DAM1 Dam1p [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: DAM1
Official Full Name: Dam1p
Primary source: SGD:S000003345
Locus tag: YGR113W
See related: AllianceGenome:SGD:S000003345; FungiDB:YGR113W; VEuPathDB:YGR113W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to microtubule plus-end binding activity. Involved in attachment of spindle microtubules to kinetochore; positive regulation of attachment of spindle microtubules to kinetochore; and positive regulation of microtubule polymerization. Part of DASH complex. [provided by Alliance of Genome Resources, Apr 2022]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See DAM1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (718893..719924)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.
Title: Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.
Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.
Title: Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.
Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
Title: Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
Our results suggest the stoichiometry of outer kinetochore submodules is strongly influenced by factors at the kinetochore-microtubule interface such as Fin1 and Dam1, and phosphorylation by cyclin-dependent kinase.
Title: The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors.
Dam1 promotes centromere clustering through stabilization of the kinetochore integrity.
Title: Outer kinetochore protein Dam1 promotes centromere clustering in parallel with Slk19 in budding yeast.
In metaphase, approximately 40% of the Dam1C/DASH assemblies are complete rings; the rest are partial rings. Ring completeness and binding position along the microtubule are sensitive to kinetochore attachment and tension, respectively.
Title: Electron cryotomography analysis of Dam1C/DASH at the kinetochore-spindle interface in situ.
regulation of Dam1 phosphorylation imposed by Ipl1 kinase is critical for faithful chromosome segregation.
Title: The phosphorylation of a kinetochore protein Dam1 by Aurora B/Ipl1 kinase promotes chromosome bipolar attachment in yeast.
PKA site of Dam1p participates in regulating kinetochore activity.
Title: Glucose Signaling Is Connected to Chromosome Segregation Through Protein Kinase A Phosphorylation of the Dam1 Kinetochore Subunit in Saccharomycescerevisiae.
One meiotic role of Mps1 is to stabilize connections that have been established between kinetochores and microtubles by phosphorylating Dam1.
Title: Mps1 promotes chromosome meiotic chromosome biorientation through Dam1.
this study reports the cryo-electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis.
Title: Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.

Submit: New GeneRIF Correction See all GeneRIFs (31)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to microtubule binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to microtubule plus-end binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in attachment of spindle microtubules to kinetochore	IDA Inferred from Direct Assay more info	PubMed
involved_in attachment of spindle microtubules to kinetochore	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in centromere clustering	IDA Inferred from Direct Assay more info	PubMed
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid biorientation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of attachment of spindle microtubules to kinetochore	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of microtubule polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of DASH complex	IBA Inferred from Biological aspect of Ancestor more info
part_of DASH complex	IDA Inferred from Direct Assay more info	PubMed
part_of DASH complex	IEA Inferred from Electronic Annotation more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in kinetochore	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle	IEA Inferred from Electronic Annotation more info
is_active_in mitotic spindle polar microtubule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mitotic spindle pole body	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: Dam1p

NP_011628.4

Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p and Pse1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization, thereby aiding in chromosome segregation; N-terminal staple region is important for self-assembly of the Dam1 complex higher order ring structure that forms around microtubules; Ipl1p target for regulating kinetochore-MT attachments