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ECM22 Ecm22p [ Saccharomyces cerevisiae S288C ]

Gene ID: 850928, updated on 2-Nov-2024

Summary

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Official Symbol
ECM22
Official Full Name
Ecm22p
Primary source
SGD:S000004218
Locus tag
YLR228C
See related
AllianceGenome:SGD:S000004218; FungiDB:YLR228C; VEuPathDB:YLR228C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in positive regulation of biosynthetic process; positive regulation of filamentous growth of a population of unicellular organisms in response to starvation; and positive regulation of sterol import. Located in membrane and perinuclear region of cytoplasm. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See ECM22 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (600019..602463, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene tRNA-Leu Neighboring gene gag protein Neighboring gene gag-pol fusion protein Neighboring gene Rho family GTPase CDC42 Neighboring gene tRNA-Ile

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. UPC2A is required for high-level azole antifungal resistance in Candida glabrata. Whaley SG, et al. Antimicrob Agents Chemother, 2014 Aug. PMID 24867980, Free PMC Article
  2. Transcriptional regulation of ergosterol biosynthesis genes in response to iron deficiency. Jordá T, et al. Environ Microbiol, 2022 Nov. PMID 36382795, Free PMC Article
  3. Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae. Marie C, et al. Fungal Genet Biol, 2008 Oct. PMID 18675371, Free PMC Article
  4. Identification of a UPC2 homolog in Saccharomyces cerevisiae and its involvement in aerobic sterol uptake. Shianna KV, et al. J Bacteriol, 2001 Feb. PMID 11208779, Free PMC Article
  5. Structural mechanism of ergosterol regulation by fungal sterol transcription factor Upc2. Yang H, et al. Nat Commun, 2015 Feb 6. PMID 25655993

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Cells lacking both ECM22 and UPC2 display strong mating defects whereas deletion of either gene has no effect. Ecm22 and Upc2 positively regulate basal expression of PRM1 and PRM4. These genes are strongly induced in response to mating pheromone, which is also largely dependent on ECM22 and UPC2.
    Title: Ecm22 and Upc2 regulate yeast mating through control of expression of the mating genes PRM1 and PRM4.
  2. Overexpression of either ECM22 or UPC2 leads to increased filamentation.
    Title: The zinc cluster proteins Upc2 and Ecm22 promote filamentation in Saccharomyces cerevisiae by sterol biosynthesis-dependent and -independent pathways.
  3. Perinuclear localization of Upc2p-GFP and Ecm22p-GFP was increased when ergosterol biosynthesis was blocked by azole drug treatment.
    Title: Cytoplasmic localization of sterol transcription factors Upc2p and Ecm22p in S. cerevisiae.
  4. Upc2p and Ecm22p differ in their contributions to the regulation of their targets
    Title: Dual activators of the sterol biosynthetic pathway of Saccharomyces cerevisiae: similar activation/regulatory domains but different response mechanisms.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription activator activity, RNA polymerase II-specific IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-binding transcription activator activity, RNA polymerase II-specific IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables DNA-binding transcription factor activity, RNA polymerase II-specific IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-binding transcription factor activity, RNA polymerase II-specific IEA
Inferred from Electronic Annotation
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables sequence-specific DNA binding HDA PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in positive regulation of ergosterol biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of filamentous growth of a population of unicellular organisms in response to starvation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of filamentous growth of a population of unicellular organisms in response to starvation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of sterol import IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in perinuclear region of cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
Ecm22p
NP_013329.1
  • Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes upon sterol depletion, after relocating from intracellular membranes to perinuclear foci; redundant activator of filamentation with UPC2, up-regulating the expression of genes involved in filamentous growth; contains Zn[2]-Cys[6] binuclear cluster; ECM22 has a paralog, UPC2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    600019..602463 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182115.1 → NP_013329.1  TPA: Ecm22p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013329.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYN0, Q05958
    UniProtKB/TrEMBL
    B3RHB6, B5VNE5, C7GT26, C8ZDJ2, N1P1D4
    Conserved Domains (2) summary
    smart00066
    Location:39 → 80
    GAL4; GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain
    cl23766
    Location:508 → 638
    fungal_TF_MHR; fungal transcription factor regulatory middle homology region
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q05958.1

Gene LinkOut

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