MMS2 E2 ubiquitin-conjugating protein MMS2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MMS2
Official Full Name: E2 ubiquitin-conjugating protein MMS2
Primary source: SGD:S000003055
Locus tag: YGL087C
See related: AllianceGenome:SGD:S000003055; FungiDB:YGL087C; VEuPathDB:YGL087C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to ubiquitin-protein transferase activity. Involved in several processes, including free ubiquitin chain polymerization; postreplication repair; and protein targeting to vacuolar membrane. Located in fungal-type vacuole membrane and nucleus. Part of ubiquitin conjugating enzyme complex. Orthologous to human PEDS1-UBE2V1 (PEDS1-UBE2V1 readthrough); UBE2V1 (ubiquitin conjugating enzyme E2 V1); and UBE2V2 (ubiquitin conjugating enzyme E2 V2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See MMS2 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 2

Sequence:: Chromosome: VII; NC_001139.9 (346406..346904, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ubc13-Mms2 cooperates with a family of RING E3 proteins in budding yeast membrane protein sorting.
Title: Ubc13-Mms2 cooperates with a family of RING E3 proteins in budding yeast membrane protein sorting.
The error-free post-replication repair pathway mediated by Mms2p suppresses dimer-induced recombination between homologs, possibly by channeling replication-blocking lesions into recombination between sister chromatids.
Title: Recombination between homologous chromosomes induced by unrepaired UV-generated DNA damage requires Mus81p and is suppressed by Mms2p.
The authors demonstrate that in the absence of PCNA SUMOylation, Mms2-Ubc13 and Rad5 may, independently of each other, function in the stimulation of translesion DNA synthesis.
Title: The roles of PCNA SUMOylation, Mms2-Ubc13 and Rad5 in translesion DNA synthesis in Saccharomyces cerevisiae.
Rad6-Rad18 and Ubc13-Mms2-Rad5 act sequentially and mediate proliferating cell nuclear antigen poly-ubiquitylation by stepwise addition of ubiquitin monomers.
Title: Mechanistic analysis of PCNA poly-ubiquitylation by the ubiquitin protein ligases Rad18 and Rad5.
Mms2/Ubc13-dependent modifications are a distinct subset of those catalyzed by Ubc4.
Title: Yeast Chfr homologs retard cell cycle at G1 and G2/M via Ubc4 and Ubc13/Mms2-dependent ubiquitination.
role in polyubiquitination of PCNA
Title: Rad18/Rad5/Mms2-mediated polyubiquitination of PCNA is implicated in replication completion during replication stress.
Study suggests that Rad18/Rad5/Mms2 and Mgs1 are required to promote replication when forks are destabilized or stalled due to defects in Pol delta.
Title: Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31.
The structure reveals the key role of Mms2 in allowing selective insertion of Lys63 into the Ubc13 active site and suggests a molecular model for polyubiquitin chain elongation.
Title: Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation.
the ubiquitin binding site of Mms2 is necessary for error-free lesion bypass in the RAD6 pathway
Title: Ubiquitin binding site of the ubiquitin E2 variant (UEV) protein Mms2 is required for DNA damage tolerance in the yeast RAD6 pathway.
In contrast to the role of Rad5 in postreplicational repair, however, where its function is coupled with that of Mms2-Ubc13, Rad5 function in translesion synthesis would be largely independent of this ubiquitin-conjugating enzyme complex
Title: Mms2-Ubc13-dependent and -independent roles of Rad5 ubiquitin ligase in postreplication repair and translesion DNA synthesis in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ligase activity	IEA Inferred from Electronic Annotation more info
contributes_to ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in free ubiquitin chain polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in postreplication repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in postreplication repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in postreplication repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein K63-linked ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IPI Inferred from Physical Interaction more info	PubMed
involved_in protein targeting to vacuolar membrane	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of ubiquitin conjugating enzyme complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ubiquitin conjugating enzyme complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: E2 ubiquitin-conjugating protein MMS2

NP_011428.1

Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

346406..346904 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180952.1 → NP_011428.1 TPA: E2 ubiquitin-conjugating protein MMS2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011428.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU57, P53152

UniProtKB/TrEMBL

A6ZU92, B3LHF3, C8Z8G1, G2WE12, N1P1V0

Conserved Domains (1) summary

COG5078
Location:1 → 137

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]