CDC20 ubiquitin-protein transferase activating protein CDC20 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CDC20
Official Full Name: ubiquitin-protein transferase activating protein CDC20
Primary source: SGD:S000003084
Locus tag: YGL116W
See related: AllianceGenome:SGD:S000003084; FungiDB:YGL116W; VEuPathDB:YGL116W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PAC5
Summary: Enables anaphase-promoting complex binding activity and ubiquitin ligase activator activity. Involved in anaphase-promoting complex-dependent catabolic process; positive regulation of cellular protein metabolic process; and regulation of nuclear division. Located in nuclear periphery. Part of anaphase-promoting complex and mitotic checkpoint complex. Used to study melanoma. Orthologous to several human genes including CDC20 (cell division cycle 20). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See CDC20 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (289809..291641)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Small N-terminal regions of both Cdc20 (RxxL-type D box) and Cdc5 (KEN motif) were identified as transferable degradation domains (degrons) that mediated efficient targeting by APC/C-Cdh1 (anaphase-promoting complex/cyclosome-Cdh1).
Title: Insights into the cellular mechanism of the yeast ubiquitin ligase APC/C-Cdh1 from the analysis of in vivo degrons.
Bub3 has a previously unknown function at the kinetochore in activating APC/C-Cdc20 for normal mitotic progression.
Title: Bub3 promotes Cdc20-dependent activation of the APC/C in S. cerevisiae.
Both catalysis of Mad1:C-Mad2 at the kinetochores and Mad2-Cdc20 binding away from kinetochores are required for a proficient checkpoint.
Title: Role of the Mad2 dimerization interface in the spindle assembly checkpoint independent of kinetochores.
study analyzed the role of Cdc20 autoubiquitination in the spindle assembly checkpoint
Title: The APC/C subunit Mnd2/Apc15 promotes Cdc20 autoubiquitination and spindle assembly checkpoint inactivation.
This study finds that the majority of Cdc20 turnover does not involve a second activator molecule but instead depends on in cis Cdc20 autoubiquitination while it is bound to its activator-binding site on the anaphase-promoting complex (APC) core.
Title: Ubiquitination of Cdc20 by the APC occurs through an intramolecular mechanism.
Mad3 is required for the stable binding of Mad2 to Cdc20 in vivo, which is sufficient to inhibit APC activity and is the most downstream event in spindle checkpoint activation.
Title: Mad2 and Mad3 cooperate to arrest budding yeast in mitosis.
Cdk1 promotes kinetochore bi-orientation during recovery from spindle checkpoint arrest by restraining premature spindle elongation & extinguishing SAC signalling. Cdk1 is essential for sustaining the expression of Cdc20.
Title: Cdk1 promotes kinetochore bi-orientation and regulates Cdc20 expression during recovery from spindle checkpoint arrest.
data suggest important roles of ubiquitination in mediating IGF-1R signaling and degradation
Title: Mad3 KEN boxes mediate both Cdc20 and Mad3 turnover, and are critical for the spindle checkpoint.
These results indicate that Ume6p degradation is required for normal meiotic gene induction and meiotic progression.
Title: Meiosis-specific destruction of the Ume6p repressor by the Cdc20-directed APC/C.
model aimed at unifying previous analyses of checkpoint function by focusing on the Mad3-Cdc20 interaction
Title: Mad3p, a pseudosubstrate inhibitor of APCCdc20 in the spindle assembly checkpoint.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables anaphase-promoting complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables anaphase-promoting complex binding	IDA Inferred from Direct Assay more info	PubMed
enables anaphase-promoting complex binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin ligase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin ligase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activator activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in anaphase-promoting complex-dependent catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in anaphase-promoting complex-dependent catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IPI Inferred from Physical Interaction more info	PubMed
involved_in positive regulation of anaphase-promoting complex-dependent catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of anaphase-promoting complex-dependent catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of anaphase-promoting complex-dependent catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic metaphase/anaphase transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
involved_in regulation of meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of anaphase-promoting complex	IBA Inferred from Biological aspect of Ancestor more info
part_of anaphase-promoting complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	HDA more info	PubMed
part_of mitotic checkpoint complex	IDA Inferred from Direct Assay more info	PubMed
part_of mitotic checkpoint complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nuclear periphery	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ubiquitin-protein transferase activating protein CDC20

NP_011399.1

Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

289809..291641

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180981.1 → NP_011399.1 TPA: ubiquitin-protein transferase activating protein CDC20 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011399.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU31, P26309

UniProtKB/TrEMBL

G2WDY5, N1P3H1

Conserved Domains (2) summary

COG2319
Location:240 → 560

WD40; WD40 repeat [General function prediction only]

sd00039
Location:265 → 301

7WD40; WD40 repeat [structural motif]