SRS2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SRS2
Primary source: SGD:S000003628
Locus tag: YJL092W
See related: AllianceGenome:SGD:S000003628; FungiDB:YJL092W; VEuPathDB:YJL092W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HPR5
Summary: Enables 3'-5' DNA helicase activity and enzyme activator activity. Involved in several processes, including DNA metabolic process; DNA recombinase disassembly; and regulation of DNA metabolic process. Located in nucleus. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (257423..260947)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases.
Title: Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases.
Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.
Title: Genetic Dissection of Budding Yeast PCNA Mutations Responsible for the Regulated Recruitment of Srs2 Helicase.
Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.
Title: Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments.
Prevention of unwanted recombination at damaged replication forks.
Title: Prevention of unwanted recombination at damaged replication forks.
Srs2 C-terminal Rad51 interaction domain is essential for crossover control in S. cerevisiae.
Title: Role of the Srs2-Rad51 Interaction Domain in Crossover Control in Saccharomyces cerevisiae.
DNA helicase domain of Saccharomyces cerevisiae yeast Srs2 protein is needed to deal with DNA damage and assist in DNA replication during vegetative growth and in meiosis. The interactions of Srs2 with Rad51 Recombinase and proliferating cell nuclear Antigen PCNA are dispensable for the main role of Srs2.
Title: The Main Role of Srs2 in DNA Repair Depends on Its Helicase Activity, Rather than on Its Interactions with PCNA or Rad51.
Two main activities of Srs2, strippase and helicase, counteract toxic joint intermediates formed during break-induced replication by preventing their formation and promoting their disruption, respectively.
Title: Break-induced replication promotes formation of lethal joint molecules dissolved by Srs2.
Srs2 rapidly disrupts small heteroduplex DNA joints bound to Rad51 filaments.
Title: Dissociation of Rad51 Presynaptic Complexes and Heteroduplex DNA Joints by Tandem Assemblies of Srs2.
These data establish a biochemical mechanism for the role of Srs2 in crossover suppression by promoting synthesis-dependent strand annealing through disruption of DNA polymerase delta-extended D-loops.
Title: Srs2 promotes synthesis-dependent strand annealing by disrupting DNA polymerase δ-extending D-loops.
Srs2 interaction with PCNA allows the helicase activity to unwind fork-blocking CAG/CTG hairpin structures to prevent DNA breaks during DNA replication. Independently of PCNA binding, Srs2 also displaces Rad51 from nascent strands to prevent recombination-dependent repeat expansions and contractions.
Title: Differential requirement of Srs2 helicase and Rad51 displacement activities in replication of hairpin-forming CAG/CTG repeats.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA protection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombinase disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA recombinase disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IDA Inferred from Direct Assay more info	PubMed
involved_in meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of double-strand break repair via homologous recombination	IDA Inferred from Direct Assay more info	PubMed
involved_in recombinational repair	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA helicase SRS2

NP_012443.1

EC 5.6.2.4

DNA helicase and DNA-dependent ATPase; role in DNA repair and checkpoint recovery, in the proper timing of commitment to meiotic recombination and the Meiosis I to II transition; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; stimulates Mus81p-Mms4p endonuclease activity independent of catalytic activity; ATPase and ssDNA translocating motor activities inhibited by Dmc1p; functional homolog of human RTEL1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

257423..260947

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181525.1 → NP_012443.1 TPA: DNA helicase SRS2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012443.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VW92, P12954

UniProtKB/TrEMBL

B3LQ21, C7GR22, C8ZBC1, N1P8F3

Conserved Domains (1) summary

COG0210
Location:13 → 750

UvrD; Superfamily I DNA or RNA helicase [Replication, recombination and repair]