MSH4 MutS family protein MSH4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MSH4
Official Full Name: MutS family protein MSH4
Primary source: SGD:S000001891
Locus tag: YFL003C
See related: AllianceGenome:SGD:S000001891; FungiDB:YFL003C; VEuPathDB:YFL003C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to DNA binding activity. Involved in reciprocal meiotic recombination. Located in nuclear chromosome. Part of MutSgamma complex. Human ortholog(s) of this gene implicated in primary ovarian insufficiency and spermatogenic failure 2. Orthologous to human MSH4 (mutS homolog 4). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See MSH4 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (134521..137157, complement)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulation of Msh4-Msh5 association with meiotic chromosomes in budding yeast.
Title: Regulation of Msh4-Msh5 association with meiotic chromosomes in budding yeast.
Msh4 is identified as an intrinsically unstable protein that is directly targeted for proteasomal degradation by an N-terminal degron, thereby rendering MutSgamma inactive for its crossover functions. Activation occurs by neutralizing the Msh4 degron via phosphorylation. Thus, a key meiotic pro-crossover factor is activated by attenuating its proteolysis.
Title: Regulated Proteolysis of MutSγ Controls Meiotic Crossing Over.
We closely examined two candidate genes, the chromatin remodeling gene IOC4 and the meiosis-related gene MSH4, which when knocked-out resulted in gene expression alterations affecting genes involved in cell division and chromosome organization, as well as DNA repair and recombination, respectively.
Title: Systematic Identification of Determinants for Single-Strand Annealing-Mediated Deletion Formation in Saccharomyces cerevisiae.
The excess crossovers observed in synaptonemal complex (SC), central element-deficient mutants are Msh4, Zip1, and Zip4 dependent, clearly indicating the existence of two classes of SC proteins-a class with procrossover function(s) that are also necessary for SC assembly and a class that is not required for crossover formation but essential for SC assembly
Title: Synaptonemal Complex Proteins of Budding Yeast Define Reciprocal Roles in MutSγ-Mediated Crossover Formation.
a structural rationale for the meiotic defects observed in the msh4/5 point mutations. The mutations are likely to affect stability of the Msh4/5 proteins and/or interactions with DNA.
Title: Structural insights into Saccharomyces cerevisiae Msh4-Msh5 complex function using homology modeling.
Msh4 and msh5 mutants displayed reduced crossing over in meiosis but maintained wild-type spore viability.
Title: Genetic analysis of baker's yeast Msh4-Msh5 reveals a threshold crossover level for meiotic viability.
analysis of temperature-dependent modulation of chromosome segregation in msh4 mutants of budding yeast
Title: Temperature-dependent modulation of chromosome segregation in msh4 mutants of budding yeast.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent DNA damage sensor activity	IEA Inferred from Electronic Annotation more info
contributes_to D-loop DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to Y-form DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to four-way junction DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mismatched DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in reciprocal meiotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of MutSgamma complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: MutS family protein MSH4

NP_116652.1

Protein involved in meiotic recombination; required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001138.5 Reference assembly

Range

134521..137157 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179963.1 → NP_116652.1 TPA: MutS family protein MSH4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_116652.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTM6, P40965

UniProtKB/TrEMBL

A7A235, N1P4Y7

Conserved Domains (1) summary

COG0249
Location:1 → 870

MutS; DNA mismatch repair ATPase MutS [Replication, recombination and repair]