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AME1 Ame1p [ Saccharomyces cerevisiae S288C ]

Gene ID: 852512, updated on 18-Sep-2024

Summary

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Official Symbol
AME1
Official Full Name
Ame1p
Primary source
SGD:S000000415
Locus tag
YBR211C
See related
AllianceGenome:SGD:S000000415; FungiDB:YBR211C; VEuPathDB:YBR211C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
ARP100
Summary
Involved in attachment of spindle microtubules to kinetochore and protein localization to kinetochore. Located in kinetochore. Part of COMA complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See AME1 in Genome Data Viewer
Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (646158..647132, complement)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene tRNA-Glu Neighboring gene Erv15p Neighboring gene Ngr1p Neighboring gene bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase MET8

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae. Ross-Macdonald P, et al. Proc Natl Acad Sci U S A, 1997 Jan 7. PMID 8990184, Free PMC Article
  2. Spindle checkpoint maintenance requires Ame1 and Okp1. Pot I, et al. Cell Cycle, 2005 Oct. PMID 16177574
  3. Cdc4 phospho-degrons allow differential regulation of Ame1(CENP-U) protein stability across the cell cycle. Böhm M, et al. Elife, 2021 Jul 26. PMID 34308839, Free PMC Article
  4. Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae. Boeckmann L, et al. Mol Biol Cell, 2013 Jun. PMID 23637466, Free PMC Article
  5. The COMA complex is required for Sli15/INCENP-mediated correction of defective kinetochore attachments. Knockleby J, et al. Cell Cycle, 2009 Aug 15. PMID 19597337

See all (74) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Recognition of centromere-specific histone Cse4 by the inner kinetochore Okp1-Ame1 complex.
    Title: Recognition of centromere-specific histone Cse4 by the inner kinetochore Okp1-Ame1 complex.
  2. Cdc4 phospho-degrons allow differential regulation of Ame1(CENP-U) protein stability across the cell cycle.
    Title: Cdc4 phospho-degrons allow differential regulation of Ame1(CENP-U) protein stability across the cell cycle.
  3. Findings reveal a new role for the central kinetochore in promoting Sli15 function, and suggest functional Ame1 is required for the correction of defective attachments by promoting the localization of Sli15/INCENP at kinetochores.
    Title: The COMA complex is required for Sli15/INCENP-mediated correction of defective kinetochore attachments.
  4. Ame1 and Okp1 are required for a sustained checkpoint arrest in the presence of mis-segregated chromosomes
    Title: Spindle checkpoint maintenance requires Ame1 and Okp1.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in attachment of spindle microtubules to kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in attachment of spindle microtubules to kinetochore IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
involved_in meiotic cell cycle IEA
Inferred from Electronic Annotation
more info
  
involved_in protein localization to kinetochore IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of COMA complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in chromosome, centromeric region IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm HDA PubMed 
located_in kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
located_in kinetochore IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus HDA PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in spindle pole body HDA PubMed 

General protein information

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Preferred Names
Ame1p
NP_009770.3
  • Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    646158..647132 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178559.3 → NP_009770.3  TPA: Ame1p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009770.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQK7, P38313
    UniProtKB/TrEMBL
    G2W9L4, N1P8P2
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38313.1

Gene LinkOut

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