Vps35 Vacuolar protein sorting 35 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Vps35provided by FlyBase
Official Full Name: Vacuolar protein sorting 35provided by FlyBase
Primary source: FLYBASE:FBgn0034708
Locus tag: Dmel_CG5625
See related: AllianceGenome:FB:FBgn0034708
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG5625; Dmel\CG5625; DmVps35; dvps35; dVPS35; Dvps35; DVps35; vps35; VPS35
Summary: Enables Notch binding activity and small GTPase binding activity. Involved in several processes, including neuron differentiation; positive regulation of cell communication; and positive regulation of endosome to plasma membrane protein transport. Located in endosome; presynaptic active zone; and subsynaptic reticulum. Is expressed in several structures, including anterior endoderm; anterior endoderm anlage; circulatory system; embryonic/larval midgut; and extended germ band embryo. Used to study Parkinson's disease and brain cancer. Human ortholog(s) of this gene implicated in Parkinson's disease and Parkinson's disease 17. Orthologous to human VPS35 (VPS35 retromer complex component). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 58C7-58C7; 2-98 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (22185972..22189886)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (18073477..18077391)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The in vivo effects of P316S, D620N and L774M of VPS35 using Drosophila as a model, were investigated.
Title: In vivo evidence of pathogenicity of VPS35 mutations in the Drosophila.
The findings of this study indicate that the VPS35 may play a crucial role in alpha-synucle in degradation by modulating the maturation of cathepsin D and might thereby contribute to the pathogenesis of the disease.
Title: VPS35 dysfunction impairs lysosomal degradation of α-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease.
overexpression of Vps35 ameliorates the pathogenic mutant LRRK2 eye phenotype in a Drosophila model of Parkinson's disease
Title: Vacuolar protein sorting 35 (Vps35) rescues locomotor deficits and shortened lifespan in Drosophila expressing a Parkinson's disease mutant of Leucine-Rich Repeat Kinase 2 (LRRK2).
Vps35 negatively regulates actin polymerisation, and genetic interactions suggest that some of the endocytic and signalling defects of vps35 mutants are due to this function
Title: Drosophila Vps35 function is necessary for normal endocytic trafficking and actin cytoskeleton organisation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endosome to plasma membrane protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of Wnt protein secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein stability	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synaptic vesicle endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to rotenone	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde transport, endosome to Golgi	IBA Inferred from Biological aspect of Ancestor more info
involved_in retrograde transport, endosome to Golgi	IC Inferred by Curator more info	PubMed
involved_in retrograde transport, endosome to Golgi	IEA Inferred from Electronic Annotation more info
involved_in retrograde transport, endosome to Golgi	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde transport, endosome to Golgi	ISS Inferred from Sequence or Structural Similarity more info
involved_in synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in dopaminergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in dopaminergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	ISS Inferred from Sequence or Structural Similarity more info
is_active_in late endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in late endosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic endocytic zone	IDA Inferred from Direct Assay more info	PubMed
part_of retromer complex	IBA Inferred from Biological aspect of Ancestor more info
part_of retromer complex	NAS Non-traceable Author Statement more info	PubMed
part_of retromer, cargo-selective complex	IEA Inferred from Electronic Annotation more info
part_of retromer, cargo-selective complex	ISS Inferred from Sequence or Structural Similarity more info
located_in subsynaptic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: vacuolar protein sorting 35

Names: CG5625-PA; CG5625-PB; Vps35-PA; Vps35-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

22185972..22189886

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_166518.3 → NP_726175.3 vacuolar protein sorting 35, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726175.3

Status: REVIEWED

UniProtKB/TrEMBL

Q6AWP5, Q9W277

Conserved Domains (1) summary

pfam03635
Location:32 → 777

Vps35; Vacuolar protein sorting-associated protein 35
NM_137807.4 → NP_611651.4 vacuolar protein sorting 35, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_611651.4

Status: REVIEWED

UniProtKB/TrEMBL

Q6AWP5, Q7KVL7

Conserved Domains (1) summary

pfam03635
Location:13 → 758

Vps35; Vacuolar protein sorting-associated protein 35