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SAD1 mRNA splicing protein SAD1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 850555, updated on 2-Nov-2024

Summary

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Official Symbol
SAD1
Official Full Name
mRNA splicing protein SAD1
Primary source
SGD:S000001901
Locus tag
YFR005C
See related
AllianceGenome:SGD:S000001901; FungiDB:YFR005C; VEuPathDB:YFR005C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Predicted to enable cysteine-type deubiquitinase activity and zinc ion binding activity. Involved in spliceosomal complex assembly. Located in nucleus. Orthologous to human USP39 (ubiquitin specific peptidase 39). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SAD1 in Genome Data Viewer
Location:
chromosome: VI
Exon count:
1
Sequence:
Chromosome: VI; NC_001138.5 (154527..155873, complement)

Chromosome VI - NC_001138.5Genomic Context describing neighboring genes Neighboring gene type 1 protein phosphatase-activating protein YPI1 Neighboring gene proteasome regulatory particle lid subunit RPN11 Neighboring gene putative Xaa-Pro dipeptidase Neighboring gene tRNA-Phe

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre-mRNA splicing. Lygerou Z, et al. Mol Cell Biol, 1999 Mar. PMID 10022888, Free PMC Article
  2. Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3. Bupp JM, et al. J Cell Biol, 2007 Dec 3. PMID 18039933, Free PMC Article
  3. The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing. Hadjivassiliou H, et al. RNA, 2014 May. PMID 24681967, Free PMC Article
  4. The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. Allen JB, et al. Genes Dev, 1994 Oct 15. PMID 7958905
  5. An Allosteric Network for Spliceosome Activation Revealed by High-Throughput Suppressor Analysis in Saccharomyces cerevisiae. Brow DA. Genetics, 2019 May. PMID 30898770, Free PMC Article

See all (35) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. we propose that Sad1's ZnF-UBP acts in a ubiquitin-independent capacity to recruit and/or activate Sad1's iUSP domain to interact with the spliceosome
    Title: The crystal structure of S. cerevisiae Sad1, a catalytically inactive deubiquitinase that is broadly required for pre-mRNA splicing.
  2. Sad1 maintains the integrity of the tri-snRNP by counteracting Brr2-mediated dissociation of tri-snRNP.
    Title: Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasis.
  3. A previously unrecognized function for the Saccharomyces cerevisiae Sad1-UNC-84 domain protein Mps3 in regulating telomere positioning in mitotic cells, is reported.
    Title: Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables cysteine-type deubiquitinase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA splicing, via spliceosome IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein deubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in spliceosomal complex assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in spliceosomal complex assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in spliceosomal complex assembly IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of spliceosomal complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
mRNA splicing protein SAD1
NP_116660.1
  • Conserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly all intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001138.5 Reference assembly

    Range
    154527..155873 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179970.1 → NP_116660.1  TPA: mRNA splicing protein SAD1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_116660.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VTN5, P43589
    UniProtKB/TrEMBL
    A7A243, G2WDE4, N1P378
    Conserved Domains (1) summary
    cd02669
    Location:30 → 445
    Peptidase_C19M; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P43589.1

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