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NAP1 histone chaperone NAP1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 853922, updated on 2-Nov-2024

Summary

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Official Symbol
NAP1
Official Full Name
histone chaperone NAP1
Primary source
SGD:S000001756
Locus tag
YKR048C
See related
AllianceGenome:SGD:S000001756; FungiDB:YKR048C; VEuPathDB:YKR048C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables several functions, including cyclin binding activity; histone binding activity; and unfolded protein binding activity. Involved in several processes, including NLS-bearing protein import into nucleus; nucleosome organization; and positive regulation of transcription elongation by RNA polymerase II. Located in cellular bud neck septin collar. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to several human genes including NAP1L1 (nucleosome assembly protein 1 like 1) and NAP1L2 (nucleosome assembly protein 1 like 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See NAP1 in Genome Data Viewer
Location:
chromosome: XI
Exon count:
1
Sequence:
Chromosome: XI; NC_001143.9 (525387..526640, complement)

Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Pln1p Neighboring gene putative redox protein Neighboring gene Trk2p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Dynamics of nucleosome assembly and effects of DNA methylation. Lee JY, et al. J Biol Chem, 2015 Feb 13. PMID 25550164, Free PMC Article
  2. The synergy between RSC, Nap1 and adjacent nucleosome in nucleosome remodeling. Hsu KW, et al. Biochim Biophys Acta Gene Regul Mech, 2019 Feb. PMID 30593928
  3. Self-association of the yeast nucleosome assembly protein 1. McBryant SJ, et al. Biochemistry, 2004 Aug 17. PMID 15301556
  4. Association states of nucleosome assembly protein 1 and its complexes with histones. Tóth KF, et al. J Biol Chem, 2005 Apr 22. PMID 15687486
  5. Phosphorylation in the accessory domain of yeast histone chaperone protein 1 exposes the nuclear export signal sequence. Ashida S, et al. Proteins, 2022 Feb. PMID 34536244

See all (171) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. an entire reaction scheme for the RSC-mediated nucleosome remodeling process that includes DNA looping, nucleosome ejection, the influence of adjacent nucleosomes, and the coordinated action between Nap1 and RSC.
    Title: The synergy between RSC, Nap1 and adjacent nucleosome in nucleosome remodeling.
  2. A Nap1 dimer provides an acidic binding surface and asymmetrically engages a single H2A-H2B heterodimer.
    Title: Structural evidence for Nap1-dependent H2A-H2B deposition and nucleosome assembly.
  3. Study found that Nap1 efficiently promotes disassembly of adjacent nucleosomes with which RSC collides and not the disassembly of nucleosomes mobilized by RSC. These results suggests a mechanism by which Nap1 is recruited to actively transcribed regions and assists in the passage of the transcription complex through chromatin, and it provides a novel mechanism for the coordinated action of RSC and Nap1.
    Title: Coordinated Action of Nap1 and RSC in Disassembly of Tandem Nucleosomes.
  4. Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo.
    Title: Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus.
  5. these results define the distinct roles for histone chaperones Chz1 and Nap1 to regulate Htz1 incorporation into chromatin.
    Title: Distinct roles for histone chaperones in the deposition of Htz1 in chromatin.
  6. Nap1 and DNA directly compete for H2A-H2B histones. Nap1 stabilizes the fold of H2A-H2B histones
    Title: Chaperone Nap1 shields histone surfaces used in a nucleosome and can put H2A-H2B in an unconventional tetrameric form.
  7. findings show that Nap1 and Vps75 regulate cryptic transcription observed in the absence of Ctk1. Rtt109 acetylation of H3 K9 also plays a role in this pathway, and both Vps75 and Nap1 promote acetylation of H3 in the ORF.
    Title: Histone chaperones Nap1 and Vps75 regulate histone acetylation during transcription elongation.
  8. present evidence for a paradigm in which nucleosome assembly requires the elimination of competing, nonnucleosomal histone-DNA interactions by Nap1.
    Title: The histone chaperone Nap1 promotes nucleosome assembly by eliminating nonnucleosomal histone DNA interactions.
  9. the histone chaperones Nap1 and Chz1 have separate Htz1-dependent and -independent functions
    Title: Nap1 and Chz1 have separate Htz1 nuclear import and assembly functions.
  10. analysis of a thermodynamic model for Nap1-histone interactions
    Title: A thermodynamic model for Nap1-histone interactions.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables chromatin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cyclin binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables enzyme activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables histone binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables histone binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables unfolded protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in NLS-bearing protein import into nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in budding cell bud growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in nucleosome assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nucleosome assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleosome assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleosome disassembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of microtubule polymerization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of transcription elongation by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein localization to cell division site after cytokinesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosomal small subunit biogenesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in cell division site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cellular bud neck IEA
Inferred from Electronic Annotation
more info
  
located_in cellular bud neck septin collar IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chromatin IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
histone chaperone NAP1
NP_012974.1
  • Histone chaperone and assembly chaperone for Rps6a/b; involved in histone exchange, replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; promotes solubility of Rps6a, and 40S ribosomal subunit biogenesis; role in transport of H2A and H2B histones to the nucleus; regulates microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001143.9 Reference assembly

    Range
    525387..526640 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179838.1 → NP_012974.1  TPA: histone chaperone NAP1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012974.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VXA9, P25293, P87196
    UniProtKB/TrEMBL
    A7A008, G2WI76, N1P0X6
    Conserved Domains (1) summary
    pfam00956
    Location:95 → 362
    NAP; Nucleosome assembly protein (NAP)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25293.2

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