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EMG1 18S rRNA pseudouridine methyltransferase [ Saccharomyces cerevisiae S288C ]

Gene ID: 850883, updated on 2-Nov-2024

Summary

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Official Symbol
EMG1
Official Full Name
18S rRNA pseudouridine methyltransferase
Primary source
SGD:S000004176
Locus tag
YLR186W
See related
AllianceGenome:SGD:S000004176; FungiDB:YLR186W; VEuPathDB:YLR186W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
NEP1
Summary
Enables rRNA (pseudouridine) methyltransferase activity. Involved in rRNA processing. Located in cytoplasm and nucleus. Part of small-subunit processome. Used to study Bowen-Conradi syndrome. Human ortholog(s) of this gene implicated in Bowen-Conradi syndrome. Orthologous to human EMG1 (EMG1 N1-specific pseudouridine methyltransferase). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See EMG1 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (523632..524390)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Tos4p Neighboring gene ribosomal 60S subunit protein L37A Neighboring gene Skg3p Neighboring gene ATP-binding cassette permease MDL1

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases. Leulliot N, et al. Nucleic Acids Res, 2008 Feb. PMID 18063569, Free PMC Article
  2. The eukaryote-specific N-terminal extension of ribosomal protein S31 contributes to the assembly and function of 40S ribosomal subunits. Fernández-Pevida A, et al. Nucleic Acids Res, 2016 Sep 19. PMID 27422873, Free PMC Article
  3. The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site. Taylor AB, et al. Nucleic Acids Res, 2008 Mar. PMID 18208838, Free PMC Article
  4. Mutation of a gene essential for ribosome biogenesis, EMG1, causes Bowen-Conradi syndrome. Armistead J, et al. Am J Hum Genet, 2009 Jun. PMID 19463982, Free PMC Article
  5. Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis. Eschrich D, et al. Curr Genet, 2002 Feb. PMID 11935223

See all (76) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Utp30 and Nep1 act together during pre-ribosomal complex formation.
    Title: Genetic interactions of yeast NEP1 (EMG1), encoding an essential factor in ribosome biogenesis.
  2. snR57 and Nep1 participate in a pre-rRNA conformational switch that allows the proper assembly of 40S ribosomal protein S19.
    Title: Nop6, a component of 90S pre-ribosomal particles, is required for 40S ribosomal subunit biogenesis in Saccharomyces cerevisiae.
  3. the identification of tma23 and nop6 mutations as recessive suppressors of a nep1(ts) mutant allele and the nep1 deletion as well
    Title: Mutations in the nucleolar proteins Tma23 and Nop6 suppress the malfunction of the Nep1 protein.
  4. Emg1 represents a new structural class in the superfamily of alpha/beta knot fold methyltransferases.
    Title: The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases.
  5. These results suggest that Nep1p binds adjacent to helix 47 of the 18S rRNA and possibly supports the association of Rps19p to pre-ribosomal particles.
    Title: Genetic evidence for 18S rRNA binding and an Rps19p assembly function of yeast nucleolar protein Nep1p.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (pseudouridine) methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA (pseudouridine) methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (pseudouridine) methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables rRNA (pseudouridine) methyltransferase activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables rRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA base methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in rRNA base methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA base methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA base methylation ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosomal small subunit biogenesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in ribosomal small subunit biogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of 90S preribosome HDA PubMed 
located_in cytoplasm HDA PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear microtubule IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear periphery IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
  
part_of small-subunit processome IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of small-subunit processome IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
18S rRNA pseudouridine methyltransferase
NP_013287.1
  • Methyltransferase for rRNA; methylates pseudouridine 18S rRNA residue 1191; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independent of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and equivalent yeast mutation affects Emg1p dimerization and localization but not methyltransferase activity; human EMG1 complements lethality of null and ts mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    523632..524390
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182073.1 → NP_013287.1  TPA: 18S rRNA pseudouridine methyltransferase [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013287.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYI9, E9P8U2, Q06287
    UniProtKB/TrEMBL
    A7A176, B3RH77, B5VNA4, C7GIX3, C8ZDF7, G2WJ18, N1P6S0
    Conserved Domains (1) summary
    cd18088
    Location:41 → 246
    Nep1-like; 18S rRNA (pseudouridine(1248)-N1)-methyltransferase Nep1
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q06287.1

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