Snap25 synaptosome associated protein 25 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Snap25provided by RGD
Official Full Name: synaptosome associated protein 25provided by RGD
Primary source: RGD:3728
See related: EnsemblRapid:ENSRNOG00000006037 AllianceGenome:RGD:3728
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: SNAP-25B; SNAP-25a
Summary: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein. It is essential for regulated exocytosis in neuronal cells. Alternative transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2012]
Expression: Restricted expression toward (RPKM 1950.9) See more
Orthologs: human mouse all
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Genomic context

Location:: 3q36

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (144494579..144576449)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (124041898..124123761)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (129697408..129788417)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Carbohydrate response element-binding protein (ChREBP) mediates decreased SNAP25 expression in islets from diabetic Goto-Kakizaki (GK) rats.
Title: Carbohydrate response element-binding protein (ChREBP) mediates decreased SNAP25 expression in islets from diabetic Goto-Kakizaki (GK) rats.
BDNF/TrkB signalling, in cooperation with muscarinic signalling, retrogradely regulates PKA pathway to phosphorylate SNAP-25 and Synapsin-1 at the neuromuscular junction.
Title: BDNF/TrkB signalling, in cooperation with muscarinic signalling, retrogradely regulates PKA pathway to phosphorylate SNAP-25 and Synapsin-1 at the neuromuscular junction.
GLP-1 signaling-regulated SNAP25 is involved in improved insulin secretion in diabetic GK rats after Roux-en-Y gastric bypass surgery.
Title: GLP-1 signaling-regulated SNAP25 is involved in improved insulin secretion in diabetic GK rats after Roux-en-Y gastric bypass surgery.
Snap25 attenuates neuronal injury via reducing ferroptosis in acute ischemic stroke.
Title: Snap25 attenuates neuronal injury via reducing ferroptosis in acute ischemic stroke.
SNAP23 decreases insulin secretion by competitively inhibiting the interaction between SNAP25 and STX1A.
Title: SNAP23 decreases insulin secretion by competitively inhibiting the interaction between SNAP25 and STX1A.
Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis.
Title: Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis.
Munc18-dependent and -independent clustering of syntaxin in the plasma membrane of cultured endocrine cells.
Title: Munc18-dependent and -independent clustering of syntaxin in the plasma membrane of cultured endocrine cells.
The mesoscale organization of syntaxin 1A and SNAP25 is determined by SNARE-SNARE interactions.
Title: The mesoscale organization of syntaxin 1A and SNAP25 is determined by SNARE-SNARE interactions.
Changes in the Expression of SNAP-25 Protein in the Brain of Juvenile Rats in Two Models of Autism.
Title: Changes in the Expression of SNAP-25 Protein in the Brain of Juvenile Rats in Two Models of Autism.
Synaptotagmin-1-, Munc18-1-, and Munc13-1-dependent liposome fusion with a few neuronal SNAREs.
Title: Synaptotagmin-1-, Munc18-1-, and Munc13-1-dependent liposome fusion with a few neuronal SNAREs.

Submit: New GeneRIF Correction See all GeneRIFs (94)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BE107867 (e-PCR)
- UniSTS:247097

