Ago4 argonaute RISC catalytic subunit 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ago4provided by MGI
Official Full Name: argonaute RISC catalytic subunit 4provided by MGI
Primary source: MGI:MGI:1924100
See related: Ensembl:ENSMUSG00000042500 AllianceGenome:MGI:1924100
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Eif2c4; 5730550L01Rik
Summary: Enables RNA binding activity. Acts upstream of or within several processes, including male gonad development; meiotic nuclear division; and miRNA metabolic process. Located in P-body and nucleus. Part of RISC complex. Is expressed in several structures, including central nervous system; lung; sensory organ; tooth; and vibrissa. Orthologous to human AGO4 (argonaute RISC component 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in whole brain E14.5 (RPKM 3.1), CNS E18 (RPKM 2.7) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 4 D2.2; 4 60.9 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	4	NC_000070.7 (126383280..126427379, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	4	NC_000070.6 (126489487..126533586, complement)

Chromosome 4 - NC_000070.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

up-regulated in murine macrophage RAW264.7 cells transfected by Echinococcus multilocularis miR-71
Title: Effects of Echinococcus multilocularis miR-71 mimics on murine macrophage RAW264.7 cells.
show that Ago4(-/-) spermatogonia initiate meiosis early, resulting from premature induction of retinoic acid-response genes
Title: AGO4 regulates entry into meiosis and influences silencing of sex chromosomes in the male mouse germline.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Eif2c4 (e-PCR), detects polymorphism
- UniSTS:471829

Eif2c4 (e-PCR), detects polymorphism
- UniSTS:478997

NoName (e-PCR)
- UniSTS:235029

AI481660 (e-PCR)
- UniSTS:165355

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	ISO Inferred from Sequence Orthology more info
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables miRNA binding	IEA Inferred from Electronic Annotation more info
enables miRNA binding	ISO Inferred from Sequence Orthology more info
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IEA Inferred from Electronic Annotation more info
enables single-stranded RNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IEA Inferred from Electronic Annotation more info
involved_in RISC complex assembly	ISO Inferred from Sequence Orthology more info
involved_in RNA secondary structure unwinding	IEA Inferred from Electronic Annotation more info
involved_in RNA secondary structure unwinding	ISO Inferred from Sequence Orthology more info
involved_in mRNA catabolic process	ISO Inferred from Sequence Orthology more info
involved_in mRNA catabolic process	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within male gonad development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within miRNA metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA processing	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by inhibition of translation	ISO Inferred from Sequence Orthology more info
involved_in miRNA-mediated gene silencing by inhibition of translation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in pre-miRNA processing	IEA Inferred from Electronic Annotation more info
involved_in pre-miRNA processing	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within synaptonemal complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	ISO Inferred from Sequence Orthology more info
part_of RISC-loading complex	IEA Inferred from Electronic Annotation more info
part_of RISC-loading complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein argonaute-4

Names: Piwi/Argonaute family protein meIF2C4; argonaute 4; argonaute RISC catalytic component 4; eukaryotic translation initiation factor 2C, 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_153177.3 → NP_694817.2 protein argonaute-4

See identical proteins and their annotated locations for NP_694817.2

Status: VALIDATED

Source sequence(s)

AI481660, AK017827, AV306945, BB616446, BC096023

Consensus CDS

CCDS18654.1

UniProtKB/Swiss-Prot

Q4VBD7, Q6ZPM6, Q8BTF4, Q8CJF8

Related

ENSMUSP00000081312.5, ENSMUST00000084289.5

Conserved Domains (5) summary

PLN03202
Location:15 → 860

PLN03202; protein argonaute; Provisional

cd02846
Location:217 → 337

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:384 → 819

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:167 → 216

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:18 → 155

ArgoN; N-terminal domain of argonaute

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000070.7 Reference GRCm39 C57BL/6J

Range

126383280..126427379 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006503481.5 → XP_006503544.1 protein argonaute-4 isoform X2

Conserved Domains (2) summary

PLN03202
Location:15 → 718

PLN03202; protein argonaute; Provisional

cd04657
Location:384 → 739

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
XM_036164538.1 → XP_036020431.1 protein argonaute-4 isoform X5

UniProtKB/TrEMBL

Q8BTF2

Conserved Domains (1) summary

cd04657
Location:5 → 363

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
XM_006503483.5 → XP_006503546.1 protein argonaute-4 isoform X4

Conserved Domains (2) summary

cd04657
Location:91 → 526

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam02170
Location:2 → 61

PAZ; PAZ domain
XM_036164539.1 → XP_036020432.1 protein argonaute-4 isoform X5

UniProtKB/TrEMBL

Q8BTF2

Conserved Domains (1) summary

cd04657
Location:5 → 363

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...
XM_036164537.1 → XP_036020430.1 protein argonaute-4 isoform X1

Conserved Domains (4) summary

cd02846
Location:217 → 337

PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...

cd04657
Location:384 → 759

Piwi_ago-like; PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the ...

pfam08699
Location:166 → 216

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:26 → 155

ArgoN; N-terminal domain of argonaute
XM_006503482.4 → XP_006503545.1 protein argonaute-4 isoform X3

Conserved Domains (2) summary

PLN03202
Location:15 → 682

PLN03202; protein argonaute; Provisional

cl00628
Location:384 → 682

Piwi-like; PIWI domain. Domain found in proteins involved in RNA silencing. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The central component ...