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MPE1 cleavage polyadenylation factor subunit MPE1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 853806, updated on 2-Nov-2024

Summary

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Official Symbol
MPE1
Official Full Name
cleavage polyadenylation factor subunit MPE1
Primary source
SGD:S000001542
Locus tag
YKL059C
See related
AllianceGenome:SGD:S000001542; FungiDB:YKL059C; VEuPathDB:YKL059C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables pre-mRNA binding activity and ubiquitin protein ligase activity. Involved in protein polyubiquitination and termination of RNA polymerase II transcription. Located in cytosol. Part of mRNA cleavage and polyadenylation specificity factor complex. Is active in nucleus. Used to study cancer. Orthologous to human RBBP6 (RB binding protein 6, ubiquitin ligase). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See MPE1 in Genome Data Viewer
Location:
chromosome: XI
Exon count:
1
Sequence:
Chromosome: XI; NC_001143.9 (328118..329443, complement)

Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Bli1p Neighboring gene fructose-bisphosphate aldolase FBA1 Neighboring gene transcription initiation factor IIA subunit gamma Neighboring gene Nup120p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF. Rodríguez-Molina JB, et al. Mol Cell, 2022 Jul 7. PMID 35584695, Free PMC Article
  2. Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex. Hill CH, et al. Mol Cell, 2019 Mar 21. PMID 30737185, Free PMC Article
  3. Mpe1, a zinc knuckle protein, is an essential component of yeast cleavage and polyadenylation factor required for the cleavage and polyadenylation of mRNA. Vo LT, et al. Mol Cell Biol, 2001 Dec. PMID 11713271, Free PMC Article
  4. Regulation of the Ysh1 endonuclease of the mRNA cleavage/polyadenylation complex by ubiquitin-mediated degradation. Lee SD, et al. RNA Biol, 2020 May. PMID 32009536, Free PMC Article
  5. Dynamics in Fip1 regulate eukaryotic mRNA 3' end processing. Kumar A, et al. Genes Dev, 2021 Nov 1. PMID 34593603, Free PMC Article

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF.
    Title: Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF.
  2. Determine the structure of Ysh1 bound to Mpe1 and the arrangement of subunits within CPFcore. Data suggest that the active mRNA 3' end processing machinery is a dynamic assembly that is licensed to cleave only when all protein factors come together at the polyadenylation site.
    Title: Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.
  3. Results show that three conserved domains (ubiquitin-like domain, a zinc knuckle, and a RING finger domain) of Mpe1 are important for normal yeast growth and mRNA 3'-end processing.
    Title: Efficient mRNA polyadenylation requires a ubiquitin-like domain, a zinc knuckle, and a RING finger domain, all contained in the Mpe1 protein.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables pre-mRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pre-mRNA binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin protein ligase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in protein polyubiquitination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in protein ubiquitination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in termination of RNA polymerase II transcription IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of mRNA cleavage and polyadenylation specificity factor complex IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
cleavage polyadenylation factor subunit MPE1
NP_012864.1
  • Subunit of CPF cleavage and polyadenylation factor and E3 Ub-ligase; detects polyadenylation signal in nascent pre-mRNA and triggers 3' end formation of mRNA via cleavage and polyadenylation; binds pre-mRNA via zinc knuckle and RING finger; E3 ubiquitin ligase involved in polyubiquitination of endonuclease Ysh1p; contains a ubiquitin-like (UBL) domain, a RNA-binding zinc knuckle motif and a RING finger domain; relocalizes to cytosol in response to hypoxia; homolog of human RBBP6

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001143.9 Reference assembly

    Range
    328118..329443 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179625.1 → NP_012864.1  TPA: cleavage polyadenylation factor subunit MPE1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012864.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VXM8, P35728
    UniProtKB/TrEMBL
    A6ZZQ7, B3LR32, B5VM98, C7GP15, C8ZCB3, G2WHX3, N1P0L5
    Conserved Domains (1) summary
    COG5222
    Location:1 → 441
    COG5222; Uncharacterized conserved protein, contains RING Zn-finger [General function prediction only]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P35728.1

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