DDX5 DEAD-box helicase 5 [Homo sapiens (human)] - Gene

Summary

Official Symbol: DDX5provided by HGNC
Official Full Name: DEAD-box helicase 5provided by HGNC
Primary source: HGNC:HGNC:2746
See related: Ensembl:ENSG00000108654 MIM:180630; AllianceGenome:HGNC:2746
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: p68; HLR1; G17P1; HUMP68
Summary: This gene encodes a member of the DEAD box family of RNA helicases that are involved in a variety of cellular processes as a result of its role as an adaptor molecule, promoting interactions with a large number of other factors. This protein is involved in pathways that include the alteration of RNA structures, plays a role as a coregulator of transcription, a regulator of splicing, and in the processing of small noncoding RNAs. Members of this family contain nine conserved motifs, including the conserved Asp-Glu-Ala-Asp (DEAD) motif, important to ATP binding and hydrolysis as well as RNA binding and unwinding activities. Dysregulation of this gene may play a role in cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2017]
Expression: Ubiquitous expression in bone marrow (RPKM 190.1), lymph node (RPKM 152.4) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 17q23.3

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	17	NC_000017.11 (64498254..64506866, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	17	NC_060941.1 (65368010..65376622, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	17	NC_000017.10 (62494372..62502984, complement)

Chromosome 17 - NC_000017.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

AURKAIP1 actuates tumor progression through stabilizing DDX5 in triple negative breast cancer.
Title: AURKAIP1 actuates tumor progression through stabilizing DDX5 in triple negative breast cancer.
DDX5 (p68) orchestrates beta-catenin, RelA and SP1 mediated MGMT gene expression in human colon cancer cells: Implication in TMZ chemoresistance.
Title: DDX5 (p68) orchestrates β-catenin, RelA and SP1 mediated MGMT gene expression in human colon cancer cells: Implication in TMZ chemoresistance.
Role of the DEAD-box RNA helicase DDX5 (p68) in cancer DNA repair, immune suppression, cancer metabolic control, virus infection promotion, and human microbiome (microbiota) negative influence.
Title: Role of the DEAD-box RNA helicase DDX5 (p68) in cancer DNA repair, immune suppression, cancer metabolic control, virus infection promotion, and human microbiome (microbiota) negative influence.
Hypoxia-mediated regulation of DDX5 through decreased chromatin accessibility and post-translational targeting restricts R-loop accumulation.
Title: Hypoxia-mediated regulation of DDX5 through decreased chromatin accessibility and post-translational targeting restricts R-loop accumulation.
The DEAD-box RNA helicase DDX5 (p68) and beta-catenin: The crucial regulators of FOXM1 gene expression in arbitrating colorectal cancer.
Title: The DEAD-box RNA helicase DDX5 (p68) and β-catenin: The crucial regulators of FOXM1 gene expression in arbitrating colorectal cancer.
Splicing factor SRSF3 promotes the progression of cervical cancer through regulating DDX5.
Title: Splicing factor SRSF3 promotes the progression of cervical cancer through regulating DDX5.
Cleavage of AUF1 by Coxsackievirus B Affects DDX5 Regulatory on Viral Replication through iTRAQ Proteomics Analysis.
Title: Cleavage of AUF1 by Coxsackievirus B Affects DDX5 Regulatory on Viral Replication through iTRAQ Proteomics Analysis.
The RNA helicase DDX5 cooperates with EHMT2 to sustain alveolar rhabdomyosarcoma growth.
Title: The RNA helicase DDX5 cooperates with EHMT2 to sustain alveolar rhabdomyosarcoma growth.
P68 RNA Helicase (DDX5) Required for the Formation of Various Specific and Mature miRNA Active RISC Complexes.
Title: P68 RNA Helicase (DDX5) Required for the Formation of Various Specific and Mature miRNA Active RISC Complexes.
Knockdown of KRAB domain-associated protein 1 suppresses the proliferation, migration and invasion of thyroid cancer cells by regulating P68/DEAD box protein 5.
Title: Knockdown of KRAB domain-associated protein 1 suppresses the proliferation, migration and invasion of thyroid cancer cells by regulating P68/DEAD box protein 5.

