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PKN3 protein kinase N3 [ Homo sapiens (human) ]

Gene ID: 29941, updated on 14-Nov-2024

Summary

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Official Symbol
PKN3provided by HGNC
Official Full Name
protein kinase N3provided by HGNC
Primary source
HGNC:HGNC:17999
See related
Ensembl:ENSG00000160447 MIM:610714; AllianceGenome:HGNC:17999
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
UTDP4-1
Summary
Predicted to enable protein serine/threonine kinase activity. Involved in epithelial cell migration. Predicted to be located in Golgi apparatus; cytosol; and nucleus. [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in fat (RPKM 3.9), lung (RPKM 3.1) and 25 other tissues See more
Orthologs
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Genomic context

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See PKN3 in Genome Data Viewer
Location:
9q34.11
Exon count:
22
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 9 NC_000009.12 (128702503..128720916)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 9 NC_060933.1 (140907265..140925683)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 9 NC_000009.11 (131464782..131483195)

Chromosome 9 - NC_000009.12Genomic Context describing neighboring genes Neighboring gene dynein 2 intermediate chain 2 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:131430084-131430584 Neighboring gene high mobility group AT-hook 1 pseudogene 4 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:131445235-131445871 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:131445872-131446509 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29088 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29089 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29090 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:131449798-131450674 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20347 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20348 Neighboring gene SET nuclear proto-oncogene Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:131465059-131465598 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:131475223-131475724 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr9:131475725-131476224 Neighboring gene RNA, 7SL, cytoplasmic 560, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20350 Neighboring gene ZDHHC12 divergent transcript Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:131491015-131491846 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr9:131491847-131492678 Neighboring gene zinc finger DHHC-type palmitoyltransferase 12 Neighboring gene H3K27ac hESC enhancer GRCh37_chr9:131494472-131494972 Neighboring gene H3K27ac hESC enhancer GRCh37_chr9:131494973-131495473 Neighboring gene zyg-11 related cell cycle regulator Neighboring gene small nucleolar RNA U13

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Identification and characterization of PKNbeta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKNalpha. Oishi K, et al. Biochem Biophys Res Commun, 1999 Aug 11. PMID 10441506
  2. Depletion of protein kinase N3 (PKN3) impairs actin and adherens junctions dynamics and attenuates endothelial cell activation. Möpert K, et al. Eur J Cell Biol, 2012 Sep. PMID 22609186
  3. PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase. Leenders F, et al. EMBO J, 2004 Aug 18. PMID 15282551, Free PMC Article
  4. PKN3 is the major regulator of angiogenesis and tumor metastasis in mice. Mukai H, et al. Sci Rep, 2016 Jan 8. PMID 26742562, Free PMC Article
  5. A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18. Dibus M, et al. Int J Mol Sci, 2020 Oct 20. PMID 33092266, Free PMC Article

See all (48) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18.
    Title: A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18.
  2. a novel interaction of p130Cas with Ser/Thr kinase PKN3, is reported.
    Title: The interaction of p130Cas with PKN3 promotes malignant growth.
  3. Steady-state kinetic analysis revealed that PKN1-3 follows a sequential ordered Bi-Bi kinetic mechanism, where peptide substrate binding is preceded by ATP binding. This kinetic mechanism was confirmed by additional kinetic studies for product inhibition and affinity of small molecule inhibitors.
    Title: Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors.
  4. PKN3 is the major regulator of angiogenesis in humans and mice and tumor metastasis in mice.
    Title: PKN3 is the major regulator of angiogenesis and tumor metastasis in mice.
  5. PKN3 can be considered a novel protein implicated in remodeling the actin-adherens junction, possibly by linking ICAM-1-signaling with actin/AJ dynamics.
    Title: Depletion of protein kinase N3 (PKN3) impairs actin and adherens junctions dynamics and attenuates endothelial cell activation.
  6. PKN isoforms are not simply redundant in supporting migration, but appear to be linked through isoform specific regulatory domain properties to selective upstream signals. It
    Title: Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration.
  7. PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of phosphoinositide 3-kinase
    Title: PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genetic variants associated with disordered eating.
EBI GWAS Catalog
Nine loci for ocular axial length identified through genome-wide association studies, including shared loci with refractive error.
EBI GWAS Catalog
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables calcium,diacylglycerol-dependent serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase activity TAS
Traceable Author Statement
more info
 PubMed 
enables protein serine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables small GTPase binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in epithelial cell migration IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in intracellular signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein phosphorylation TAS
Traceable Author Statement
more info
 PubMed 
involved_in signal transduction TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus TAS
Traceable Author Statement
more info
 PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in perinuclear region of cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
serine/threonine-protein kinase N3
Names
protein kinase PKN-beta
protein-kinase C-related kinase 3
NP_001304855.1
NP_037487.2
XP_005252003.1
XP_006717143.1
XP_016870138.1
XP_016870139.1
XP_047279234.1
XP_047279235.1
XP_054218798.1
XP_054218799.1
XP_054218800.1
XP_054218801.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001317926.2 → NP_001304855.1  serine/threonine-protein kinase N3 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the penultimate exon, compared to variant 1, resulting in a novel 3' coding region and shorter 3' UTR. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AL441992
    Conserved Domains (6) summary
    cd11622
    Location:13 → 78
    HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N
    cd11632
    Location:97 → 170
    HR1_PKN3_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N3
    smart00220
    Location:559 → 811
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cl00087
    Location:170 → 230
    HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
    cl14603
    Location:328 → 410
    C2; C2 domain
    cl21453
    Location:559 → 815
    PKc_like; Protein Kinases, catalytic domain

