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ADD66 Add66p [ Saccharomyces cerevisiae S288C ]

Gene ID: 853629, updated on 18-Sep-2024

Summary

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Official Symbol
ADD66
Official Full Name
Add66p
Primary source
SGD:S000001689
Locus tag
YKL206C
See related
AllianceGenome:SGD:S000001689; FungiDB:YKL206C; VEuPathDB:YKL206C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
PBA2; POC2
Summary
Involved in proteasome assembly. Located in cytosol. Orthologous to human PSMG2 (proteasome assembly chaperone 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ADD66 in Genome Data Viewer
Location:
chromosome: XI
Exon count:
1
Sequence:
Chromosome: XI; NC_001143.9 (49006..49809, complement)

Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Cbt1p Neighboring gene Emc3p Neighboring gene Ran GTPase-binding protein LOS1 Neighboring gene Eap1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Mechanism of proteasome gate modulation by assembly chaperones Pba1 and Pba2. Schnell HM, et al. J Biol Chem, 2022 May. PMID 35398095, Free PMC Article
  2. ADD66, a gene involved in the endoplasmic reticulum-associated degradation of alpha-1-antitrypsin-Z in yeast, facilitates proteasome activity and assembly. Scott CM, et al. Mol Biol Cell, 2007 Oct. PMID 17634286, Free PMC Article
  3. Structure of a proteasome Pba1-Pba2 complex: implications for proteasome assembly, activation, and biological function. Stadtmueller BM, et al. J Biol Chem, 2012 Oct 26. PMID 22930756, Free PMC Article
  4. A conserved 20S proteasome assembly factor requires a C-terminal HbYX motif for proteasomal precursor binding. Kusmierczyk AR, et al. Nat Struct Mol Biol, 2011 May. PMID 21499243, Free PMC Article
  5. Structures of chaperone-associated assembly intermediates reveal coordinated mechanisms of proteasome biogenesis. Schnell HM, et al. Nat Struct Mol Biol, 2021 May. PMID 33846632, Free PMC Article

See all (42) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mechanism of proteasome gate modulation by assembly chaperones Pba1 and Pba2.
    Title: Mechanism of proteasome gate modulation by assembly chaperones Pba1 and Pba2.
  2. Ump1 and Pba1-Pba2 promote efficient biogenesis of 20S proteasome core particles from its subunits via 15S intermediates
    Title: Proteasome assembly from 15S precursors involves major conformational changes and recycling of the Pba1-Pba2 chaperone.
  3. ADD66 encodes an approximately 30-kDa soluble, cytosolic protein and that the chymotrypsin-like activity of the proteasome is reduced in add66Delta mutants.
    Title: ADD66, a gene involved in the endoplasmic reticulum-associated degradation of alpha-1-antitrypsin-Z in yeast, facilitates proteasome activity and assembly.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in proteasome assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in proteasome assembly IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in proteasome assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
Add66p
NP_012716.1
  • Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001143.9 Reference assembly

    Range
    49006..49809 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179771.1 → NP_012716.1  TPA: Add66p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012716.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VWZ7, P36040
    UniProtKB/TrEMBL
    A7A083, B3LQQ3, B5VLW0, C7GJ07, C8ZBX4, G2WHI8, N1P7X9
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P36040.1

Gene LinkOut

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