U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

ANPEP alanyl aminopeptidase, membrane [ Homo sapiens (human) ]

Gene ID: 290, updated on 3-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
ANPEPprovided by HGNC
Official Full Name
alanyl aminopeptidase, membraneprovided by HGNC
Primary source
HGNC:HGNC:500
See related
Ensembl:ENSG00000166825 MIM:151530; AllianceGenome:HGNC:500
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
APN; AP-M; AP-N; CD13; LAP1; P150; PEPN; hAPN; GP150
Summary
Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma. [provided by RefSeq, Apr 2020]
Annotation information
Note: This gene has been reviewed for its involvement in coronavirus biology, and is relevant for disease process.
Expression
Biased expression in duodenum (RPKM 1193.1), small intestine (RPKM 1007.5) and 5 other tissues See more
Orthologs
mouse all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See ANPEP in Genome Data Viewer
Location:
15q26.1
Exon count:
21
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 15 NC_000015.10 (89784895..89814852, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 15 NC_060939.1 (87540783..87570713, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 15 NC_000015.9 (90328126..90358084, complement)

Chromosome 15 - NC_000015.10Genomic Context describing neighboring genes Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6805 Neighboring gene mitochondrial ribosomal protein L15 pseudogene 1 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:90310867-90311584 Neighboring gene uncharacterized LOC124903550 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6806 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 10055 Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr15:90326293-90326955 Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr15:90326956-90327617 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:90327618-90328280 Neighboring gene mesoderm posterior bHLH transcription factor 2 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:90335518-90336018 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr15:90347169-90348368 Neighboring gene ANPEP proximal promoter Neighboring gene FOXA motif-containing MPRA enhancer 170 Neighboring gene ANPEP distal promoter Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:90365008-90365508 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:90365509-90366009 Neighboring gene uncharacterized LOC124903551 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:90367927-90368600 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 10056 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:90370583-90371474 Neighboring gene ARPIN-AP3S2 readthrough Neighboring gene uncharacterized LOC124903552 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr15:90380077-90381276 Neighboring gene adaptor related protein complex 3 subunit sigma 2

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

Go to the top of the pageHelp

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. ANPEP/CD13 Expression as a Marker of Lymphovascular Invasion and Survival in Esophageal Adenocarcinoma.
    Title: ANPEP/CD13 Expression as a Marker of Lymphovascular Invasion and Survival in Esophageal Adenocarcinoma.
  2. CD13 expression affects glioma patient survival and influences key functions of human glioblastoma cell lines in vitro.
    Title: CD13 expression affects glioma patient survival and influences key functions of human glioblastoma cell lines in vitro.
  3. CD13 facilitates immune cell migration and aggravates acute injury but promotes chronic post-stroke recovery.
    Title: CD13 facilitates immune cell migration and aggravates acute injury but promotes chronic post-stroke recovery.
  4. Aminopeptidase N/CD13 Crosslinking Promotes the Activation and Membrane Expression of Integrin CD11b/CD18.
    Title: Aminopeptidase N/CD13 Crosslinking Promotes the Activation and Membrane Expression of Integrin CD11b/CD18.
  5. Altered serum levels of platelet-derived growth factor receptor beta and cluster of differentiation 13 suggest a role for pericytes in West syndrome.
    Title: Altered serum levels of platelet-derived growth factor receptor β and cluster of differentiation 13 suggest a role for pericytes in West syndrome.
  6. Reduced expression of alanyl aminopeptidase is a robust biomarker of non-familial adenomatous polyposis and non-hereditary nonpolyposis colorectal cancer syndrome early-onset colorectal cancer.
    Title: Reduced expression of alanyl aminopeptidase is a robust biomarker of non-familial adenomatous polyposis and non-hereditary nonpolyposis colorectal cancer syndrome early-onset colorectal cancer.
  7. Soluble ANPEP Released From Human Astrocytes as a Positive Regulator of Microglial Activation and Neuroinflammation: Brain Renin-Angiotensin System in Astrocyte-Microglia Crosstalk.
    Title: Soluble ANPEP Released From Human Astrocytes as a Positive Regulator of Microglial Activation and Neuroinflammation: Brain Renin-Angiotensin System in Astrocyte-Microglia Crosstalk.
  8. Abnormal CD13/HLA-DR Expression Pattern on Myeloblasts Predicts Development of Myeloid Neoplasia in Patients With Clonal Cytopenia of Undetermined Significance.
    Title: Abnormal CD13/HLA-DR Expression Pattern on Myeloblasts Predicts Development of Myeloid Neoplasia in Patients With Clonal Cytopenia of Undetermined Significance.
  9. [The Relationship between the Expressions of CD33 and CD13 and the Prognosis of Patients with Multiple Myeloma].
    Title: [The Relationship between the Expressions of CD33 and CD13 and the Prognosis of Patients with Multiple Myeloma].
  10. CD13 is a critical regulator of cell-cell fusion in osteoclastogenesis.
    Title: CD13 is a critical regulator of cell-cell fusion in osteoclastogenesis.
Submit: New GeneRIF Correction See all GeneRIFs (115)

