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PRP5 DEAD-box RNA helicase PRP5 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852539, updated on 2-Nov-2024

Summary

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Official Symbol
PRP5
Official Full Name
DEAD-box RNA helicase PRP5
Primary source
SGD:S000000441
Locus tag
YBR237W
See related
AllianceGenome:SGD:S000000441; FungiDB:YBR237W; VEuPathDB:YBR237W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
RNA5
Summary
Enables ATP-dependent activity, acting on RNA. Involved in mRNA branch site recognition. Located in nucleus. Orthologous to human DDX46 (DEAD-box helicase 46). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See PRP5 in Genome Data Viewer
Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (691969..694518)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Vhc1p Neighboring gene mRNA (guanine-N7)-methyltransferase Neighboring gene uncharacterized protein Neighboring gene Ert1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. PRP5: a helicase-like protein required for mRNA splicing in yeast. Dalbadie-McFarland G, et al. Proc Natl Acad Sci U S A, 1990 Jun. PMID 2349233, Free PMC Article
  2. The Saccharomyces cerevisiae Prp5 protein has RNA-dependent ATPase activity with specificity for U2 small nuclear RNA. O'Day CL, et al. J Biol Chem, 1996 Dec 27. PMID 8969184
  3. Crystal structure of Prp5p reveals interdomain interactions that impact spliceosome assembly. Zhang ZM, et al. Cell Rep, 2013 Dec 12. PMID 24290758
  4. Probing interactions between the U2 small nuclear ribonucleoprotein and the DEAD-box protein, Prp5. Abu Dayyeh BK, et al. J Biol Chem, 2002 Jun 7. PMID 11927574
  5. Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly. Beier DH, et al. Nucleic Acids Res, 2019 Nov 18. PMID 31712821, Free PMC Article

See all (63) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural insights into how Prp5 proofreads the pre-mRNA branch site.
    Title: Structural insights into how Prp5 proofreads the pre-mRNA branch site.
  2. Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription.
    Title: Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription.
  3. different HSH155 alleles elicit disparate effects on splicing: some increase the fidelity of BS selection while others decrease fidelity. Our data support a model wherein conformational changes in SF3b1 promote U2 association with the BS independently of the action of the DEAD-box ATPase Prp5. We propose that SF3b1 functions to stabilize weak U2/BS duplexes to drive spliceosome assembly and splicing
    Title: SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast.
  4. Taken together, our results indicate that the Psis in U2 snRNA contribute to pre-mRNA splicing by directly altering the binding/ATPase activity of Prp5.
    Title: Pseudouridines in U2 snRNA stimulate the ATPase activity of Prp5 during spliceosome assembly.
  5. Prp5 binds to the spliceosome in association with U2 by interacting with the BSL and is released upon the base-pairing of U2 with the branch site to allow the recruitment of the tri-snRNP.
    Title: A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent activity, acting on RNA IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA branch site recognition IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA splicing, via spliceosome IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ribonucleoprotein complex biogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in nucleus HDA PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
DEAD-box RNA helicase PRP5
NP_009796.1
  • RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    691969..694518
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178585.1 → NP_009796.1  TPA: DEAD-box RNA helicase PRP5 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009796.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQN3, P21372
    UniProtKB/TrEMBL
    G2W9P0
    Conserved Domains (3) summary
    COG0513
    Location:227 → 731
    SrmB; Superfamily II DNA and RNA helicase [Replication, recombination and repair]
    cd17953
    Location:244 → 468
    DEADc_DDX46; DEAD-box helicase domain of DEAD box protein 46
    cd22474
    Location:749 → 837
    KH-I_PRP5_like; type I K homology (KH) RNA-binding domain found in fungal pre-mRNA-processing ATP-dependent RNA helicase PRP5 and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P21372.1

Gene LinkOut

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