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MMS4 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852395, updated on 11-Sep-2024

Summary

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Official Symbol
MMS4
Primary source
SGD:S000000302
Locus tag
YBR098W
See related
AllianceGenome:SGD:S000000302; FungiDB:YBR098W; VEuPathDB:YBR098W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
SLX2; YBR100W
Summary
Contributes to crossover junction endodeoxyribonuclease activity. Involved in DNA metabolic process and regulation of reciprocal meiotic recombination. Part of Holliday junction resolvase complex. Orthologous to human EME1 (essential meiotic structure-specific endonuclease 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (441515..443590)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene ubiquitin-binding serine/threonine protein kinase VPS15 Neighboring gene Hsp70 nucleotide exchange factor FES1 Neighboring gene exocyst subunit EXO84

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Chromosome-scale genetic mapping using a set of 16 conditionally stable Saccharomyces cerevisiae chromosomes. Reid RJ, et al. Genetics, 2008 Dec. PMID 18832360, Free PMC Article
  2. Mms4, a putative transcriptional (co)activator, protects Saccharomyces cerevisiae cells from endogenous and environmental DNA damage. Xiao W, et al. Mol Gen Genet, 1998 Apr. PMID 9604884
  3. High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR-Cas9 in yeast. Guo X, et al. Nat Biotechnol, 2018 Jul. PMID 29786095, Free PMC Article
  4. The Mus81-Mms4 structure-selective endonuclease requires nicked DNA junctions to undergo conformational changes and bend its DNA substrates for cleavage. Mukherjee S, et al. Nucleic Acids Res, 2014 Jun. PMID 24744239, Free PMC Article
  5. A junction branch point adjacent to a DNA backbone nick directs substrate cleavage by Saccharomyces cerevisiae Mus81-Mms4. Ehmsen KT, et al. Nucleic Acids Res, 2009 Apr. PMID 19211663, Free PMC Article

See all (144) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The cruciform DNA-binding protein Crp1 stimulates the endonuclease activity of Mus81-Mms4 in Saccharomyces cerevisiae.
    Title: The cruciform DNA-binding protein Crp1 stimulates the endonuclease activity of Mus81-Mms4 in Saccharomyces cerevisiae.
  2. Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
    Title: Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
  3. Study presents evidence that yeast Mus81-Mms4 endonuclease contributes to cell viability in the absence of functional telomerase. This involvement is not through recombination-mediated telomere lengthening as proposed in humans, but rather through an indirect role involving replication stress.
    Title: Saccharomyces cerevisiae Mus81-Mms4 prevents accelerated senescence in telomerase-deficient cells.
  4. These results strongly support the notion that Rad52 and Mus81-Mms4 collaborate and work jointly in processing of homologous recombination intermediates.
    Title: Saccharomyces cerevisiae Mus81-Mms4 and Rad52 can cooperate in the resolution of recombination intermediates.
  5. Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
    Title: Role of PCNA and RFC in promoting Mus81-complex activity.
  6. The scaffold protein Rtt107, which binds the Mus81-Mms4 complex, interacts with Cdc7 and thereby targets Cdc7-Dbf4 and Cdc5 to the complex enabling full Mus81 activation.
    Title: Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis.
  7. Our data suggest that Srs2 and Mus81-Mms4 have critical roles in preventing the formation of (or in resolving) toxic inter-homolog joint molecules, which could otherwise interfere with chromosome segregation and lead to genetic instability.
    Title: Srs2 and Mus81-Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates.
  8. The role of Mms4 in crossover formation during meiotic recombinational repair
    Title: Controlling meiotic recombinational repair - specifying the roles of ZMMs, Sgs1 and Mus81/Mms4 in crossover formation.
  9. Unresolved recombination intermediates are a source of mitotic sister chromatid bridges, and Mus81-Mms4 and Yen1 play a central role in their resolution in vivo.
    Title: Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions.
  10. data point to a coordinated role of Mus81-Mms4 and Srs2 in processing of recombination as well as replication intermediates
    Title: Srs2 promotes Mus81-Mms4-mediated resolution of recombination intermediates.
Submit: New GeneRIF Correction See all GeneRIFs (19)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
contributes_to DNA endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to crossover junction DNA endonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to crossover junction DNA endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nuclease activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA damage response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA topological change IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in double-strand break repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in meiotic cell cycle IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic intra-S DNA damage checkpoint signaling IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in reciprocal meiotic recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in reciprocal meiotic recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of reciprocal meiotic recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in replication fork processing IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in resolution of meiotic recombination intermediates IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in resolution of meiotic recombination intermediates IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
part_of Holliday junction resolvase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of Holliday junction resolvase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of Holliday junction resolvase complex IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Mms4p
NP_009656.2
  • Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    441515..443590
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178446.1 → NP_009656.2  TPA: Mms4p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009656.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQ99, P38257, P38259, Q6B1Z9, Q9URQ2
    UniProtKB/TrEMBL
    D3UEJ3, G2W9A5, N1P9F9
    Conserved Domains (1) summary
    cd20080
    Location:397 → 599
    XPF_nuclease_EME-like; XPF-like nuclease domain of the family of Essential Meiotic Endonucleases (EMEs) and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38257.2

Gene LinkOut

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