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CBF2 Cbf2p [ Saccharomyces cerevisiae S288C ]

Gene ID: 853041, updated on 18-Sep-2024

Summary

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Official Symbol
CBF2
Official Full Name
Cbf2p
Primary source
SGD:S000003372
Locus tag
YGR140W
See related
AllianceGenome:SGD:S000003372; FungiDB:YGR140W; VEuPathDB:YGR140W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
CEP2; CSL5; CTF14; NDC10
Summary
Enables DNA binding activity, bending and centromeric DNA binding activity. Involved in mitotic spindle elongation and septin ring assembly. Located in condensed chromosome, centromeric region and spindle midzone. Part of CBF3 complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See CBF2 in Genome Data Viewer
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (767429..770299)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene Lsb1p Neighboring gene spermine transporter Neighboring gene Vps62p Neighboring gene Btn2p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases. Perriches T, et al. J Biol Chem, 2012 Feb 10. PMID 22215672, Free PMC Article
  2. Modeling stem cell asymmetry in yeast. Thorpe PH, et al. Cold Spring Harb Symp Quant Biol, 2008. PMID 19022748
  3. Three-dimensional electron microscopy analysis of ndc10-1 mutant reveals an aberrant organization of the mitotic spindle and spindle pole body defects in Saccharomyces cerevisiae. Romao M, et al. J Struct Biol, 2008 Jul. PMID 18515145
  4. Analysis of the distribution of the kinetochore protein Ndc10p in Saccharomyces cerevisiae using 3-D modeling of mitotic spindles. Müller-Reichert T, et al. Chromosoma, 2003 Apr. PMID 12707779
  5. Is the yeast anaphase promoting complex needed to prevent re-replication during G2 and M phases? Pichler S, et al. EMBO J, 1997 Oct 1. PMID 9312056, Free PMC Article

See all (154) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation.
    Title: Structural and dynamic mechanisms of CBF3-guided centromeric nucleosome formation.
  2. Exposure of bipartite hydrophobic signal triggers nuclear quality control of Ndc10 at the endoplasmic reticulum/nuclear envelope.
    Title: Exposure of bipartite hydrophobic signal triggers nuclear quality control of Ndc10 at the endoplasmic reticulum/nuclear envelope.
  3. Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases.
    Title: Structure of yeast kinetochore Ndc10 DNA-binding domain reveals unexpected evolutionary relationship to tyrosine recombinases.
  4. Localization of Cse4 to centromeres and the assembly activity depend on an evolutionarily conserved core motif in Scm3, but localization of the CBF3 subunit Ndc10 to centromeres does not depend on this motif.
    Title: Scm3 is a centromeric nucleosome assembly factor.
  5. Ndc10p is also essential for spindle pole body maturation and mitotic spindle integrity.
    Title: Three-dimensional electron microscopy analysis of ndc10-1 mutant reveals an aberrant organization of the mitotic spindle and spindle pole body defects in Saccharomyces cerevisiae.
  6. Ndc10p may play a direct role in maintaining spindle stability during anaphase and coordinates the completion of cell division after chromosome segregation
    Title: The kinetochore protein Ndc10p is required for spindle stability and cytokinesis in yeast.
  7. Phosphorylation of the middle region of Bir1 is required to bring Ndc10 to the spindle at anaphase, which is required for full spindle elongation.
    Title: Phosphorylation of the chromosomal passenger protein Bir1 is required for localization of Ndc10 to the spindle during anaphase and full spindle elongation.
  8. Ndc10/Scm3 binds to centromeric DNA, which is in turn essential for targeting Cse4 to centromeres.
    Title: Scm3 is essential to recruit the histone h3 variant cse4 to centromeres and to maintain a functional kinetochore.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding, bending IDA
Inferred from Direct Assay
more info
 PubMed 
enables centromeric DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in chromosome segregation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic spindle elongation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in septin ring assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of CBF3 complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in chromosome, centromeric region IEA
Inferred from Electronic Annotation
more info
  
located_in condensed chromosome, centromeric region IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in spindle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle midzone IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle pole body IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Cbf2p
NP_011656.3
  • Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    767429..770299
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181269.3 → NP_011656.3  TPA: Cbf2p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011656.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VUS0, E9P954, P32504
    UniProtKB/TrEMBL
    B3LI90, C8Z933, N1P5X8
    Conserved Domains (2) summary
    cd11602
    Location:29 → 506
    Ndc10; Ndc10 component of the yeast centromere-binding factor 3
    pfam12550
    Location:843 → 922
    GCR1_C; Transcriptional activator of glycolytic enzymes
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32504.1

Gene LinkOut

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