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ENT3 Ent3p [ Saccharomyces cerevisiae S288C ]

Gene ID: 853589, updated on 2-Nov-2024

Summary

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Official Symbol
ENT3
Official Full Name
Ent3p
Primary source
SGD:S000003886
Locus tag
YJR125C
See related
AllianceGenome:SGD:S000003886; FungiDB:YJR125C; VEuPathDB:YJR125C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables clathrin binding activity; phosphatidylinositol-3,5-bisphosphate binding activity; and phosphatidylinositol-3-phosphate binding activity. Involved in actin cytoskeleton organization; cytosolic transport; and late endosome to vacuole transport via multivesicular body sorting pathway. Located in endosome. Part of clathrin vesicle coat. Orthologous to several human genes including CLINT1 (clathrin interactor 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See ENT3 in Genome Data Viewer
Location:
chromosome: X
Exon count:
1
Sequence:
Chromosome: X; NC_001142.9 (654737..655963, complement)

Chromosome X - NC_001142.9Genomic Context describing neighboring genes Neighboring gene ribosomal 40S subunit protein S5 Neighboring gene uncharacterized protein Neighboring gene putative zinc metalloprotease Neighboring gene Rsf2p Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body. Friant S, et al. Dev Cell, 2003 Sep. PMID 12967568
  2. Epsin N-terminal homology domains bind on opposite sides of two SNAREs. Wang J, et al. Proc Natl Acad Sci U S A, 2011 Jul 26. PMID 21746902, Free PMC Article
  3. Distinct functional domains of the epsin-related Ent5p, a cargo adaptor for the SNARE Tlg2p in transport between endosomes and Golgi. Petersen L, et al. Traffic, 2023 Oct. PMID 37434343
  4. Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body. Eugster A, et al. Mol Biol Cell, 2004 Jul. PMID 15107463, Free PMC Article
  5. Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 . Fang P, et al. Biochemistry, 2010 Sep 14. PMID 20704189

See all (57) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Here we show not all acidic-phenylalanine motifs in Ent3 are active for yGGA2_GAE domain binding
    Title: Structural basis for the specificity of the GAE domain of yGGA2 for its accessory proteins Ent3 and Ent5 .
  2. Ent3p is a connector between PtdIns(3,5)P(2)- and the Vps27p-ubiquitin-driven sorting machinery at the multivesicular body.
    Title: Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body.
  3. the clathrin adaptors AP-1, Ent3p, Ent5p, and the Ggas cooperate in different ways to sort proteins between the TGN and the endosomes
    Title: Ent3p and Ent5p exhibit cargo-specific functions in trafficking proteins between the trans-Golgi network and the endosomes in yeast.
  4. These results differentiate Ent3p and Ent5p function in vivo, suggesting that Ent3p acts primarily with Gga proteins, whereas Ent5p acts with both AP-1 and Gga proteins but is more critical for AP-1-mediated transport.
    Title: Distinct roles for TGN/endosome epsin-like adaptors Ent3p and Ent5p.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables clathrin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables clathrin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipid binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylinositol-3,5-bisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphatidylinositol-3-phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phospholipid binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Golgi to endosome transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in Golgi to endosome transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin cytoskeleton organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in early endosome to Golgi transport IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in endocytosis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in late endosome to vacuole transport via multivesicular body sorting pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in multivesicular body sorting pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
part_of clathrin vesicle coat IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of clathrin vesicle coat IDA
Inferred from Direct Assay
more info
 PubMed 
located_in clathrin-coated vesicle membrane IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasmic vesicle IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
is_active_in endosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
Ent3p
NP_012659.1
  • Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001142.9 Reference assembly

    Range
    654737..655963 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181783.1 → NP_012659.1  TPA: Ent3p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012659.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VWU4, P47160
    UniProtKB/TrEMBL
    A6ZQ81, B3LQK9, B5VLR7, C7GM87, C8ZBS3, G2WHC8, N1P1S3
    Conserved Domains (1) summary
    cd16992
    Location:32 → 152
    ENTH_Ent3; Epsin N-Terminal Homology (ENTH) domain of Yeast Ent3 and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P47160.1

Gene LinkOut

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