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RNA15 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852838, updated on 11-Sep-2024

Summary

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Official Symbol
RNA15
Primary source
SGD:S000003012
Locus tag
YGL044C
See related
AllianceGenome:SGD:S000003012; FungiDB:YGL044C; VEuPathDB:YGL044C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables mRNA binding activity. Involved in mRNA polyadenylation; pre-mRNA cleavage required for polyadenylation; and response to DNA damage checkpoint signaling. Part of mRNA cleavage factor complex. Orthologous to human CSTF2 (cleavage stimulation factor subunit 2) and CSTF2T (cleavage stimulation factor subunit 2 tau variant). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (416146..417036, complement)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene tRNA-Val Neighboring gene Rim8p Neighboring gene transcription elongation factor DST1 Neighboring gene uncharacterized protein Neighboring gene Dpc13p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structure of the Rna15 RRM-RNA complex reveals the molecular basis of GU specificity in transcriptional 3'-end processing factors. Pancevac C, et al. Nucleic Acids Res, 2010 May. PMID 20097654, Free PMC Article
  2. Multipurpose vectors designed for the fast generation of N- or C-terminal epitope-tagged proteins. Cullin C, et al. Yeast, 1994 Jan. PMID 7515538
  3. Crystal structure of the Rna14-Rna15 complex. Paulson AR, et al. RNA, 2012 Jun. PMID 22513198, Free PMC Article
  4. Chemical shift assignments of a minimal Rna14p/Rna15p heterodimer from the yeast cleavage factor IA complex. Mackereth CD. Biomol NMR Assign, 2011 Apr. PMID 20967574
  5. Novel protein-protein contacts facilitate mRNA 3'-processing signal recognition by Rna15 and Hrp1. Leeper TC, et al. J Mol Biol, 2010 Aug 20. PMID 20600122, Free PMC Article

See all (99) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. CF IA is reconstituted from Rna14, Rna15, Pcf11, and Clp1 proteins in 2:2:1:1 stoichiometry.
    Title: Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology.
  2. Data show that mutation of residues within the minimal heterodimer region inhibit association of full-length Rna14p and Rna15p.
    Title: Locked tether formation by cooperative folding of Rna14p monkeytail and Rna15p hinge domains in the yeast CF IA complex.
  3. The NMR structure of the 34 -kDa ternary complex of the RNA recognition motif (RRM) domains of Hrp1 and Rna15 bound to two neighboring elements upstream of the cleavage site
    Title: Novel protein-protein contacts facilitate mRNA 3'-processing signal recognition by Rna15 and Hrp1.
  4. after inhibition of CFIA components Rna14p and Rna15p, the snoRNP proteins Nop1p, Nop58p, and Gar1p delocalize from the nucleolus and accumulate in discrete nucleoplasmic foci
    Title: Inactivation of cleavage factor I components Rna14p and Rna15p induces sequestration of small nucleolar ribonucleoproteins at discrete sites in the nucleus.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables mRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables mRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in mRNA 3'-end processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA processing IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of mRNA cleavage and polyadenylation specificity factor complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mRNA cleavage stimulating factor complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Rna15p
NP_011471.1
  • Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    416146..417036 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180909.1 → NP_011471.1  TPA: Rna15p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011471.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VU95, P25299
    UniProtKB/TrEMBL
    A6ZUV2, B3LIQ4, C7GS23, C8Z8J9, G2WE51, N1P1Y1
    Conserved Domains (2) summary
    pfam14304
    Location:255 → 291
    CSTF_C; Transcription termination and cleavage factor C-terminal
    cd12398
    Location:18 → 94
    RRM_CSTF2_RNA15_like; RNA recognition motif (RRM) found in cleavage stimulation factor subunit 2 (CSTF2), yeast ortholog mRNA 3'-end-processing protein RNA15 and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25299.1

Gene LinkOut

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