Submit: New GeneRIF Correction See all GeneRIFs (109)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
siRNA knockdown of DDX5 decreases intra- and extra-cellular HIV CA(p24) from HeLa cells transfected with env-deleted HIV-1 plasmid, a vesicular stomatitis virus glycoprotein plasmid and specific siRNA. Resulting HIV-1 demonstrates decreased infectivity.	PubMed
siRNA knockdown of DDX5 decreases HIV RNA and consequently virus production as measured by CA (p24) and infectivity by 2-3 times compared with siControl-treated cells (HeLa-M and TZM-bl)	PubMed
Knockdown of DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5) by siRNA inhibits HIV-1 replication in primary CD4+ T cells	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Gag-Pol	gag-pol	Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Nef	nef	Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Pr55(Gag)	gag	HIV-1 Gag interacts with RPS6 as demonstrated by proximity dependent biotinylation proteomics	PubMed
	gag	Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Rev	rev	DDX5 enhances the Rev-dependent HIV-1 RNA export and the interaction between DDX5 and Rev is largely dependent on RNA	PubMed
	rev	DDX5 interacts with HIV-1 Rev and enhances the Rev function. DDX5 co-localizes with Rev in both nucleolus and nucleus	PubMed
	rev	DDX5 interacts with DDX1, DDX3, or DDX21 and synergistically enhances the Rev function	PubMed
	rev	HIV-1 Rev interacting protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with DDX5 is increased by RRE	PubMed
Vpr	vpr	A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5) expression by HIV-1 Vpr in Vpr transduced macrophages	PubMed
capsid	gag	siRNA knockdown of DDX5 decreases intra- and extra-cellular HIV CA(p24) from HeLa cells transfected with env-deleted HIV-1 plasmid, a vesicular stomatitis virus glycoprotein plasmid and specific siRNA. Resulting HIV-1 demonstrates decreased infectivity.	PubMed
	gag	Knockdown of DDX5 by siRNA inhibits HIV-1 replication with downregulated levels of HIV-1 CA expression in CD4+ T cells	PubMed
matrix	gag	HIV-1 MA upregulates DDX5 gene expression in HepG2 cells	PubMed
retropepsin	gag-pol	Positional proteomics analysis identifies the cleavage of human DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5) at amino acid residues 96-97 by the HIV-1 protease	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC102575P1 (e-PCR)
- UniSTS:270088

WI-22548 (e-PCR)
- UniSTS:77080

MARC_2724-2725:991933343:1 (e-PCR)
- UniSTS:230942

RH11006 (e-PCR)
- UniSTS:71760

RH104075 (e-PCR)
- UniSTS:98400

D17S1990 (e-PCR)
- UniSTS:2178

Ddx5 (e-PCR), detects polymorphism
- UniSTS:506841

RH103168 (e-PCR)
- UniSTS:97501

DDX5 (e-PCR)
- UniSTS:266725

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables MH2 domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables R-SMAD binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA binding	HDA more info	PubMed
enables RNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA helicase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA helicase activity	NAS Non-traceable Author Statement more info	PubMed
enables SMAD binding	IPI Inferred from Physical Interaction more info	PubMed
enables calcium-dependent protein binding	IEA Inferred from Electronic Annotation more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables nuclear androgen receptor binding	IDA Inferred from Direct Assay more info	PubMed
enables pre-mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables primary miRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables promoter-specific chromatin binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ribonucleoprotein complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables ribonucleoprotein complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in BMP signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in alternative mRNA splicing, via spliceosome	IBA Inferred from Biological aspect of Ancestor more info
involved_in alternative mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in androgen receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epithelial to mesenchymal transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular estrogen receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intrinsic apoptotic signaling pathway by p53 class mediator	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA splicing, via spliceosome	IC Inferred by Curator more info	PubMed
involved_in mRNA transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in myoblast differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of DNA damage response, signal transduction by p53 class mediator	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in primary miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of alternative mRNA splicing, via spliceosome	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of alternative mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of androgen receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in regulation of miRNA transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of osteoblast differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of skeletal muscle cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of viral genome replication	IEA Inferred from Electronic Annotation more info
involved_in rhythmic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of catalytic step 2 spliceosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular exosome	HDA more info	PubMed
located_in membrane	HDA more info	PubMed
located_in nuclear speck	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
part_of ribonucleoprotein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: probable ATP-dependent RNA helicase DDX5

