TRIM65 tripartite motif containing 65 [Homo sapiens (human)] - Gene

Summary

Official Symbol: TRIM65provided by HGNC
Official Full Name: tripartite motif containing 65provided by HGNC
Primary source: HGNC:HGNC:27316
See related: Ensembl:ENSG00000141569 MIM:619408; AllianceGenome:HGNC:27316
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: 4732463G12Rik
Summary: Enables ubiquitin protein ligase activity. Involved in several processes, including positive regulation of autophagy; positive regulation of interferon-beta production; and protein polyubiquitination. Located in cytosol and nucleoplasm. Is active in cytoplasm. [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in spleen (RPKM 5.1), lung (RPKM 4.3) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 17q25.1

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	17	NC_000017.11 (75879537..75896951, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	17	NC_060941.1 (76771866..76790102, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	17	NC_000017.10 (73885044..73893032, complement)

Chromosome 17 - NC_000017.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

TRIM65 Suppresses oxLDL-induced Endothelial Inflammation by Interaction with VCAM-1 in Atherogenesis.
Title: TRIM65 Suppresses oxLDL-induced Endothelial Inflammation by Interaction with VCAM-1 in Atherogenesis.
TRIM65/NF2/YAP1 Signaling Coordinately Orchestrates Metabolic and Immune Advantages in Hepatocellular Carcinoma.
Title: TRIM65/NF2/YAP1 Signaling Coordinately Orchestrates Metabolic and Immune Advantages in Hepatocellular Carcinoma.
Tripartite Motif-Containing Protein 65 (TRIM65) Inhibits Hepatitis B Virus Transcription.
Title: Tripartite Motif-Containing Protein 65 (TRIM65) Inhibits Hepatitis B Virus Transcription.
TRIM65 promotes vascular smooth muscle cell phenotypic transformation by activating PI3K/Akt/mTOR signaling during atherogenesis.
Title: TRIM65 promotes vascular smooth muscle cell phenotypic transformation by activating PI3K/Akt/mTOR signaling during atherogenesis.
TRIM65 knockout inhibits the development of HCC by polarization tumor-associated macrophages towards M1 phenotype via JAK1/STAT1 signaling pathway.
Title: TRIM65 knockout inhibits the development of HCC by polarization tumor-associated macrophages towards M1 phenotype via JAK1/STAT1 signaling pathway.
TRIM65 Promotes Malignant Cell Behaviors in Triple-Negative Breast Cancer by Impairing the Stability of LATS1 Protein.
Title: TRIM65 Promotes Malignant Cell Behaviors in Triple-Negative Breast Cancer by Impairing the Stability of LATS1 Protein.
TRIM65 determines the fate of a novel subtype of pituitary neuroendocrine tumors via ubiquitination and degradation of TPIT.
Title: TRIM65 determines the fate of a novel subtype of pituitary neuroendocrine tumors via ubiquitination and degradation of TPIT.
Knockdown of TRIM65 suppressed the proliferation and invasiveness of gastric cancer cells by restricting the ubiquitin degradation of PPM1A.
Title: Knockdown of TRIM65 suppressed the proliferation and invasiveness of gastric cancer cells by restricting the ubiquitin degradation of PPM1A.
TRIM65 in White Matter Lesions, Innate Immunity, and Tumor.
Title: TRIM65 in White Matter Lesions, Innate Immunity, and Tumor.
LncRNA LINC00963 promotes colorectal cancer cell proliferation and metastasis by regulating miR1281 and TRIM65.
Title: LncRNA LINC00963 promotes colorectal cancer cell proliferation and metastasis by regulating miR‑1281 and TRIM65.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association studies of cerebral white matter lesion burden: the CHARGE consortium. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

G65616 (e-PCR)
- UniSTS:225552

SHGC-132666 (e-PCR)
- UniSTS:185381

RH79813 (e-PCR)
- UniSTS:92198

SHGC-155088 (e-PCR)
- UniSTS:183084

D8S2279 (e-PCR)
- UniSTS:473907

D15S1477 (e-PCR)
- UniSTS:474482

D17S1475E (e-PCR)
- UniSTS:151682

D11S3114 (e-PCR)
- UniSTS:152207

SHGC-37061 (e-PCR)
- UniSTS:5788

RH78964 (e-PCR)
- UniSTS:28121

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in antiviral innate immune response	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of inflammatory response	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of autophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interferon-alpha production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of interferon-beta production	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein oligomerization	IEA Inferred from Electronic Annotation more info
involved_in protein K48-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein K63-linked ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in type I interferon-mediated signaling pathway	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase TRIM65