  2. NM_013355.5 → NP_037487.2  serine/threonine-protein kinase N3 isoform 1

    See identical proteins and their annotated locations for NP_037487.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AL441992
    Consensus CDS
    CCDS6908.1
    UniProtKB/Swiss-Prot
    Q6P5Z2, Q9UM03
    Related
    ENSP00000291906.4, ENST00000291906.5
    Conserved Domains (6) summary
    PTZ00263
    Location:555 → 879
    PTZ00263; protein kinase A catalytic subunit; Provisional
    cd11622
    Location:13 → 78
    HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N
    cd11632
    Location:97 → 170
    HR1_PKN3_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N3
    cd05589
    Location:559 → 884
    STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N
    cl00087
    Location:170 → 230
    HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
    cl14603
    Location:328 → 410
    C2; C2 domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000009.12 Reference GRCh38.p14 Primary Assembly

    Range
    128702503..128720916
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017014650.2 → XP_016870139.1  serine/threonine-protein kinase N3 isoform X4

  2. XM_047423278.1 → XP_047279234.1  serine/threonine-protein kinase N3 isoform X4

  3. XM_005251946.4 → XP_005252003.1  serine/threonine-protein kinase N3 isoform X2

    Conserved Domains (6) summary
    PTZ00263
    Location:552 → 876
    PTZ00263; protein kinase A catalytic subunit; Provisional
    cd11622
    Location:10 → 75
    HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N
    cd11632
    Location:94 → 167
    HR1_PKN3_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N3
    cl00087
    Location:167 → 227
    HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
    cl14603
    Location:325 → 407
    C2; C2 domain
    cl21453
    Location:556 → 881
    PKc_like; Protein Kinases, catalytic domain

  4. XM_006717080.4 → XP_006717143.1  serine/threonine-protein kinase N3 isoform X1

    Conserved Domains (6) summary
    cd11622
    Location:20 → 85
    HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N
    cd11632
    Location:104 → 177
    HR1_PKN3_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N3
    smart00220
    Location:566 → 825
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd05589
    Location:566 → 891
    STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N
    cl00087
    Location:177 → 237
    HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
    cl14603
    Location:335 → 417
    C2; C2 domain

  5. XM_017014649.3 → XP_016870138.1  serine/threonine-protein kinase N3 isoform X3

  6. XM_047423279.1 → XP_047279235.1  serine/threonine-protein kinase N3 isoform X4

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060933.1 Alternate T2T-CHM13v2.0

    Range
    140907265..140925683
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054362826.1 → XP_054218801.1  serine/threonine-protein kinase N3 isoform X4

  2. XM_054362825.1 → XP_054218800.1  serine/threonine-protein kinase N3 isoform X3

  3. XM_054362823.1 → XP_054218798.1  serine/threonine-protein kinase N3 isoform X1

  4. XM_054362824.1 → XP_054218799.1  serine/threonine-protein kinase N3 isoform X2

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q6P5Z2.1 GenPept UniProtKB/Swiss-Prot:Q6P5Z2

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