D3Wox20 (e-PCR)
- UniSTS:118108

D2Nds33 (e-PCR), detects polymorphism
- UniSTS:118108

D25 (e-PCR)
- UniSTS:129162

D3M2Mit28 (e-PCR)
- UniSTS:129162

D2Mit28.1 (e-PCR), detects polymorphism
- UniSTS:129163

D2Mit28.3 (e-PCR), detects polymorphism
- UniSTS:129164

D3Kyo3 (e-PCR)
- UniSTS:240323

Snap25 (e-PCR), detects polymorphism
- UniSTS:532437

MARC_11717-11718:997472340:1 (e-PCR)
- UniSTS:267259

MARC_11717-11718:1029180244:1 (e-PCR)
- UniSTS:267259

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables SNAP receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables SNAP receptor activity	IEA Inferred from Electronic Annotation more info
enables SNAP receptor activity	ISO Inferred from Sequence Orthology more info
enables SNAP receptor activity	TAS Traceable Author Statement more info	PubMed
enables SNARE binding	IDA Inferred from Direct Assay more info	PubMed
enables SNARE binding	IPI Inferred from Physical Interaction more info	PubMed
enables SNARE binding	ISO Inferred from Sequence Orthology more info
enables calcium-dependent protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables myosin binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin-1 binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin-1 binding	IDA Inferred from Direct Assay more info	PubMed
enables syntaxin-1 binding	IEA Inferred from Electronic Annotation more info
enables syntaxin-1 binding	IPI Inferred from Physical Interaction more info	PubMed
enables syntaxin-1 binding	ISO Inferred from Sequence Orthology more info
enables transmembrane transporter binding	IPI Inferred from Physical Interaction more info	PubMed
enables voltage-gated potassium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables voltage-gated potassium channel activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in SNARE complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in associative learning	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within associative learning	ISO Inferred from Sequence Orthology more info
involved_in axonogenesis	IEP Inferred from Expression Pattern more info	PubMed
involved_in calcium ion-regulated exocytosis of neurotransmitter	IDA Inferred from Direct Assay more info	PubMed
involved_in endosomal transport	IDA Inferred from Direct Assay more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IEA Inferred from Electronic Annotation more info
involved_in exocytosis	TAS Traceable Author Statement more info	PubMed
involved_in locomotory behavior	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within locomotory behavior	ISO Inferred from Sequence Orthology more info
involved_in long-term memory	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-term synaptic potentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within long-term synaptic potentiation	ISO Inferred from Sequence Orthology more info
involved_in membrane fusion	IEA Inferred from Electronic Annotation more info
involved_in neuron differentiation	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within neurotransmitter secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of hormone secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of insulin secretion	IEP Inferred from Expression Pattern more info	PubMed
involved_in potassium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in presynaptic dense core vesicle exocytosis	IEA Inferred from Electronic Annotation more info
involved_in presynaptic dense core vesicle exocytosis	ISO Inferred from Sequence Orthology more info
involved_in regulation of establishment of protein localization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of establishment of protein localization	ISO Inferred from Sequence Orthology more info
involved_in regulation of neuron projection development	IEA Inferred from Electronic Annotation more info
involved_in regulation of neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in regulation of synapse assembly	IEP Inferred from Expression Pattern more info	PubMed
involved_in synaptic vesicle exocytosis	EXP Inferred from Experiment more info	PubMed
involved_in synaptic vesicle exocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in synaptic vesicle exocytosis	IEP Inferred from Expression Pattern more info	PubMed
involved_in synaptic vesicle exocytosis	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle fusion to presynaptic active zone membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic vesicle priming	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of BLOC-1 complex	IEA Inferred from Electronic Annotation more info
part_of BLOC-1 complex	ISO Inferred from Sequence Orthology more info
part_of BLOC-1 complex	ISS Inferred from Sequence or Structural Similarity more info
part_of SNARE complex	IDA Inferred from Direct Assay more info	PubMed
part_of SNARE complex	IEA Inferred from Electronic Annotation more info
part_of SNARE complex	ISO Inferred from Sequence Orthology more info
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IEA Inferred from Electronic Annotation more info
located_in axon	ISO Inferred from Sequence Orthology more info
located_in axonal growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in cell cortex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in filopodium	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IEA Inferred from Electronic Annotation more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in photoreceptor inner segment	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in presynaptic membrane	IEA Inferred from Electronic Annotation more info
located_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
located_in ribbon synapse	IEA Inferred from Electronic Annotation more info
located_in ribbon synapse	ISO Inferred from Sequence Orthology more info
located_in somatodendritic compartment	IEA Inferred from Electronic Annotation more info
located_in somatodendritic compartment	ISO Inferred from Sequence Orthology more info
located_in synapse	IEA Inferred from Electronic Annotation more info
located_in synapse	ISO Inferred from Sequence Orthology more info
located_in synaptic vesicle	IEA Inferred from Electronic Annotation more info
located_in synaptic vesicle	ISO Inferred from Sequence Orthology more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	IDA Inferred from Direct Assay more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex	IBA Inferred from Biological aspect of Ancestor more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex	IDA Inferred from Direct Assay more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex	IEA Inferred from Electronic Annotation more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex	ISO Inferred from Sequence Orthology more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex	IDA Inferred from Direct Assay more info	PubMed
located_in terminal bouton	HDA more info	PubMed
located_in trans-Golgi network	IEA Inferred from Electronic Annotation more info
located_in trans-Golgi network	ISO Inferred from Sequence Orthology more info
located_in vesicle	ISO Inferred from Sequence Orthology more info
part_of voltage-gated potassium channel complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: synaptosomal-associated protein 25

