring gene F1F0 ATP synthase subunit 8

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001224.1

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Maturase and endonuclease functions depend on separate conserved domains of the bifunctional protein encoded by the group I intron aI4 alpha of yeast mitochondrial DNA. Henke RM, et al. EMBO J, 1995 Oct 16. PMID 7588637, Free PMC Article
  2. Single base substitution in an intron of oxidase gene compensates splicing defects of the cytochrome b gene. Dujardin G, et al. Nature, 1982 Aug 12. PMID 6285204
  3. In vivo and in vitro analyses of an intron-encoded DNA endonuclease from yeast mitochondria. Recognition site by site-directed mutagenesis. Sargueil B, et al. Nucleic Acids Res, 1990 Oct 11. PMID 2216759, Free PMC Article
  4. Purification of a site-specific endonuclease, I-Sce II, encoded by intron 4 alpha of the mitochondrial coxI gene of Saccharomyces cerevisiae. Wernette CM, et al. J Biol Chem, 1990 Nov 5. PMID 2172241
  5. Dissecting and analyzing the secondary structure domains of group I introns through the use of chimeric intron constructs. Tanner NK, et al. J Mol Biol, 1995 Oct 6. PMID 7563076

See all (15) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
contributes_to cytochrome-c oxidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cytochrome-c oxidase activity IEA
Inferred from Electronic Annotation
more info
  
enables endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables heme binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA splicing IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in aerobic respiration IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in aerobic respiration IEA
Inferred from Electronic Annotation
more info
  
involved_in intron homing IEA
Inferred from Electronic Annotation
more info
  
involved_in intron homing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in respiratory electron transport chain IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion TAS
Traceable Author Statement
more info
 PubMed 
part_of respiratory chain complex IV IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
intron-encoded DNA endonuclease aI4
NP_009307.2
  • Endonuclease I-SceII; encoded by a mobile group I intron within the mitochondrial COX1 gene; intron is normally spliced by the BI4p maturase but AI4p can mutate to acquire the same maturase activity

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001224.1 Reference assembly

    Range
    13818..21935
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_009307.2 intron-encoded DNA endonuclease aI4 (mitochondrion) [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009307.2

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    A0A0A7P354, P03878, Q02339, Q35798, Q9ZZX2
    Conserved Domains (2) summary
    COG0843
    Location:1 → 555
    CyoB; Heme/copper-type cytochrome/quinol oxidase, subunit 1 [Energy production and conversion]
    pfam00115
    Location:13 → 240
    COX1; Cytochrome C and Quinol oxidase polypeptide I
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P03878.3

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