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CLP1 cleavage polyadenylation factor subunit CLP1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 854424, updated on 2-Nov-2024

Summary

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Official Symbol
CLP1
Official Full Name
cleavage polyadenylation factor subunit CLP1
Primary source
SGD:S000005776
Locus tag
YOR250C
See related
AllianceGenome:SGD:S000005776; FungiDB:YOR250C; VEuPathDB:YOR250C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Contributes to RNA binding activity. Involved in mRNA 3'-end processing. Part of mRNA cleavage factor complex. Used to study pontocerebellar hypoplasia. Human ortholog(s) of this gene implicated in pontocerebellar hypoplasia type 10. Orthologous to human CLP1 (cleavage factor polyribonucleotide kinase subunit 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See CLP1 in Genome Data Viewer
Location:
chromosome: XV
Exon count:
1
Sequence:
Chromosome: XV; NC_001147.6 (800970..802307, complement)

Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene Srl1p Neighboring gene anaphase promoting complex subunit 5 Neighboring gene thiosulfate sulfurtransferase Neighboring gene Tma16p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. X-ray crystallographic evidence of an extended binding interface of Pcf11p with Clp1p which is likely to be S. cerevisiae specific.
    Title: Structural basis for ATP loss by Clp1p in a G135R mutant protein.
  2. results support a structural role for the Clp1 P-loop motif. ATP binding by Clp1 likely contributes to CF IA formation and cross-factor interactions during the dynamic process of 3' end formation
    Title: The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3' end formation.
  3. The results define Clp1 as a bridge between CF IA and Cleavage-Polyadenylation Factor and indicate that the Clp1-Pcf11 interaction is modulated by amino acids in the conserved ATP-binding site of Clp1.
    Title: The interaction of Pcf11 and Clp1 is needed for mRNA 3'-end formation and is modulated by amino acids in the ATP-binding site.
  4. CF IA is reconstituted from Rna14, Rna15, Pcf11, and Clp1 proteins in 2:2:1:1 stoichiometry.
    Title: Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology.
  5. Molecular model of a ternary complex of Clp1-ATP and the Clp1-binding region of Pcf11.
    Title: Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
contributes_to RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables polynucleotide 5'-hydroxyl-kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables polynucleotide 5'-hydroxyl-kinase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in mRNA 3'-end processing IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mRNA 3'-end processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA splicing, via endonucleolytic cleavage and ligation IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of mRNA cleavage factor complex IEA
Inferred from Electronic Annotation
more info
  
part_of mRNA cleavage factor complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
cleavage polyadenylation factor subunit CLP1
NP_014893.1
  • Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation and in gene looping which affects reinitiation of transcription; mutations in human ortholog cause cerebellar neurodegeneration

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001147.6 Reference assembly

    Range
    800970..802307 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183669.1 → NP_014893.1  TPA: cleavage polyadenylation factor subunit CLP1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014893.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A6ZP88, D6W2V1, Q08685
    UniProtKB/TrEMBL
    B3LJR8, C7GWD9, N1NWV2
    Conserved Domains (1) summary
    COG5623
    Location:23 → 445
    CLP1; Predicted GTPase subunit of the pre-mRNA cleavage complex [Translation, ribosomal structure and biogenesis]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q08685.1

Gene LinkOut

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