U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

PPP2R5A protein phosphatase 2 regulatory subunit B'alpha [ Homo sapiens (human) ]

Gene ID: 5525, updated on 14-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
PPP2R5Aprovided by HGNC
Official Full Name
protein phosphatase 2 regulatory subunit B'alphaprovided by HGNC
Primary source
HGNC:HGNC:9309
See related
Ensembl:ENSG00000066027 MIM:601643; AllianceGenome:HGNC:9309
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
B56A; PR61A; B56alpha
Summary
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
Expression
Ubiquitous expression in fat (RPKM 41.9), lung (RPKM 33.2) and 25 other tissues See more
Orthologs
mouse all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See PPP2R5A in Genome Data Viewer
Location:
1q32.3
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (212285410..212361853)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (211531574..211606538)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (212458752..212535195)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene denticleless E3 ubiquitin protein ligase homolog Neighboring gene RN7SK pseudogene 98 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:212299717-212300218 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2498 Neighboring gene ReSE screen-validated silencer GRCh37_chr1:212347425-212347676 Neighboring gene Sharpr-MPRA regulatory region 12199 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2500 Neighboring gene long intergenic non-protein coding RNA 2608 Neighboring gene ReSE screen-validated silencer GRCh37_chr1:212426200-212426446 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:212429519-212430020 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_3428 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_3455 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2502 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1793 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr1:212489099-212489852 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:212493252-212493848 Neighboring gene ribosomal protein L23a pseudogene 18 Neighboring gene MPRA-validated peak686 silencer Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:212540447-212541032 Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:212541033-212541616 Neighboring gene small nucleolar RNA, H/ACA box 16B Neighboring gene ReSE screen-validated silencer GRCh37_chr1:212558425-212558608 Neighboring gene proton activated chloride channel 1 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1794 Neighboring gene uncharacterized LOC105372906 Neighboring gene CRISPRi-validated cis-regulatory element chr1.11595 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1795

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Formation of stable attachments between kinetochores and microtubules depends on the B56-PP2A phosphatase. Foley EA, et al. Nat Cell Biol, 2011 Aug 28. PMID 21874008, Free PMC Article
  2. Negative regulation of CHK2 activity by protein phosphatase 2A is modulated by DNA damage. Freeman AK, et al. Cell Cycle, 2010 Feb 15. PMID 20160490, Free PMC Article
  3. MicroRNA-218 promotes cisplatin resistance in oral cancer via the PPP2R5A/Wnt signaling pathway. Zhuang Z, et al. Oncol Rep, 2017 Oct. PMID 28849187, Free PMC Article
  4. PPP2R5A: A multirole protein phosphatase subunit in regulating cancer development. Mao Z, et al. Cancer Lett, 2018 Feb 1. PMID 29175459
  5. Selective PP2A Enhancement through Biased Heterotrimer Stabilization. Leonard D, et al. Cell, 2020 Apr 30. PMID 32315618, Free PMC Article

See all (108) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A complex of BRCA2 and PP2A-B56 is required for DNA repair by homologous recombination.
    Title: A complex of BRCA2 and PP2A-B56 is required for DNA repair by homologous recombination.
  2. Selective PP2A Enhancement through Biased Heterotrimer Stabilization.
    Title: Selective PP2A Enhancement through Biased Heterotrimer Stabilization.
  3. Functional and Structural Insights into a Vif/PPP2R5 Complex Elucidated Using Patient HIV-1 Isolates and Computational Modeling.
    Title: Functional and Structural Insights into a Vif/PPP2R5 Complex Elucidated Using Patient HIV-1 Isolates and Computational Modeling.
  4. HIV-1 Vif Triggers Cell Cycle Arrest by Degrading Cellular PPP2R5 Phospho-regulators.
    Title: HIV-1 Vif Triggers Cell Cycle Arrest by Degrading Cellular PPP2R5 Phospho-regulators.
  5. PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore.
    Title: PP1 and PP2A Use Opposite Phospho-dependencies to Control Distinct Processes at the Kinetochore.
  6. Studies indicate protein phosphatase 2, regulatory subunit B', alpha isoform protein (PPP2R5A) plays an important role in many cellular activities.
    Title: PPP2R5A: A multirole protein phosphatase subunit in regulating cancer development.
  7. Overexpression of miR-218 led to inhibition of PPP2R5A expression, whereas knockdown of miR-218 increased PPP2R5A levels. Introduction of PPP2R5A abrogated miR218-mediated cell survival and drug resistance.
    Title: MicroRNA-218 promotes cisplatin resistance in oral cancer via the PPP2R5A/Wnt signaling pathway.
  8. A mitotic phosphorylation site on Cdc20, known to be a substrate of PP2A(B56), modulates APC/C(Cdc20) assembly.
    Title: The PP2A(B56) phosphatase promotes the association of Cdc20 with APC/C in mitosis.
  9. Results report that BubR1 and RepoMan bind directly to PP2A-B56 using an LSPIxE short linear motif (SLiM), where phosphorylation of the Ser residue enhances binding. RepoMan and BubR1 bind B56 using both hydrophobic and electrostatic interactions.
    Title: Expanding the PP2A Interactome by Defining a B56-Specific SLiM.
  10. cyclin E is specifically dephosphorylated at S384 by the PP2A-B56 phosphatase, thereby uncoupling cyclin E degradation from cyclin E-CDK2 activity
    Title: The PP2A-B56 Phosphatase Opposes Cyclin E Autocatalytic Degradation via Site-Specific Dephosphorylation.
Submit: New GeneRIF Correction See all GeneRIFs (22)