Phenotypes

Go to the top of the page Help

Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
An atlas of genetic influences on human blood metabolites.
EBI GWAS Catalog

Variation

Go to the top of the page Help

See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables aminopeptidase activity TAS
Traceable Author Statement
more info
 PubMed 
enables metalloaminopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metallopeptidase activity TAS
Traceable Author Statement
more info
 PubMed 
enables peptide binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables signaling receptor activity TAS
Traceable Author Statement
more info
 PubMed 
enables virus receptor activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in angiogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in cell differentiation IEA
Inferred from Electronic Annotation
more info
  
involved_in peptide catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in proteolysis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in symbiont entry into host cell IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum-Golgi intermediate compartment IDA
Inferred from Direct Assay
more info
 PubMed 
located_in external side of plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in extracellular exosome HDA PubMed 
located_in extracellular exosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extracellular space HDA PubMed 
is_active_in extracellular space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in extracellular space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in lysosomal membrane HDA PubMed 
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane TAS
Traceable Author Statement
more info
  
located_in secretory granule membrane TAS
Traceable Author Statement
more info
  

General protein information

Go to the top of the page Help
Preferred Names
aminopeptidase N
Names
alanyl (membrane) aminopeptidase
aminopeptidase M
membrane alanyl aminopeptidase
microsomal aminopeptidase
myeloid plasma membrane glycoprotein CD13
NP_001141.2
NP_001368852.1
NP_001368853.1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001150.3 → NP_001141.2  aminopeptidase N precursor

    See identical proteins and their annotated locations for NP_001141.2

    Status: REVIEWED

    Source sequence(s)
    AB209918, AC018988, AC079075, BC058928, BQ879097
    Consensus CDS
    CCDS10356.1
    UniProtKB/Swiss-Prot
    P15144, Q16728, Q6GT90, Q8IUK3, Q8IVH3, Q9UCE0
    UniProtKB/TrEMBL
    Q59E93
    Related
    ENSP00000300060.6, ENST00000300060.7
    Conserved Domains (2) summary
    cd09601
    Location:84 → 545
    M1_APN-Q_like; Peptidase M1 aminopeptidase N catalytic domain family which includes aminopeptidase N (APN), aminopeptidase Q (APQ), tricorn interacting factor F3, and endoplasmic reticulum aminopeptidase 1 (ERAP1)
    pfam11838
    Location:619 → 946
    ERAP1_C; ERAP1-like C-terminal domain

  2. NM_001381923.1 → NP_001368852.1  aminopeptidase N

    Status: REVIEWED

    Source sequence(s)
    AC018988, AC079075
    Consensus CDS
    CCDS10356.1
    UniProtKB/Swiss-Prot
    P15144, Q16728, Q6GT90, Q8IUK3, Q8IVH3, Q9UCE0
    UniProtKB/TrEMBL
    Q59E93
    Related
    ENSP00000453413.2, ENST00000560137.2
    Conserved Domains (2) summary
    cd09601
    Location:84 → 545
    M1_APN-Q_like; Peptidase M1 aminopeptidase N catalytic domain family which includes aminopeptidase N (APN), aminopeptidase Q (APQ), tricorn interacting factor F3, and endoplasmic reticulum aminopeptidase 1 (ERAP1)
    pfam11838
    Location:619 → 946
    ERAP1_C; ERAP1-like C-terminal domain

  3. NM_001381924.1 → NP_001368853.1  aminopeptidase N

    Status: REVIEWED

    Source sequence(s)
    AC018988, AC079075
    Consensus CDS
    CCDS10356.1
    UniProtKB/Swiss-Prot
    P15144, Q16728, Q6GT90, Q8IUK3, Q8IVH3, Q9UCE0
    UniProtKB/TrEMBL
    Q59E93
    Conserved Domains (2) summary
    cd09601
    Location:84 → 545
    M1_APN-Q_like; Peptidase M1 aminopeptidase N catalytic domain family which includes aminopeptidase N (APN), aminopeptidase Q (APQ), tricorn interacting factor F3, and endoplasmic reticulum aminopeptidase 1 (ERAP1)
    pfam11838
    Location:619 → 946
    ERAP1_C; ERAP1-like C-terminal domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000015.10 Reference GRCh38.p14 Primary Assembly

    Range
    89784895..89814852 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060939.1 Alternate T2T-CHM13v2.0

    Range
    87540783..87570713 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P15144.4 GenPept UniProtKB/Swiss-Prot:P15144

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous