sub subito [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: subprovided by FlyBase
Official Full Name: subitoprovided by FlyBase
Primary source: FLYBASE:FBgn0003545
Locus tag: Dmel_CG12298
See related: AllianceGenome:FB:FBgn0003545
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG12298; Dmel\CG12298; DmKlp54E; DmSub; Dub; KIF 20A; KIF20A/B; mei-1794; Mklp2; MKlp2; MKLP2; Sub; SUB; Subito
Summary: Enables gamma-tubulin complex binding activity and microtubule motor activity. Involved in several processes, including embryonic cleavage; meiotic nuclear division; and microtubule cytoskeleton organization. Located in meiotic spindle midzone and spindle pole. Part of microtubule associated complex. Colocalizes with spindle microtubule. Human ortholog(s) of this gene implicated in restrictive cardiomyopathy. Orthologous to several human genes including KIF20A (kinesin family member 20A). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: all
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Genomic context

Location:: 54E7-54E7; 2-84 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (17745500..17748078, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (13633005..13635583, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

N- and C-terminal domains of Subito, while not required for localization to the central spindle microtubules, have important roles regulating Subito.
Title: Kinesin 6 Regulation in Drosophila Female Meiosis by the Non-conserved N- and C- Terminal Domains.
Subito has undergone three single-gene transpositions and it is the nearest gene to the breakpoints of other single-gene transpositions and of two chromosomal inversions.
Title: Evidence for a gene involved in multiple and diverse rearrangements in the Drosophila genus.
Subito is required for establishing and/or maintaining the central spindle in Drosophila oocytes, and this substitutes for the role of centrosomes in organizing the bipolar spindle.
Title: The kinesinlike protein Subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes.
Encodes a kinesin-like protein required for meiotic spindle pole formation in oocytes.
Title: subito encodes a kinesin-like protein required for meiotic spindle pole formation in Drosophila melanogaster.
Subito localizes to the spindle midzone at anaphase and is required for the localization of Polo, Incenp and Aurora B.
Title: Kinesin 6 family member Subito participates in mitotic spindle assembly and interacts with mitotic regulators.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables gamma-tubulin complex binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in embryonic cleavage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in female meiosis chromosome segregation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in female meiosis chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gamma-tubulin complex localization	IDA Inferred from Direct Assay more info	PubMed
involved_in male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic spindle midzone assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in meiotic spindle organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in metaphase/anaphase transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle assembly involved in female meiosis	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of kinesin complex	IBA Inferred from Biological aspect of Ancestor more info
located_in meiotic spindle midzone	IDA Inferred from Direct Assay more info	PubMed
is_active_in microtubule	IBA Inferred from Biological aspect of Ancestor more info
part_of microtubule associated complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
colocalizes_with spindle microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in spindle midzone	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: subito

Names: CG12298-PA; CG12298-PB; Kinesin_A; double or nothing; double-or-nothing; sub-PA; sub-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

17745500..17748078 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_137416.3 → NP_611260.1 subito, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_611260.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9V877

UniProtKB/TrEMBL

A0A0B4LGW1, B6UYA4

Related

FBpp0086041

Conserved Domains (4) summary

cd00106
Location:89 → 477

KISc; Kinesin motor domain

pfam00225
Location:93 → 479

Kinesin; Kinesin motor domain

cd16269
Location:576 → 586

GBP_C; coiled coil [structural motif]

cl20817
Location:512 → 603

GBP_C; Guanylate-binding protein, C-terminal domain
NM_001299619.1 → NP_001286548.1 subito, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286548.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9V877

UniProtKB/TrEMBL

A0A0B4LGW1, B6UYA4

Conserved Domains (4) summary

cd00106
Location:89 → 477

KISc; Kinesin motor domain

pfam00225
Location:93 → 479

Kinesin; Kinesin motor domain

cd16269
Location:576 → 586

GBP_C; coiled coil [structural motif]

cl20817
Location:512 → 603

GBP_C; Guanylate-binding protein, C-terminal domain