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GCS1 GTPase-activating protein GCS1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851372, updated on 2-Nov-2024

Summary

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Official Symbol
GCS1
Official Full Name
GTPase-activating protein GCS1
Primary source
SGD:S000002385
Locus tag
YDL226C
See related
AllianceGenome:SGD:S000002385; FungiDB:YDL226C; VEuPathDB:YDL226C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables GTPase activator activity and actin binding activity. Involved in Golgi vesicle transport; actin filament organization; and mitotic cell cycle. Located in cytoskeleton; endosome; and trans-Golgi network. Orthologous to human ARFGAP1 (ADP ribosylation factor GTPase activating protein 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See GCS1 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (51115..52173, complement)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Hsp70 family ATPase SSB1 Neighboring gene Hop Neighboring gene septin SHS1 Neighboring gene Whi4p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A member of a novel family of yeast 'zn-finger' proteins mediates the transition from stationary phase to cell proliferation. Ireland LS, et al. EMBO J, 1994 Aug 15. PMID 8070409, Free PMC Article
  2. Candida albicans AGE3, the ortholog of the S. cerevisiae ARF-GAP-encoding gene GCS1, is required for hyphal growth and drug resistance. Lettner T, et al. PLoS One, 2010 Aug 5. PMID 20700541, Free PMC Article
  3. Expression, analysis, and properties of yeast ADP-ribosylation factor (ARF) GTPase activating proteins (GAPs) Gcs1 and Glo3. Poon PP, et al. Methods Enzymol, 2001. PMID 11210551
  4. Mitochondrial DNA loss by yeast reentry-mutant cells conditionally unable to proliferate from stationary phase. Filipak M, et al. Curr Genet, 1992 Dec. PMID 1473178
  5. A yeast mutant conditionally defective only for reentry into the mitotic cell cycle from stationary phase. Drebot MA, et al. Proc Natl Acad Sci U S A, 1987 Nov. PMID 3317397, Free PMC Article

See all (102) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. ALPS and the predicted AH region are involved in the regulation and function of Gcs1p by interacting with membrane phospholipids and vesicle proteins.
    Title: Identification of a second amphipathic lipid-packing sensor-like motif that contributes to Gcs1p function in the early endosome-to-TGN pathway.
  2. The ArfGAP Gcs1p accelerates vesicle (v)-target membrane (t)-SNARE complex formation in vitro, indicating that ArfGAPs may act as folding chaperones.
    Title: Interaction of SNAREs with ArfGAPs precedes recruitment of Sec18p/NSF.
  3. Taken together, these results indicate that GCS1 is essential for sporulation and suggest that GCS1 positively regulates SPO14.
    Title: The Arf-GTPase-activating protein Gcs1p is essential for sporulation and regulates the phospholipase D Spo14p.
  4. These results suggest that Cdc50p-Drs2p plays an important role in the Arf1p/Gcs1-mediated formation of clathrin-coated vesicles for the retrieval pathway from early endosomes to the trans-Golgi network.
    Title: The functional relationship between the Cdc50p-Drs2p putative aminophospholipid translocase and the Arf GAP Gcs1p in vesicle formation in the retrieval pathway from yeast early endosomes to the TGN.
  5. Release of Arl1p from membranes in cells requires the hydrolysis of bound GTP, which is accelerated by Gcs1p.
    Title: Role for Gcs1p in regulation of Arl1p at trans-Golgi compartments.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables GTPase activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activator activity IEA
Inferred from Electronic Annotation
more info
  
enables actin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in COPI coating of Golgi vesicle IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in Golgi to plasma membrane protein transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin filament organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic cell cycle process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
located_in Golgi membrane IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in cytosol HDA PubMed 
located_in endosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
located_in perinuclear region of cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in trans-Golgi network IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
GTPase-activating protein GCS1
NP_010055.1
  • ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    51115..52173 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180286.1 → NP_010055.1  TPA: GTPase-activating protein GCS1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010055.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VRC9, P35197
    UniProtKB/TrEMBL
    A6ZXA8, C7GSV2, C8Z6P3, G2WBU2, N1P5U7
    Conserved Domains (1) summary
    COG5347
    Location:4 → 350
    COG5347; GTPase-activating protein that regulates ARFs (ADP-ribosylation factors), involved in ARF-mediated vesicular transport [Intracellular trafficking and secretion]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P35197.1

Gene LinkOut

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