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SSH4 Ssh4p [ Saccharomyces cerevisiae S288C ]

Gene ID: 853735, updated on 2-Nov-2024

Summary

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Official Symbol
SSH4
Official Full Name
Ssh4p
Primary source
SGD:S000001607
Locus tag
YKL124W
See related
AllianceGenome:SGD:S000001607; FungiDB:YKL124W; VEuPathDB:YKL124W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
MLF4
Summary
Enables ubiquitin-like ligase-substrate adaptor activity. Involved in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. Located in fungal-type vacuole. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SSH4 in Genome Data Viewer
Location:
chromosome: XI
Exon count:
1
Sequence:
Chromosome: XI; NC_001143.9 (210593..212332)

Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene serine/threonine protein kinase YPK1 Neighboring gene rDNA-binding RNA polymerase I transcriptional factor Neighboring gene signal recognition particle subunit SRP21 Neighboring gene Dgr2p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The immunosuppressive drug leflunomide affects mating-pheromone response and sporulation by different mechanisms in Saccharomyces cerevisiae. Fujimura H. FEMS Microbiol Lett, 2000 Oct 1. PMID 11004400
  2. A selective transmembrane recognition mechanism by a membrane-anchored ubiquitin ligase adaptor. Arines FM, et al. J Cell Biol, 2021 Jan 4. PMID 33351099, Free PMC Article
  3. TORC1 regulates vacuole membrane composition through ubiquitin- and ESCRT-dependent microautophagy. Yang X, et al. J Cell Biol, 2020 Mar 2. PMID 32045480, Free PMC Article
  4. Molecular cloning of Saccharomyces cerevisiae MLF4/SSH4 gene which confers the immunosuppressant leflunomide resistance. Fujimura H. Biochem Biophys Res Commun, 1998 May 19. PMID 9610367
  5. Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies. Léon S, et al. Mol Biol Cell, 2008 Jun. PMID 18367543, Free PMC Article

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Ssh4-Rsp5 quality control system clears membrane proteins mistargeted to the vacuole membrane.
    Title: Rsp5 Ubiquitin ligase-mediated quality control system clears membrane proteins mistargeted to the vacuole membrane.
  2. Ear1p/Ssh4p recruit Rsp5p and assist it in its function at multivesicular bodies by directing the ubiquitylation of specific cargoes.
    Title: Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies.
  3. model in which Ssh4, Rcr2, and presumably Rcr1, function within the endosome-vacuole trafficking pathway, where they affect events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane
    Title: Ssh4, Rcr2 and Rcr1 affect plasma membrane transporter activity in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ubiquitin-like ligase-substrate adaptor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in vesicle-mediated transport IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in endoplasmic reticulum HDA PubMed 
located_in endosome IEA
Inferred from Electronic Annotation
more info
  
located_in endosome membrane IEA
Inferred from Electronic Annotation
more info
  
located_in fungal-type vacuole IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuolar membrane IEA
Inferred from Electronic Annotation
more info
  
located_in vacuole IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Ssh4p
NP_012798.1
  • Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001143.9 Reference assembly

    Range
    210593..212332
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179690.1 → NP_012798.1  TPA: Ssh4p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012798.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A6ZZJ6, D6VX71, P32343
    UniProtKB/TrEMBL
    G2WHR3, N1P0R7
    Conserved Domains (1) summary
    cl02614
    Location:199 → 341
    SPRY; SPRY domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32343.2

Gene LinkOut

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