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PSH1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 854102, updated on 11-Sep-2024

Summary

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Official Symbol
PSH1
Primary source
SGD:S000005415
Locus tag
YOL054W
See related
AllianceGenome:SGD:S000005415; FungiDB:YOL054W; VEuPathDB:YOL054W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables ubiquitin-protein transferase activity. Involved in ubiquitin-dependent protein catabolic process. Located in chromosome, centromeric region. Orthologous to human TRIM25 (tripartite motif containing 25). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XV
Exon count:
1
Sequence:
Chromosome: XV; NC_001147.6 (228614..229834)

Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene tRNA-Gly Neighboring gene tRNA-Asn Neighboring gene Aim39p Neighboring gene Ddr2p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Molecular basis for the selective recognition and ubiquitination of centromeric histone H3 by yeast E3 ligase Psh1. Zhou N, et al. J Genet Genomics, 2021 Jun 20. PMID 34217622
  2. Quantitative proteomics and transcriptomics reveal key metabolic processes associated with cotton fiber initiation. Wang XC, et al. J Proteomics, 2015 Jan 30. PMID 25449837
  3. Ubiquitin protease Ubp8 is necessary for S. cerevisiae respiration. Leo M, et al. Biochim Biophys Acta Mol Cell Res, 2018 Aug 2. PMID 30077637
  4. N-terminal Sumoylation of Centromeric Histone H3 Variant Cse4 Regulates Its Proteolysis To Prevent Mislocalization to Non-centromeric Chromatin. Ohkuni K, et al. G3 (Bethesda), 2018 Mar 28. PMID 29432128, Free PMC Article
  5. SAGA DUB-Ubp8 Deubiquitylates Centromeric Histone Variant Cse4. Canzonetta C, et al. G3 (Bethesda), 2015 Nov 27. PMID 26613948, Free PMC Article

See all (76) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Psh1p is required for the faithful inheritance of both centromeric and 2 mum plasmids.
    Title: The Ubiquitin Ligase (E3) Psh1p Is Required for Proper Segregation of both Centromeric and Two-Micron Plasmids in Saccharomyces cerevisiae.
  2. Psh1 and Ubr2 synergistically control the amount of proteins at the kinetochore.
    Title: Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.
  3. Report show that Fpr3 regulates Cse4 proteolysis and the structural changes in CSe4 might be important for its interaction with Psh1.
    Title: Degradation of centromeric histone H3 variant Cse4 requires the Fpr3 peptidyl-prolyl Cis-Trans isomerase.
  4. phosphorylation is crucial in Psh1-assisted control of Cse4 levels and the Psh1-Cse4 association itself functions to prevent Cse4 misincorporation
    Title: Phosphorylation by casein kinase 2 facilitates Psh1 protein-assisted degradation of Cse4 protein.
  5. The interaction between Psh1 and Spt16 is critical for both CENP-A(Cse4) ubiquitylation and its exclusion from euchromatin
    Title: The FACT complex interacts with the E3 ubiquitin ligase Psh1 to prevent ectopic localization of CENP-A.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin-protein transferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ubiquitin-dependent protein catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in chromosome, centromeric region IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
ubiquitin-protein ligase PSH1
NP_014587.1
  • E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates polyubiquitination and degradation of histone H3 variant Cse4p; ubiquitination of Cse4p may be antagonized by Scm3p; interacts with the FACT complex preventing mislocalization of Cse4p to euchromatin independent of Slx5p; formyl-methionine/N-recognin component of N-end rule pathway mediating destruction of formylated cytosolic proteins elevated in response to starvation or stationary phase entry

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001147.6 Reference assembly

    Range
    228614..229834
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183309.1 → NP_014587.1  TPA: ubiquitin-protein ligase PSH1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014587.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W213, Q12161
    UniProtKB/TrEMBL
    A6ZNF6, B3LJ05, C7GK21, C8ZHQ9, N1NVP3
    Conserved Domains (2) summary
    cd16568
    Location:29 → 72
    RING-HC_ScPSH1_like; RING finger, HC subclass, found in Saccharomyces cerevisiae POB3/SPT16 histone-associated protein 1 (ScPSH1), Arabidopsis thaliana Protein KEEP ON GOING (AtKEG) and similar proteins
    cl00874
    Location:149 → 175
    DNA_RNApol_7kD; DNA directed RNA polymerase, 7 kDa subunit
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q12161.1

Gene LinkOut

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