GLE1 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: GLE1
Primary source: SGD:S000002366
Locus tag: YDL207W
See related: AllianceGenome:SGD:S000002366; FungiDB:YDL207W; VEuPathDB:YDL207W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BRR3; NLE2; RSS1
Summary: Enables several functions, including inositol hexakisphosphate binding activity; phospholipid binding activity; and translation initiation factor binding activity. Involved in RNA export from nucleus and regulation of translation. Located in cytoplasm. Part of nuclear pore cytoplasmic filaments. Used to study lethal congenital contracture syndrome 1. Human ortholog(s) of this gene implicated in lethal congenital contracture syndrome 1. Orthologous to human GLE1 (GLE1 RNA export mediator). [provided by Alliance of Genome Resources, Apr 2022]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (88248..89864)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and promote Dbp5-mediated tRNA export in vivo in Saccharomyces cerevisiae.
Title: Gle1 is required for tRNA to stimulate Dbp5 ATPase activity in vitro and promote Dbp5-mediated tRNA export in vivo in Saccharomyces cerevisiae.
The nucleoporin Gle1 activates DEAD-box protein 5 (Dbp5) by promoting ATP binding and accelerating rate limiting phosphate release.
Title: The nucleoporin Gle1 activates DEAD-box protein 5 (Dbp5) by promoting ATP binding and accelerating rate limiting phosphate release.
Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation.
Title: Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation.
Gle1 specifically binds IP(6); this interaction is required for the full potentiation of Dbp5 ATPase activity during both mRNA export and translation termination
Title: Control of mRNA export and translation termination by inositol hexakisphosphate requires specific interaction with Gle1.
Study identified several charged surface residues that, when mutated, weaken the binding of Gle1 and inhibit the ability of Gle1 to stimulate Dbp5's ATPase activity.
Title: Structure of the C-terminus of the mRNA export factor Dbp5 reveals the interaction surface for the ATPase activator Gle1.
Study shows that Gle1 and inositol hexakisphosphate are required for efficient translation termination, in addition, Gle1 has a conserved physical association with the initiation factor eIF3.
Title: The mRNA export factor Gle1 and inositol hexakisphosphate regulate distinct stages of translation.
the Gle1-mRNA export pathway is mediated by cytoplasmic inositol hexakisphosphate production
Title: Cytoplasmic inositol hexakisphosphate production is sufficient for mediating the Gle1-mRNA export pathway.
Gle1, Nab2 and Gfd1 associate in a complex;Gfd1 serves as a bridging factor between Gle1 and Nab2-bound mRNA during export
Title: Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function.
Our results define specific functions for Gle1 and InsP6 in mRNA export and suggest that local activation of Dbp5 at the nuclear pore is critical for mRNA export.
Title: Activation of the DExD/H-box protein Dbp5 by the nuclear-pore protein Gle1 and its coactivator InsP6 is required for mRNA export.
Dbp5 activation at NPCs requires Gle1 and InsP6. This would facilitate spatial control of the remodelling of mRNP protein composition during directional transport and provide energy to power transport cycles.
Title: Inositol hexakisphosphate and Gle1 activate the DEAD-box protein Dbp5 for nuclear mRNA export.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables enzyme activator activity	IGI Inferred from Genetic Interaction more info	PubMed
enables enzyme activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables inositol hexakisphosphate binding	IBA Inferred from Biological aspect of Ancestor more info
enables inositol hexakisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phospholipid binding	IBA Inferred from Biological aspect of Ancestor more info
enables phospholipid binding	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor binding	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in mRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA transport	IEA Inferred from Electronic Annotation more info
involved_in poly(A)+ mRNA export from nucleus	IBA Inferred from Biological aspect of Ancestor more info
involved_in poly(A)+ mRNA export from nucleus	IEA Inferred from Electronic Annotation more info
involved_in poly(A)+ mRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translational termination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA export from nucleus	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in nuclear membrane	IEA Inferred from Electronic Annotation more info
part_of nuclear pore	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear pore	IEA Inferred from Electronic Annotation more info
part_of nuclear pore cytoplasmic filaments	IBA Inferred from Biological aspect of Ancestor more info
part_of nuclear pore cytoplasmic filaments	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: nucleoporin GLE1

NP_010074.1

Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

88248..89864

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180267.1 → NP_010074.1 TPA: nucleoporin GLE1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010074.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRE7, Q12315

UniProtKB/TrEMBL

G2WBW0, N1P8A5

Conserved Domains (1) summary

pfam07817
Location:273 → 496

GLE1; GLE1-like protein