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HCS1 holocarboxylase synthase 1 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 817112, updated on 18-Sep-2024

Summary

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Official Symbol
HCS1
Official Full Name
holocarboxylase synthase 1
Primary source
TAIR:AT2G25710
Locus tag
AT2G25710
See related
Araport:AT2G25710
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
F3N11.16; F3N11_16; HOLOCARBOXYLASE SYNTHASE; holocarboxylase synthase 1
Summary
Encodes a dual-targeted biotin holocarboxylase synthetase that can localize to the chloroplast and the cytosol. In vitro, it has been shown to catalyze the addition of biotin to the BCCP subunit of acetyl-CoA carboxylase and it can also biotinylate methylcrotonyl-CoA carboxylase. A small upstream ORF in the 5'UTR (uORF24) regulates the differential targeting of this enzyme.
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Genomic context

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See HCS1 in Genome Data Viewer
Location:
chromosome: 2
Exon count:
10
Sequence:
Chromosome: 2; NC_003071.7 (10952547..10955411)

Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene DUF581 family protein, putative (DUF581) Neighboring gene SKP1-like 3 Neighboring gene uncharacterized protein Neighboring gene zinc finger FYVE domain protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Purification and properties of the chloroplastic form of biotin holocarboxylase synthetase from Arabidopsis thaliana overexpressed in Escherichia coli. Tissot G, et al. Eur J Biochem, 1998 Dec 1. PMID 9874227
  2. Dual targeting of Arabidopsis holocarboxylase synthetase1: a small upstream open reading frame regulates translation initiation and protein targeting. Puyaubert J, et al. Plant Physiol, 2008 Feb. PMID 18156294, Free PMC Article
  3. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana. Lin X, et al. Nature, 1999 Dec 16. PMID 10617197

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. These findings strongly suggest that HCS1 is the only protein responsible for HCS activity in Arabidopsis cells, including the cytosolic, mitochondrial, and plastidial compartments.
    Title: Dual targeting of Arabidopsis holocarboxylase synthetase1: a small upstream open reading frame regulates translation initiation and protein targeting.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Homology

NM_001336012.1
NM_001336013.1
NM_001336014.1
NM_001336015.1
NM_001336016.1
NM_128130.3
NM_179736.1

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables biotin--[biotin carboxyl-carrier protein] ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables biotin--[biotin carboxyl-carrier protein] ligase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within biotin carboxyl carrier protein biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein modification process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in chloroplast IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chloroplast ISM
Inferred from Sequence Model
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plastid IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
holocarboxylase synthase 1
NP_565605.1
  • holocarboxylase synthase 1 (HCS1); FUNCTIONS IN: biotin-[acetyl-CoA-carboxylase] ligase activity, catalytic activity; INVOLVED IN: protein modification process; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Biotin protein ligase, C-terminal (InterPro:IPR003142), Biotin/lipoate A/B protein ligase (InterPro:IPR004143), Biotin--acetyl-CoA-carboxylase ligase (InterPro:IPR004408); BEST Arabidopsis thaliana protein match is: holocarboxylase synthetase 2 (TAIR:AT1G37150.2); Has 6062 Blast hits to 6058 proteins in 2314 species: Archae - 226; Bacteria - 4056; Metazoa - 119; Fungi - 135; Plants - 62; Viruses - 0; Other Eukaryotes - 1464 (source: NCBI BLink).
NP_850067.1
  • holocarboxylase synthase 1 (HCS1); FUNCTIONS IN: biotin-[acetyl-CoA-carboxylase] ligase activity, catalytic activity; INVOLVED IN: protein modification process; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Biotin protein ligase, C-terminal (InterPro:IPR003142), Biotin/lipoate A/B protein ligase (InterPro:IPR004143), Biotin--acetyl-CoA-carboxylase ligase (InterPro:IPR004408); BEST Arabidopsis thaliana protein match is: holocarboxylase synthetase 2 (TAIR:AT1G37150.2); Has 6039 Blast hits to 6035 proteins in 2305 species: Archae - 225; Bacteria - 4034; Metazoa - 119; Fungi - 135; Plants - 62; Viruses - 0; Other Eukaryotes - 1464 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003071.7 Reference assembly

    Range
    10952547..10955411
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001336011.1 → NP_001324826.1  holocarboxylase synthase 1 [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VQX4, A0A1P8B1A6

  2. NM_128130.3 → NP_565605.1  holocarboxylase synthase 1 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_565605.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P92975, Q8S4V8, Q9SL92
    UniProtKB/TrEMBL
    A0A5S9X1E4
    Conserved Domains (2) summary
    cd16442
    Location:113 → 288
    BPL; biotin protein ligase
    pfam02237
    Location:306 → 360
    BPL_C; Biotin protein ligase C terminal domain

  3. NM_001336012.1 → NP_001324829.1  holocarboxylase synthase 1 [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VMU1, A0A5S9X1E4
    Conserved Domains (2) summary
    cd16442
    Location:75 → 250
    BPL; biotin protein ligase
    pfam02237
    Location:268 → 323
    BPL_C; Biotin protein ligase C terminal domain

  4. NM_001336013.1 → NP_001324830.1  holocarboxylase synthase 1 [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VMU1, A0A5S9X1E4
    Conserved Domains (2) summary
    cd16442
    Location:75 → 250
    BPL; biotin protein ligase
    pfam02237
    Location:268 → 323
    BPL_C; Biotin protein ligase C terminal domain

  5. NM_001336014.1 → NP_001324827.1  holocarboxylase synthase 1 [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VQX4, A0A1P8B1A4

  6. NM_001336015.1 → NP_001324828.1  holocarboxylase synthase 1 [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VQX4, A0A1P8B1A4

  7. NM_001336016.1 → NP_001324831.1  holocarboxylase synthase 1 [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VQX4, A0A1P8B1A6

  8. NM_179736.1 → NP_850067.1  holocarboxylase synthase 1 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_850067.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P92975, Q8S4V8, Q9SL92
    UniProtKB/TrEMBL
    A0A5S9X1E4
    Conserved Domains (2) summary
    cd16442
    Location:113 → 288
    BPL; biotin protein ligase
    pfam02237
    Location:306 → 360
    BPL_C; Biotin protein ligase C terminal domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9SL92.2

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