Names: tripartite motif-containing protein 65

NP_001243053.1

EC 2.3.2.27

NP_775818.2

EC 2.3.2.27

XP_006721823.1

EC 2.3.2.27

XP_006721824.1

EC 2.3.2.27

XP_011522801.1

EC 2.3.2.27

XP_011522802.1

EC 2.3.2.27

XP_011522803.1

EC 2.3.2.27

XP_011522804.1

EC 2.3.2.27

XP_011522805.1

EC 2.3.2.27

XP_016879833.1

EC 2.3.2.27

XP_047291536.1

EC 2.3.2.27

XP_047291537.1

EC 2.3.2.27

XP_047291539.1

EC 2.3.2.27

XP_047291540.1

EC 2.3.2.27

XP_047291541.1

EC 2.3.2.27

XP_054171375.1

EC 2.3.2.27

XP_054171376.1

EC 2.3.2.27

XP_054171377.1

EC 2.3.2.27

XP_054171378.1

EC 2.3.2.27

XP_054171379.1

EC 2.3.2.27

XP_054171380.1

EC 2.3.2.27

XP_054171381.1

EC 2.3.2.27

XP_054171382.1

EC 2.3.2.27

XP_054171383.1

EC 2.3.2.27

XP_054171384.1

EC 2.3.2.27

XP_054171385.1

EC 2.3.2.27

XP_054171386.1

EC 2.3.2.27

XP_054171387.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001256124.2 → NP_001243053.1 E3 ubiquitin-protein ligase TRIM65 isoform 2

See identical proteins and their annotated locations for NP_001243053.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.

Source sequence(s)

AC087289, BC021259, BG701649, BG753016, DR002345

UniProtKB/Swiss-Prot

Q6PJ69

Conserved Domains (4) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

pfam15898
Location:123 → 173

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cd12896
Location:307 → 479

SPRY_PRY_TRIM65; PRY/SPRY domain in tripartite motif-containing domain 65 (TRIM65)
NM_173547.4 → NP_775818.2 E3 ubiquitin-protein ligase TRIM65 isoform 1

See identical proteins and their annotated locations for NP_775818.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC087289, AI242308, BC021259

Consensus CDS

CCDS11732.1

UniProtKB/Swiss-Prot

Q4G0F0, Q6DKJ6, Q6PJ69, Q9BRP6

Related

ENSP00000269383.3, ENST00000269383.8

Conserved Domains (4) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

pfam15898
Location:123 → 173

PRKG1_interact; cGMP-dependent protein kinase interacting domain

cd12896
Location:322 → 501

SPRY_PRY_TRIM65; PRY/SPRY domain in tripartite motif-containing domain 65 (TRIM65)

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000017.11 Reference GRCh38.p14 Primary Assembly

Range

75879537..75896951 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047435584.1 → XP_047291540.1 E3 ubiquitin-protein ligase TRIM65 isoform X10
XM_047435583.1 → XP_047291539.1 E3 ubiquitin-protein ligase TRIM65 isoform X7
XM_011524501.3 → XP_011522803.1 E3 ubiquitin-protein ligase TRIM65 isoform X8

Conserved Domains (3) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cl02614
Location:320 → 362

SPRY; SPRY domain
XM_011524499.3 → XP_011522801.1 E3 ubiquitin-protein ligase TRIM65 isoform X5

Conserved Domains (3) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cl02614
Location:335 → 384

SPRY; SPRY domain
XM_011524502.3 → XP_011522804.1 E3 ubiquitin-protein ligase TRIM65 isoform X9

Conserved Domains (3) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cl02614
Location:320 → 353

SPRY; SPRY domain
XM_011524500.3 → XP_011522802.1 E3 ubiquitin-protein ligase TRIM65 isoform X6

Conserved Domains (3) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cl02614
Location:335 → 375

SPRY; SPRY domain
XM_047435585.1 → XP_047291541.1 E3 ubiquitin-protein ligase TRIM65 isoform X11
XM_017024344.2 → XP_016879833.1 E3 ubiquitin-protein ligase TRIM65 isoform X13

UniProtKB/TrEMBL

H0YGS7

Related

ENSP00000444607.1, ENST00000540128.1

Conserved Domains (2) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
XM_047435581.1 → XP_047291537.1 E3 ubiquitin-protein ligase TRIM65 isoform X2
XM_011524503.3 → XP_011522805.1 E3 ubiquitin-protein ligase TRIM65 isoform X12

UniProtKB/TrEMBL

H0YGS7

Conserved Domains (2) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
XM_006721761.4 → XP_006721824.1 E3 ubiquitin-protein ligase TRIM65 isoform X4

Conserved Domains (3) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cd12896
Location:320 → 492

SPRY_PRY_TRIM65; PRY/SPRY domain in tripartite motif-containing domain 65 (TRIM65)
XM_006721760.4 → XP_006721823.1 E3 ubiquitin-protein ligase TRIM65 isoform X3

Conserved Domains (3) summary

cd00021
Location:94 → 130

BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.

cd00162
Location:11 → 54

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

cd12896
Location:335 → 514

SPRY_PRY_TRIM65; PRY/SPRY domain in tripartite motif-containing domain 65 (TRIM65)
XM_047435580.1 → XP_047291536.1 E3 ubiquitin-protein ligase TRIM65 isoform X1