U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

nwk nervous wreck [ Drosophila melanogaster (fruit fly) ]

Gene ID: 39052, updated on 2-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
nwkprovided by FlyBase
Official Full Name
nervous wreckprovided by FlyBase
Primary source
FLYBASE:FBgn0263456
Locus tag
Dmel_CG43479
See related
AllianceGenome:FB:FBgn0263456
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG43479; CG4641; CG4684; Dmel\CG43479; Nwk; NWK
Old locus tag
Dmel_CG4641; Dmel_CG4684
Summary
Enables phosphatidylinositol-3,4-bisphosphate binding activity and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in several processes, including negative regulation of synaptic assembly at neuromuscular junction; neuromuscular synaptic transmission; and regulation of actin filament organization. Located in neuromuscular junction and recycling endosome. Is expressed in several structures, including adult head; ganglia; larval nervous system; type I bouton; and ventral midline of embryo. Orthologous to several human genes including FCHSD2 (FCH and double SH3 domains 2). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs
all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See nwk in Genome Data Viewer
Location:
67A1-67A1; 3-28 cM
Exon count:
9
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (9137860..9145693)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (9130960..9138793)

Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene boule Neighboring gene Dihydropteridine reductase Neighboring gene Unconventional SNARE in the ER 1 Neighboring gene mir-4940 stem loop Neighboring gene Resistant to dieldrin Neighboring gene Solute carrier family 45 member 1

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Effects of PI(4,5)P(2) concentration on the F-BAR domain membrane binding as revealed by coarse-grained simulations. Stanishneva-Konovalova TB, et al. Proteins, 2019 Jul. PMID 30803020
  2. Assembly of actin filaments and microtubules in Nwk F-BAR-induced membrane deformations. Kelley CF, et al. Commun Integr Biol, 2015 Mar-Apr. PMID 26478768, Free PMC Article
  3. BAR-SH3 sorting nexins are conserved interacting proteins of Nervous wreck that organize synapses and promote neurotransmission. Ukken FP, et al. J Cell Sci, 2016 Jan 1. PMID 26567222, Free PMC Article
  4. Membrane Charge Directs the Outcome of F-BAR Domain Lipid Binding and Autoregulation. Kelley CF, et al. Cell Rep, 2015 Dec 22. PMID 26686642, Free PMC Article
  5. Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck. Stanishneva-Konovalova TB, et al. Proc Natl Acad Sci U S A, 2016 Sep 20. PMID 27601635, Free PMC Article

See all (70) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. This study examines the interactions of the F-BAR domain of nervous wreck (Nwk) with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 )-containing membranes using coarse-grained molecular dynamics.
    Title: Effects of PI(4,5)P(2) concentration on the F-BAR domain membrane binding as revealed by coarse-grained simulations.
  2. In Drosophila neurons, Nwk autoregulation restricts SH3a domain-dependent synaptopod formation, synaptic growth, and actin organization. Our results define structural rearrangements in Nwk that control F-BAR-membrane interactions as well as SH3 domain activities, and suggest that these two functions are tightly coordinated in vitro and in vivo.
    Title: Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck.
  3. SH3PX1 promotes neurotransmitter release presynaptically, at least in part through functional interactions with Nwk.
    Title: BAR-SH3 sorting nexins are conserved interacting proteins of Nervous wreck that organize synapses and promote neurotransmission.
  4. Nwk-induced scallops can be extended by cytoskeletal forces to produce protrusions at the plasma membrane.
    Title: Formation of membrane ridges and scallops by the F-BAR protein Nervous Wreck.
  5. The results define a presynaptic trafficking pathway mediated by SNX16, NWK, and the ESCRT complex that functions to control synaptic growth signaling at the interface between endosomal compartments.
    Title: A presynaptic endosomal trafficking pathway controls synaptic growth signaling.
  6. Synaptic growth activated by growth factor signaling is controlled at an endosomal compartment via coordinated Nwk and Cdc42-dependent actin assembly.
    Title: Nervous wreck and Cdc42 cooperate to regulate endocytic actin assembly during synaptic growth.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables phosphatidylinositol-3,4-bisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphatidylinositol-4,5-bisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables phospholipid binding ISM
Inferred from Sequence Model
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in membrane organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of synaptic assembly at neuromuscular junction IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of synaptic assembly at neuromuscular junction IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neuromuscular synaptic transmission IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in neuromuscular synaptic transmission IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of Arp2/3 complex-mediated actin nucleation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in receptor recycling IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of actin filament polymerization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of actin filament polymerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of synaptic assembly at neuromuscular junction IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in neuromuscular junction IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in neuromuscular junction IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in recycling endosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in recycling endosome IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
nervous wreck
Names
CG43479-PB
CG43479-PC
CG43479-PD
CG43479-PF
CG43479-PG
CG43479-PH
CG43479-PI
CG43479-PJ
CG43479-PK
CG43479-PL
nwk-PB
nwk-PC
nwk-PD
nwk-PF
nwk-PG
nwk-PH
nwk-PI
nwk-PJ
nwk-PK
nwk-PL

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_037436.4 Reference assembly

    Range
    9137860..9145693
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001259762.2 → NP_001246691.1  nervous wreck, isoform D [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001246691.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q9VSU8
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:663 → 718
    SH3; Src Homology 3 domain superfamily

  2. NM_001300067.1 → NP_001286996.1  nervous wreck, isoform K [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001286996.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    X2JC77
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:663 → 718
    SH3; Src Homology 3 domain superfamily

  3. NM_001259763.2 → NP_001246692.1  nervous wreck, isoform F [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001246692.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9NF56
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:663 → 718
    SH3; Src Homology 3 domain superfamily

  4. NM_140033.3 → NP_648290.3  nervous wreck, isoform G [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_648290.3

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q9VSU8
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:663 → 718
    SH3; Src Homology 3 domain superfamily

  5. NM_001300065.1 → NP_001286994.1  nervous wreck, isoform I [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001286994.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    M9NE66, M9NF56, M9NFQ1, Q9VSU8, X2J8V0, X2JAU8, X2JC77, X2JCQ1, X2JGF9
    Conserved Domains (4) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    PRK12323
    Location:725 → 956
    PRK12323; DNA polymerase III subunits gamma and tau; Provisional
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:663 → 718
    SH3; Src Homology 3 domain superfamily

  6. NM_001300066.1 → NP_001286995.1  nervous wreck, isoform J [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001286995.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    X2JGF9
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:663 → 718
    SH3; Src Homology 3 domain superfamily

  7. NM_001300068.1 → NP_001286997.1  nervous wreck, isoform L [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001286997.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    X2JCQ1
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:640 → 695
    SH3; Src Homology 3 domain superfamily

  8. NM_001259760.2 → NP_001246689.1  nervous wreck, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001246689.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9NE66
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:640 → 695
    SH3; Src Homology 3 domain superfamily

  9. NM_001300064.1 → NP_001286993.1  nervous wreck, isoform H [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001286993.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    X2J8V0
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:640 → 695
    SH3; Src Homology 3 domain superfamily

  10. NM_001259761.2 → NP_001246690.1  nervous wreck, isoform C [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001246690.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9NFQ1
    Conserved Domains (3) summary
    cd11761
    Location:545 → 600
    SH3_FCHSD_1; First Src Homology 3 domain of FCH and double SH3 domains proteins
    cl12013
    Location:16 → 281
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17036
    Location:663 → 718
    SH3; Src Homology 3 domain superfamily
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9VSU9 Q9VSU9
X2JAU8.1 X2JAU8

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Molecular Biology Databases
Research Materials
Tools