GET3 guanine nucleotide exchange factor GET3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GET3
Official Full Name: guanine nucleotide exchange factor GET3
Primary source: SGD:S000002258
Locus tag: YDL100C
See related: AllianceGenome:SGD:S000002258; FungiDB:YDL100C; VEuPathDB:YDL100C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ARR4
Summary: Enables several functions, including ATP hydrolysis activity; guanyl-nucleotide exchange factor activity; and unfolded protein binding activity. Involved in several processes, including establishment of protein localization to membrane; pheromone-dependent signal transduction involved in conjugation with cellular fusion; and retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum. Located in cytosol and endoplasmic reticulum. Part of GET complex. Human ortholog(s) of this gene implicated in dilated cardiomyopathy. Orthologous to human GET3 (guided entry of tail-anchored proteins factor 3, ATPase). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See GET3 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (282112..283176, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

From guide to guard-activation mechanism of the stress-sensing chaperone Get3.
Title: From guide to guard-activation mechanism of the stress-sensing chaperone Get3.
Regulated targeting of the monotopic hairpin membrane protein Erg1 requires the GET pathway.
Title: Regulated targeting of the monotopic hairpin membrane protein Erg1 requires the GET pathway.
GET 1, 2, 3 proteins target MOM proteins to the endoplasmic reticulum.
Title: The GET pathway can increase the risk of mitochondrial outer membrane proteins to be mistargeted to the ER.
Get3 functions as a redox-regulated chaperone, effectively protecting eukaryotic cells against oxidative protein damage.
Title: The protein targeting factor Get3 functions as ATP-independent chaperone under oxidative stress conditions.
The crystal structure of the Get3-Get4-Get5 complex in an ATP-bound state is presented.
Title: Crystal structure of ATP-bound Get3-Get4-Get5 complex reveals regulation of Get3 by Get4.
Get3 moves reversibly to deposition sites for protein aggregates, hence supporting the sequestration of tail-anchored proteins under conditions that prevent tail-anchored protein insertion.
Title: Get3 is a holdase chaperone and moves to deposition sites for aggregated proteins when membrane targeting is blocked.
ATP specifically induces multiple conformational changes in Get3 that culminate in its ATPase activation through tetramerization
Title: Precise timing of ATPase activation drives targeting of tail-anchored proteins.
Get1 uses two interfaces to stabilize the open dimer conformation of Get3.
Title: Get1 stabilizes an open dimer conformation of get3 ATPase by binding two distinct interfaces.
Interaction surface and topology of Get3-Get4-Get5 protein complex, involved in targeting tail-anchored proteins to endoplasmic reticulum
Title: Interaction surface and topology of Get3-Get4-Get5 protein complex, involved in targeting tail-anchored proteins to endoplasmic reticulum.
crystal structures of Get3 in complex with cytosolic domain of Get1 and Get2 in different states; data show Get1 and Get2 can bind simultaneously to Get3;docking to Get1/2 complex allows for conformational change in Get3 required for TA protein insertion
Title: Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein folding chaperone	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to oxidative stress	IDA Inferred from Direct Assay more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-translational protein targeting to endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in protein folding	IEA Inferred from Electronic Annotation more info
involved_in protein insertion into ER membrane	IEA Inferred from Electronic Annotation more info
involved_in response to arsenic-containing substance	IEA Inferred from Electronic Annotation more info
involved_in response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to metal ion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
involved_in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum	IGI Inferred from Genetic Interaction more info	PubMed
involved_in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tail-anchored membrane protein insertion into ER membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in tail-anchored membrane protein insertion into ER membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of GET complex	IBA Inferred from Biological aspect of Ancestor more info
part_of GET complex	IEA Inferred from Electronic Annotation more info
part_of GET complex	IPI Inferred from Physical Interaction more info	PubMed
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	HDA more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: guanine nucleotide exchange factor GET3

NP_010183.1

Guanine nucleotide exchange factor for Gpa1p; amplifies G-protein signaling; subunit of the GET complex involved in ATP-dependent Golgi-to-ER trafficking; binds as a dimer to the transmembrane domain of tail-anchored proteins in the cytosol, shielding them from aqueous solvent until the Get1p/Get2p heterodimer releases the substrate and inserts it into the ER membrane; acts as a chaperone under ATP-depleted oxidative stress conditions; protein abundance increases under DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

282112..283176 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180159.1 → NP_010183.1 TPA: guanine nucleotide exchange factor GET3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010183.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZXM9, B3LGZ3, D6VRQ0, Q12154, Q6B1B6

UniProtKB/TrEMBL

C7GJL6, G2WC64, N1P5N0

Conserved Domains (1) summary

TIGR00345
Location:34 → 334

GET3_arsA_TRC40; transport-energizing ATPase, TRC40/GET3/ArsA family