RPP0 ribosomal protein P0 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPP0
Official Full Name: ribosomal protein P0
Primary source: SGD:S000004332
Locus tag: YLR340W
See related: AllianceGenome:SGD:S000004332; FungiDB:YLR340W; VEuPathDB:YLR340W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RPL10E
Summary: Enables large ribosomal subunit rRNA binding activity. A structural constituent of ribosome. Involved in cytoplasmic translation and ribosomal large subunit assembly. Located in cytoplasm. Part of cytosolic large ribosomal subunit. Orthologous to several human genes including RPLP0 (ribosomal protein lateral stalk subunit P0). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RPP0 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (805887..806825)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ricin A chain (RTA), which binds to the stalk to depurinate the sarcin/ricin loop, is used as a molecular probe in yeast mutants in which the binding site for one or the other dimer on ribosomal P0 was deleted.
Title: Functional divergence between the two P1-P2 stalk dimers on the ribosome in their interaction with ricin A chain.
The reported results support that while most ribosomes contain a P1alpha/P2beta-P0-P1beta/P2alpha structure in normal conditions, the stalk assembly mechanism can generate alternative compositions, which have been previously detected in the cell.
Title: P1 and P2 protein heterodimer binding to the P0 protein of Saccharomyces cerevisiae is relatively non-specific and a source of ribosomal heterogeneity.
The model of the pentameric ribosomal stalk complex displays three distinct lobes, which may correspond to the individual P1-P2 heterodimers anchored to the C-terminal domain of the P0 protein 60S subunit.
Title: Solution structure of the natively assembled yeast ribosomal stalk determined by small-angle X-ray scattering.
The authors have found that neither P1 nor P2 directly interact with P0 unless the endogenous heterodimer partner is being expressed in the cell.
Title: In vivo formation of a stable pentameric (P2alpha/P1beta)-P0-(P1alpha/P2beta) ribosomal stalk complex in Saccharomyces cerevisiae.
loss of function of ribosomal protein P0 results in a deficit in 60S subunits, which is apparently due to impaired rRNA processing of 27S precursors
Title: Role and dynamics of the ribosomal protein P0 and its related trans-acting factor Mrt4 during ribosome assembly in Saccharomyces cerevisiae.
Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0.
Title: Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0.
results indicate that Mrt4 and P0 bind to the same site in the 25S rRNA.
Title: The amino terminal domain from Mrt4 protein can functionally replace the RNA binding domain of the ribosomal P0 protein.
Two short amino acid regions of P0 protein located at positions 199-230 and 231-258, to be responsible for independent binding of two dimers, P1A-P2B and P1B-P2A respectively.
Title: Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins.
Ribosomal P0 protein participates in ribosomal stalk complex formation through interaction with P1A-P2B complex.
Title: Acquisition of a stable structure by yeast ribosomal P0 protein requires binding of P1A-P2B complex: in vitro formation of the stalk structure.
structure of its 26S rRNA binding site
Title: Characterization of the 26S rRNA-binding domain in Saccharomyces cerevisiae ribosomal stalk phosphoprotein P0.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables large ribosomal subunit rRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables large ribosomal subunit rRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of ribosome	IBA Inferred from Biological aspect of Ancestor more info
enables structural constituent of ribosome	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cytoplasmic translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoplasmic translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosomal large subunit assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of 90S preribosome	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of cytosolic large ribosomal subunit	IBA Inferred from Biological aspect of Ancestor more info
part_of cytosolic large ribosomal subunit	IDA Inferred from Direct Assay more info	PubMed
part_of ribonucleoprotein complex	IEA Inferred from Electronic Annotation more info
located_in ribosome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ribosomal protein P0

NP_013444.1

Conserved ribosomal protein P0 of the ribosomal stalk; involved in interaction between translational elongation factors and the ribosome; phosphorylated on serine 302; homologous to mammalian ribosomal protein LP0 and bacterial L10

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

805887..806825

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182229.1 → NP_013444.1 TPA: ribosomal protein P0 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013444.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYX8, P05317

UniProtKB/TrEMBL

A7A1L9, B3RHL1, C7GUL8, C8ZDU8, G2WJF8, N1NYM9

Conserved Domains (1) summary

cl00376
Location:5 → 312

Ribosomal_L10_P0; Ribosomal protein L10 family; composed of the large subunit ribosomal protein called L10 in bacteria, P0 in eukaryotes, and L10e in archaea, as well as uncharacterized P0-like eukaryotic proteins. In all three kingdoms, L10 forms a tight complex with ...