MYO2 myosin 2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MYO2
Official Full Name: myosin 2
Primary source: SGD:S000005853
Locus tag: YOR326W
See related: AllianceGenome:SGD:S000005853; FungiDB:YOR326W; VEuPathDB:YOR326W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDC66
Summary: Enables actin filament binding activity; calmodulin binding activity; and microfilament motor activity. Involved in several processes, including budding cell apical bud growth; establishment of mitotic spindle orientation; and organelle inheritance. Located in several cellular components, including cellular bud tip; fungal-type vacuole membrane; and mating projection tip. Part of several cellular components, including Myo2p-Vac17p-Vac8p transport complex; filamentous actin; and incipient cellular bud site. Human ortholog(s) of this gene implicated in Griscelli syndrome type 1 and microvillus inclusion disease. Orthologous to several human genes including MYO5A (myosin VA) and MYO5B (myosin VB). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See MYO2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (925721..930445)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cargo Recognition Mechanisms of Yeast Myo2 Revealed by AlphaFold2-Powered Protein Complex Prediction.
Title: Cargo Recognition Mechanisms of Yeast Myo2 Revealed by AlphaFold2-Powered Protein Complex Prediction.
Unlike myo2-E1-Sup1, a previously characterized myo2-E1 suppressor, myo2-E1-Sup2 reverses actomyosin ring contraction defects in vitro and in vivo. Structural analysis of available myosin motor domain conformations suggests that a steric clash in myo2-E1, which is caused by the replacement of a glycine with a bulky arginine, is relieved in myo2-E1-Sup2 by mutation of a tyrosine to a smaller cysteine.
Title: Steric hindrance in the upper 50 kDa domain of the motor Myo2p leads to cytokinesis defects in fission yeast.
Upon arrival at the bud cortex, Vac17 is phosphorylated by Cla4. Cla4-dependent phosphorylation is required for the ubiquitylation and subsequent degradation of Vac17 and the release of the vacuole from Myo2.
Title: Spatial regulation of organelle release from myosin V transport by p21-activated kinases.
spatial and temporal regulation of myosin V in vivo by a head-to-tail interaction is critical for the normal delivery functions of the motor.
Title: Head-to-tail regulation is critical for the in vivo function of myosin V.
These findings reveal a new fast-acting energy conservation strategy halting growth by immobilizing myosin V in a newly described state on selectively stabilized actin cables.
Title: Rapid glucose depletion immobilizes active myosin V on stabilized actin cables.
myosin-V is activated by binding a secretory vesicle and that myosin-V mutations that compromise vesicle binding render the motor constitutively active
Title: Myosin-V is activated by binding secretory cargo and released in coordination with Rab/exocyst function.
Myo2p is not processive on bare actin but highly processive on actin-tropomyosin. Tpm1p supports the most robust processivity.
Title: Tropomyosin is essential for processive movement of a class V myosin from budding yeast.
Myo2p interacts with Pex19p
Title: Pex19p contributes to peroxisome inheritance in the association of peroxisomes to Myo2p.
It was shown that Mmr1 functions as a mitochondria-specific cargo adaptor early in the cell cycle and that Mmr1 binds Myo2 at the site that binds Vac17.
Title: Overlap of cargo binding sites on myosin V coordinates the inheritance of diverse cargoes.
identified the Sec15-binding region on Myo2 and residues on Sec15 required for interaction with Myo2. That Myo2 interacts with Sec15 uncovers additional roles for the exocyst as an adaptor for molecular motors.
Title: Myosin V transports secretory vesicles via a Rab GTPase cascade and interaction with the exocyst complex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables microfilament motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables microfilament motor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within intracellular distribution of mitochondria	IGI Inferred from Genetic Interaction more info	PubMed
involved_in intracellular distribution of mitochondria	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within intracellular distribution of mitochondria	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular distribution of mitochondria	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane addition at site of cytokinesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in membrane addition at site of cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion inheritance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitochondrion inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peroxisome inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peroxisome inheritance	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in vacuole inheritance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vacuole inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuole inheritance	IPI Inferred from Physical Interaction more info	PubMed
involved_in vesicle docking involved in exocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in vesicle transport along actin filament	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle transport along actin filament	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Myo2p-Vac17p-Vac8p transport complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in actin filament bundle	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IEA Inferred from Electronic Annotation more info
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of filamentous actin	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	HDA more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
part_of meiotic nuclear membrane microtubule tethering complex	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
part_of myosin complex	IEA Inferred from Electronic Annotation more info
located_in transport vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in vesicle	IBA Inferred from Biological aspect of Ancestor more info
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: myosin 2

NP_014971.1

Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, lipid droplets, and the mitotic spindle; effector of Ypt11p, a rab-type small GTPase; MYO2 has a paralog, MYO4, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

925721..930445

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183746.1 → NP_014971.1 TPA: myosin 2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014971.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W323, P19524

UniProtKB/TrEMBL

B3LJY7, C7GNT8, N1NXH2

Conserved Domains (1) summary

COG5022
Location:2 → 1567

COG5022; Myosin heavy chain [General function prediction only]