ULS1 translocase ULS1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ULS1
Official Full Name: translocase ULS1
Primary source: SGD:S000005717
Locus tag: YOR191W
See related: AllianceGenome:SGD:S000005717; FungiDB:YOR191W; VEuPathDB:YOR191W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DIS1; RIS1; TID4
Summary: Enables SUMO binding activity. Involved in chromatin assembly or disassembly; mating type switching; and negative regulation of double-strand break repair via nonhomologous end joining. Located in kinetochore and nucleolus. Orthologous to human TTF2 (transcription termination factor 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ULS1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (692475..697334)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data highlights the role of Uls1 in antagonizing Top2 function. Remodelling Top2 binding is thus an important new means by which Snf2 enzymes promote genome stability.
Title: The ATP-dependent chromatin remodelling enzyme Uls1 prevents Topoisomerase II poisoning.
Deletion of ULS1 confers damage tolerance in sgs1 mutants through a Top3-dependent D-loop mediated fork restart pathway
Title: Deletion of ULS1 confers damage tolerance in sgs1 mutants through a Top3-dependent D-loop mediated fork restart pathway.
Yen1 and Uls1 displayed a physical interaction. This interaction was strengthened by SUMO-modification of Yen1.
Title: Helicase/SUMO-targeted ubiquitin ligase Uls1 interacts with the Holliday junction resolvase Yen1.
Uls1 contributes to the pathway where intermediates generated at replication forks are dismantled by Srs2 bound to SUMO-PCNA. Upon ULS1 deletion, accumulating Srs2-SUMO-unable to bind PCNA-takes part in an alternative PCNA-independent recombination repair salvage pathway(s)
Title: DNA Damage Tolerance Pathway Choice Through Uls1 Modulation of Srs2 SUMOylation in Saccharomyces cerevisiae.
deletion of ULS1 restores stability of the rDNA region in sgs1Delta cells.
Title: Swi2/Snf2-like protein Uls1 functions in the Sgs1-dependent pathway of maintenance of rDNA stability and alleviation of replication stress.
End-joining inhibition at telomeres requires the translocase and polySUMO-dependent ubiquitin ligase Uls1.
Title: End-joining inhibition at telomeres requires the translocase and polySUMO-dependent ubiquitin ligase Uls1.
Deletion of ULS1 in cells lacking RAD52 caused a synthetic growth defect accompanied by prolonged S phase and aberrant cell morphology.
Title: The Swi2-Snf2-like protein Uls1 is involved in replication stress response.
In the absence of the Rad54, Rdh54, and Uls1 translocases, Rad51 accumulates spontaneously on chromatin, blocking cell growth and increasing genomic instability. The translocases dissociate Rad51 from sites of nonspecific dsDNA binding during mitosis.
Title: Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth.
A SUMO-binding motif of Ris1p was required for interaction with Ebp2p.
Title: SUMO mediates interaction of Ebp2p, the yeast homolog of Epstein-Barr virus nuclear antigen 1-binding protein 2, with a RING finger protein Ris1p.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent activity, acting on DNA	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables ATP-dependent chromatin remodeler activity	IEA Inferred from Electronic Annotation more info
enables SUMO binding	IPI Inferred from Physical Interaction more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in mating type switching	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: translocase ULS1

NP_014834.1

Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL); required for maintenance of NHEJ inhibition at telomeres; functions at telomeres to translocate and ubiquitinylate poly-sumoylated Rap1p for proteosomal degradation; plays role in antagonizing silencing during mating-type switching; only known STUbL with a translocase activity; contains RING finger domain; relocalizes from nucleus to cytoplasm upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

692475..697334

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183610.1 → NP_014834.1 TPA: translocase ULS1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014834.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2P7, Q08562

UniProtKB/TrEMBL

B3LJM1, C8ZGV1

Conserved Domains (2) summary

COG0553
Location:629 → 1617

HepA; Superfamily II DNA or RNA helicase, SNF2 family [Transcription, Replication, recombination and repair]

cd18008
Location:943 → 1204

DEXDc_SHPRH-like; DEXH-box helicase domain of SHPRH-like proteins