Names: SNAP-25; SUP; super protein; synaptosomal-associated 25 kDa protein; synaptosomal-associated protein, 25 kDa

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001270575.1 → NP_001257504.1 synaptosomal-associated protein 25 isoform a

See identical proteins and their annotated locations for NP_001257504.1

Status: REVIEWED

Description

Transcript Variant: This variant (1, also known as a) encodes isoform a.

Source sequence(s)

BC087699, DY315301, FQ214162

UniProtKB/TrEMBL

F8WG75

Related

ENSRNOP00000007998.7, ENSRNOT00000007998.9

Conserved Domains (3) summary

cd15885
Location:143 → 201

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

cd15894
Location:10 → 82

SNARE_SNAP25N; N-terminal SNARE motif of SNAP25

pfam00835
Location:91 → 141

SNAP-25; SNAP-25 family
NM_001270576.1 → NP_001257505.1 synaptosomal-associated protein 25 isoform a

See identical proteins and their annotated locations for NP_001257505.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Both variants 1 and 2 encode the same isoform a.

Source sequence(s)

BC087699, FM033263

UniProtKB/TrEMBL

F8WG75

Related

ENSRNOP00000008734.7, ENSRNOT00000008734.9

Conserved Domains (3) summary

cd15885
Location:143 → 201

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

cd15894
Location:10 → 82

SNARE_SNAP25N; N-terminal SNARE motif of SNAP25

pfam00835
Location:91 → 141

SNAP-25; SNAP-25 family
NM_030991.3 → NP_112253.1 synaptosomal-associated protein 25 isoform b

See identical proteins and their annotated locations for NP_112253.1

Status: REVIEWED

Description

Transcript Variant: This variant (3, also known as b) has an alternate in-frame exon in the coding region, compared to variant 1. The resulting isoform (b) is of the same length, but has a distinct internal segment, compared to isoform a.

Source sequence(s)

BC087699, FQ214162

UniProtKB/Swiss-Prot

P60881

UniProtKB/TrEMBL

A6HQK1, A6HQK2

Related

ENSRNOP00000072612.2, ENSRNOT00000080581.3

Conserved Domains (3) summary

cd15885
Location:143 → 201

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

cd15894
Location:10 → 82

SNARE_SNAP25N; N-terminal SNARE motif of SNAP25

pfam00835
Location:91 → 141

SNAP-25; SNAP-25 family

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086021.1 Reference GRCr8

Range

144494579..144576449

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063283127.1 → XP_063139197.1 synaptosomal-associated protein 25 isoform X2
XM_039104314.2 → XP_038960242.1 synaptosomal-associated protein 25 isoform X1

UniProtKB/Swiss-Prot

P60881

UniProtKB/TrEMBL

A6HQK1, A6HQK2

Conserved Domains (3) summary

cd15885
Location:143 → 201

SNARE_SNAP25C; C-terminal SNARE motif of SNAP25

cd15894
Location:10 → 82

SNARE_SNAP25N; N-terminal SNARE motif of SNAP25

pfam00835
Location:91 → 141

SNAP-25; SNAP-25 family
XM_063283128.1 → XP_063139198.1 synaptosomal-associated protein 25 isoform X3