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LDB16 Ldb16p [ Saccharomyces cerevisiae S288C ]

Gene ID: 850351, updated on 2-Nov-2024

Summary

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Official Symbol
LDB16
Official Full Name
Ldb16p
Primary source
SGD:S000000511
Locus tag
YCL005W
See related
AllianceGenome:SGD:S000000511; FungiDB:YCL005W; VEuPathDB:YCL005W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Involved in lipid droplet organization and negative regulation of sphingolipid biosynthetic process. Located in endoplasmic reticulum membrane. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See LDB16 in Genome Data Viewer
Location:
chromosome: III
Exon count:
1
Sequence:
Chromosome: III; NC_001135.5 (108021..108791)

Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene H(+)-transporting V0 sector ATPase subunit e Neighboring gene ncRNA Neighboring gene CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters. Renne MF, et al. J Cell Biol, 2022 Sep 5. PMID 35938957, Free PMC Article
  2. Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex. Klug YA, et al. Nat Commun, 2021 Oct 8. PMID 34625558, Free PMC Article
  3. Seipin negatively regulates sphingolipid production at the ER-LD contact site. Su WC, et al. J Cell Biol, 2019 Nov 4. PMID 31594806, Free PMC Article
  4. A genome-wide screen for Saccharomyces cerevisiae nonessential genes involved in mannosyl phosphate transfer to mannoprotein-linked oligosaccharides. Corbacho I, et al. Fungal Genet Biol, 2005 Sep. PMID 15993632
  5. The seipin complex Fld1/Ldb16 stabilizes ER-lipid droplet contact sites. Grippa A, et al. J Cell Biol, 2015 Nov 23. PMID 26572621, Free PMC Article

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters.
    Title: Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters.
  2. Proper droplet formation requires the collaboration of Sei1p and Ldb16.
    Title: Dissecting seipin function: the localized accumulation of phosphatidic acid at ER/LD junctions in the absence of seipin is suppressed by Sei1p(ΔNterm) only in combination with Ldb16p.
  3. The Fld1/Ldb16 complex facilitates the establishment of lipid droplet (LD) identity by acting as a diffusion barrier at the endoplasmic reticulum-LD contact sites.
    Title: The seipin complex Fld1/Ldb16 stabilizes ER-lipid droplet contact sites.
  4. Fld1(seipin)-Ldb16 complex affects phosphatidic acid homeostasis at an lipid droplets-forming subdomain of the nuclear envelope.
    Title: Seipin is involved in the regulation of phosphatidic acid metabolism at a subdomain of the nuclear envelope in yeast.
  5. The stability of Ldb16 requires Fld1, as Ldb16 is subjected to ER-associated degradation (ERAD) in the absence of Fld1 but is stabilized when Fld1 is present.
    Title: Control of lipid droplet size in budding yeast requires the collaboration between Fld1 and Ldb16.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in lipid droplet organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of sphingolipid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in endoplasmic reticulum membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in lipid droplet HDA PubMed 
located_in lipid droplet IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion HDA PubMed 

General protein information

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Preferred Names
Ldb16p
NP_009922.2
  • Protein involved in lipid droplet (LD) assembly; forms a complex with Sei1p at ER-LD contact sites, stabilizing contact sites; ensures that LDs bud from the ER towards the cytosolic side of the membrane; null mutants have decreased net negative cell surface charge and localized accumulation of phosphatidic acid (PA) marker proteins; GFP-fusion protein expression is induced in response to MMS; null mutant can be complemented by the human seipin, BSCL2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001135.5 Reference assembly

    Range
    108021..108791
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178654.1 → NP_009922.2  TPA: Ldb16p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009922.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VR06, P25587, Q8NIM5
    UniProtKB/TrEMBL
    A6ZTH8, B3LU69, B5VEU6, C7GQL1, C8Z480, G2WA14, N1P994
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25587.2

Gene LinkOut

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