Names: ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); RNA helicase p68

NP_001307524.1

EC 3.6.4.13

NP_001307525.1

EC 3.6.4.13

NP_001307526.1

EC 3.6.4.13

NP_004387.1

EC 3.6.4.13

XP_047291469.1

EC 3.6.4.13

XP_054171273.1

EC 3.6.4.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001320595.2 → NP_001307524.1 probable ATP-dependent RNA helicase DDX5 isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 through 3 encode the same isoform.

Source sequence(s)

AC009994, AC234063

Consensus CDS

CCDS11659.1

UniProtKB/Swiss-Prot

B4DLW8, B5BU21, D3DU32, E7ETL9, O75681, P17844, Q53Y61

UniProtKB/TrEMBL

B5BUE6

Related

ENSP00000463168.2, ENST00000585111.2

Conserved Domains (2) summary

PTZ00110
Location:6 → 507

PTZ00110; helicase; Provisional

pfam08061
Location:498 → 532

P68HR; P68HR (NUC004) repeat
NM_001320596.3 → NP_001307525.1 probable ATP-dependent RNA helicase DDX5 isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 through 3 encode the same isoform (a).

Source sequence(s)

AC234063, AI653967, BC016027, BP230373, DA918007

Consensus CDS

CCDS11659.1

UniProtKB/Swiss-Prot

B4DLW8, B5BU21, D3DU32, E7ETL9, O75681, P17844, Q53Y61

UniProtKB/TrEMBL

B5BUE6

Related

ENSP00000403085.3, ENST00000450599.7

Conserved Domains (2) summary

PTZ00110
Location:6 → 507

PTZ00110; helicase; Provisional

pfam08061
Location:498 → 532

P68HR; P68HR (NUC004) repeat
NM_001320597.2 → NP_001307526.1 probable ATP-dependent RNA helicase DDX5 isoform b

Status: REVIEWED

Description

Transcript Variant: This variant (4) has a different 5' structure, resulting in a novel 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (b) has a distinct N-terminus, and is the same length as isoform a.

Source sequence(s)

AK310318, BM695261, BU160611, CN280686, DB231101, N67237

Consensus CDS

CCDS82190.1

UniProtKB/TrEMBL

B5BUE6, J3KTA4

Related

ENSP00000462885.1, ENST00000578804.5

Conserved Domains (2) summary

pfam08061
Location:551 → 583

P68HR; P68HR (NUC004) repeat

cl26939
Location:14 → 507

DEXDc; DEAD-like helicases superfamily
NM_004396.5 → NP_004387.1 probable ATP-dependent RNA helicase DDX5 isoform a

See identical proteins and their annotated locations for NP_004387.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 through 3 encode the same isoform (a).

Source sequence(s)

BC016027, BM695261, BU160611, CN280686, DB133401, N67237

Consensus CDS

CCDS11659.1

UniProtKB/Swiss-Prot

B4DLW8, B5BU21, D3DU32, E7ETL9, O75681, P17844, Q53Y61

UniProtKB/TrEMBL

B5BUE6

Related

ENSP00000225792.5, ENST00000225792.10

Conserved Domains (2) summary

PTZ00110
Location:6 → 507

PTZ00110; helicase; Provisional

pfam08061
Location:498 → 532

P68HR; P68HR (NUC004) repeat