Phenotypes

Go to the top of the page Help

Review eQTL and phenotype association data in this region using PheGenI

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

Go to the top of the page Help

Protein interactions

Protein Gene Interaction Pubs
Tat tat HIV-1 Tat upregulates the total levels of PP2A protein and downregulates the inactive form of phosphorylated PP2A, which leads to inhibit hTERT activity directly or indirectly in CD4+ T cells PubMed
tat Protein phosphatase-2A (PP2A) and protein phosphatase-1 (PP1) regulate HIV-1 Tat-activated transcription through dephosphorylation of CDK9 PubMed
tat An increase in the amount of PP2A core enzyme with a concomitant decrease in the amount of PP2A holoenzyme inhibits HIV-1 Tat-stimulated transcription from the HIV-1 LTR promoter, indicating a role for PP2A in the modulation of HIV-1 gene expression PubMed
Vif vif HIV-1 Vif degrades PPP2R5A in CEM-T4 T cells PubMed
vif HIV-1 Vif binds PPP2R5A expression in CEM-T4 T cells PubMed
vif HIV-1 Vif downregulates PPP2R5A expression in a Cul5 dependent manner in CEM-T4 T cells PubMed
Vpr vpr Amino-acid peptide sequence (residues 77-92) of HIV-1 Vpr binds to PP2A1. The sequence from HIV-1 89.6 strain is a cell penetrating and death domain PubMed
vpr HIV-1 Vpr was found to upregulate PP2A in fission yeast PubMed
vpr HIV-1 Vpr forms a complex with HIV-1 nucleocapsid that directly activates PP2A PubMed
capsid gag The interaction of HIV-1 CA with human cellular protein phosphatase 2, regulatory subunit B', alpha (PPP2R5A) is identified by yeast two-hybrid screen PubMed
nucleocapsid gag HIV-1 Vpr forms a complex with HIV-1 nucleocapsid that directly activates PP2A PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Clone Names

  • MGC131915

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables kinase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
contributes_to kinase inhibitor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein phosphatase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to protein phosphatase activator activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein phosphatase regulator activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in negative regulation of protein localization to plasma membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in signal transduction IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in M band ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
located_in Z disc IDA
Inferred from Direct Assay
more info
 PubMed 
located_in centrosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chromosome, centromeric region IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein phosphatase type 2A complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of protein phosphatase type 2A complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein phosphatase type 2A complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform
Names
PR61alpha
protein phosphatase 2 regulatory subunit B', alpha

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001199756.2 → NP_001186685.1  serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 2

    See identical proteins and their annotated locations for NP_001186685.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) has an alternate 5' exon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1.
    Source sequence(s)
    AK302202, BP326450, L42373
    Consensus CDS
    CCDS55686.1
    UniProtKB/Swiss-Prot
    Q15172
    Related
    ENSP00000442866.1, ENST00000537030.3
    Conserved Domains (1) summary
    pfam01603
    Location:7 → 385
    B56; Protein phosphatase 2A regulatory B subunit (B56 family)

  2. NM_006243.4 → NP_006234.1  serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform isoform 1

    See identical proteins and their annotated locations for NP_006234.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AL451060, L42373
    Consensus CDS
    CCDS1503.1
    UniProtKB/Swiss-Prot
    B2R6D2, B7Z7L2, D3DT99, Q15172, Q2NL72, Q5VVB2, Q8TBI9
    Related
    ENSP00000261461.2, ENST00000261461.7
    Conserved Domains (1) summary
    pfam01603
    Location:64 → 442
    B56; Protein phosphatase 2A regulatory B subunit (B56 family)

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

    Range
    212285410..212361853
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060925.1 Alternate T2T-CHM13v2.0

    Range
    211531574..211606538
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q15172.1 GenPept UniProtKB/Swiss-Prot:Q15172

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous