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CWC24 U2-type spliceosomal complex subunit CWC24 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851033, updated on 2-Nov-2024

Summary

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Official Symbol
CWC24
Official Full Name
U2-type spliceosomal complex subunit CWC24
Primary source
SGD:S000004315
Locus tag
YLR323C
See related
AllianceGenome:SGD:S000004315; FungiDB:YLR323C; VEuPathDB:YLR323C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Predicted to enable DNA binding activity and zinc ion binding activity. Involved in generation of catalytic spliceosome for first transesterification step and snoRNA splicing. Located in nucleus. Part of U2-type spliceosomal complex. Human ortholog(s) of this gene implicated in nonphotosensitive trichothiodystrophy 5. Orthologous to human RNF113A (ring finger protein 113A) and RNF113B (ring finger protein 113B). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See CWC24 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (778173..778952, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Mms22p Neighboring gene Sfh1p Neighboring gene peroxisome biogenesis protein Neighboring gene ribosomal 60S subunit protein L38

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS. Ohrt T, et al. RNA, 2012 Jun. PMID 22535589, Free PMC Article
  2. Functional analysis of Cwc24 ZF-domain in 5' splice site selection. Wu NY, et al. Nucleic Acids Res, 2019 Nov 4. PMID 31504764, Free PMC Article
  3. Cwc24p, a novel Saccharomyces cerevisiae nuclear ring finger protein, affects pre-snoRNA U3 splicing. Goldfeder MB, et al. J Biol Chem, 2008 Feb 1. PMID 17974558
  4. Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts. Coltri PP, et al. PLoS One, 2012. PMID 23029180, Free PMC Article
  5. A protein map of the yeast activated spliceosome as obtained by electron microscopy. Sun C, et al. RNA, 2016 Sep. PMID 27368340, Free PMC Article

See all (22) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Deletion of the ZF domain or mutation of the conserved ZF residues greatly weakened the association of Cwc24 with the spliceosome, and lowered the affinity and specificity of its interaction with the 5' splice site, resulting in atypical interactions of U5, U6 and Prp8 with the 5' splice site, and aberrant cleavage at the 5' splice site
    Title: Functional analysis of Cwc24 ZF-domain in 5' splice site selection.
  2. propose a model in which Cwc24p is required for stabilizing the U2 association with primary transcripts, and therefore, especially important for splicing of RNAs containing non-consensus branchpoint sequences
    Title: Cwc24p is a general Saccharomyces cerevisiae splicing factor required for the stable U2 snRNP binding to primary transcripts.
  3. Cwc24p localizes to the cell nucleus, and its depletion leads to the accumulation of both U3 pre-snoRNAs.
    Title: Cwc24p, a novel Saccharomyces cerevisiae nuclear ring finger protein, affects pre-snoRNA U3 splicing.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in generation of catalytic spliceosome for first transesterification step IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA splicing, via spliceosome IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in snoRNA splicing IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in snoRNA splicing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of U2-type spliceosomal complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of U2-type spliceosomal complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of spliceosomal complex IEA
Inferred from Electronic Annotation
more info
  
part_of spliceosomal complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
U2-type spliceosomal complex subunit CWC24
NP_013427.1
  • General splicing factor; required for stable U2 snRNP binding to primary transcripts; essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; similar to S. pombe Cwf24p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    778173..778952 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182212.1 → NP_013427.1  TPA: U2-type spliceosomal complex subunit CWC24 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013427.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYW5, P53769
    UniProtKB/TrEMBL
    A7A1K6, B3RHJ8, B5VNL8, C8ZDT4, G2WJE4, N1P745
    Conserved Domains (1) summary
    COG5152
    Location:1 → 259
    COG5152; Uncharacterized conserved protein, contains RING and CCCH-type Zn-fingers [General function prediction only]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P53769.1

Gene LinkOut

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