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SFI1 Sfi1p [ Saccharomyces cerevisiae S288C ]

Gene ID: 850657, updated on 2-Nov-2024

Summary

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Official Symbol
SFI1
Official Full Name
Sfi1p
Primary source
SGD:S000003926
Locus tag
YLL003W
See related
AllianceGenome:SGD:S000003926; FungiDB:YLL003W; VEuPathDB:YLL003W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Involved in G2/M transition of mitotic cell cycle; spindle assembly; and spindle pole body duplication. Located in half bridge of spindle pole body. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SFI1 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (143201..146041)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Spo75p Neighboring gene origin recognition complex subunit 3 Neighboring gene H3 histone acetyltransferase RTT109 Neighboring gene dynamin-related GTPase DNM1

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Deletion of SFI1, a novel suppressor of partial Ras-cAMP pathway deficiency in the yeast Saccharomyces cerevisiae, causes G(2) arrest. Ma P, et al. Yeast, 1999 Aug. PMID 10455233
  2. Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication. Li S, et al. J Cell Biol, 2006 Jun 19. PMID 16785321, Free PMC Article
  3. New insights into the interaction of centrin with Sfi1. Martinez-Sanz J, et al. Biochim Biophys Acta, 2016 Apr. PMID 26779587
  4. Licensing of yeast centrosome duplication requires phosphoregulation of sfi1. Avena JS, et al. PLoS Genet, 2014 Oct. PMID 25340401, Free PMC Article
  5. The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body. Rüthnick D, et al. J Cell Biol, 2021 Mar 1. PMID 33523111, Free PMC Article

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Kar1 binding is restricted to Sfi1-C-terminal and Sfi1 C-terminal centrin-binding repeats, and centrin and Kar1 provide cross-links, while Sfi1-C-terminal stabilizes the bridge and ensures timely spindle pole body separation.
    Title: Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope.
  2. Our findings lead to a model whereby phosphoregulation of Sfi1 by Cdk1 has the dual function of promoting spindle pole bodies separation for spindle formation and preventing premature SPB duplication.
    Title: Licensing of yeast centrosome duplication requires phosphoregulation of sfi1.
  3. After spindle pole body duplication, the dimerized Sfi1 is severed to allow spindle formation and spindle pole body reduplication.
    Title: Molecular mechanisms that restrict yeast centrosome duplication to one event per cell cycle.
  4. Results describe additional functions for Sfi1p in bipolar spindle assembly and function based on the actions of several novel sfi1 alleles.
    Title: Novel sfi1 alleles uncover additional functions for Sfi1p in bipolar spindle assembly and function.
  5. Sfi1 bound with Cdc31 in a complex have a role in spindle pole body duplication.
    Title: Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in G2/M transition of mitotic cell cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
involved_in spindle assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in spindle pole body duplication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in half bridge of spindle pole body IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle pole IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Sfi1p
NP_013098.1
  • Centrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    143201..146041
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181823.1 → NP_013098.1  TPA: Sfi1p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013098.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VY00, Q12369
    UniProtKB/TrEMBL
    B3LTG3, C7GPG0
    Conserved Domains (2) summary
    pfam08457
    Location:259 → 794
    Sfi1; Sfi1 spindle body protein
    pfam10638
    Location:812 → 913
    Sfi1_C; Spindle body associated protein C-terminus
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q12369.1

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