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Cenpf centromere protein F [ Mus musculus (house mouse) ]

Gene ID: 108000, updated on 2-Nov-2024

Summary

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Official Symbol
Cenpfprovided by MGI
Official Full Name
centromere protein Fprovided by MGI
Primary source
MGI:MGI:1313302
See related
Ensembl:ENSMUSG00000026605 AllianceGenome:MGI:1313302
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
CENF; Lek1; PRO1779; mitosin; 6530404A22Rik
Summary
Predicted to enable DNA-binding transcription factor binding activity; dynein complex binding activity; and protein homodimerization activity. Acts upstream of or within regulation of striated muscle tissue development. Located in cytoskeleton and pronucleus. Part of ciliary transition fiber. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and limb. Used to study dilated cardiomyopathy. Human ortholog(s) of this gene implicated in Stromme syndrome. Orthologous to human CENPF (centromere protein F). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Biased expression in CNS E11.5 (RPKM 9.3), liver E14 (RPKM 4.4) and 10 other tissues See more
Orthologs
human all
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Genomic context

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See Cenpf in Genome Data Viewer
Location:
1 H6; 1 95.03 cM
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 1 NC_000067.7 (189372803..189420302, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 1 NC_000067.6 (189640606..189688107, complement)

Chromosome 1 - NC_000067.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 46177 Neighboring gene STARR-seq mESC enhancer starr_03397 Neighboring gene STARR-seq mESC enhancer starr_03398 Neighboring gene STARR-positive B cell enhancer ABC_E3351 Neighboring gene predicted gene, 30088 Neighboring gene STARR-positive B cell enhancer ABC_E802 Neighboring gene STARR-positive B cell enhancer mm9_chr1:191524821-191525121 Neighboring gene STARR-seq mESC enhancer starr_03400 Neighboring gene STARR-seq mESC enhancer starr_03401 Neighboring gene STARR-seq mESC enhancer starr_03402 Neighboring gene protein tyrosine phosphatase, non-receptor type 14 Neighboring gene predicted gene, 51739 Neighboring gene CapStarr-seq enhancer MGSCv37_chr1:191627745-191627946 Neighboring gene STARR-seq mESC enhancer starr_03406 Neighboring gene predicted gene, 30280

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Apoptosis-induced translocation of centromere protein F in its corresponding autoantibody production in hepatocellular carcinoma. Li X, et al. Oncoimmunology, 2021. PMID 34676150, Free PMC Article
  2. Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities. Pfaltzgraff ER, et al. Mol Biol Cell, 2016 Jul 1. PMID 27146114, Free PMC Article
  3. LEK1 protein expression in normal and dysregulated cardiomyocyte mitosis. Dees E, et al. Anat Rec A Discov Mol Cell Evol Biol, 2005 Sep. PMID 16047383
  4. Conserved C-terminal domains of mCenp-F (LEK1) regulate subcellular localization and mitotic checkpoint delay. Evans HJ, et al. Exp Cell Res, 2007 Jul 1. PMID 17498689, Free PMC Article
  5. Loss of CENPF leads to developmental failure in mouse embryos. Zhou CJ, et al. Cell Cycle, 2019 Oct. PMID 31478449, Free PMC Article

See all (64) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.
    Title: CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.
  2. Apoptosis-induced translocation of centromere protein F in its corresponding autoantibody production in hepatocellular carcinoma.
    Title: Apoptosis-induced translocation of centromere protein F in its corresponding autoantibody production in hepatocellular carcinoma.
  3. Loss of CENPF leads to developmental failure in mouse embryos.
    Title: Loss of CENPF leads to developmental failure in mouse embryos.
  4. Loss of CENP-F Function Induces Kidney Enlargement.
    Title: Malformations in the Murine Kidney Caused by Loss of CENP-F Function.
  5. Results found that loss of function mutation in CENPF results in dilated cardiomyopathy with significant disruption in the microtubule network of cardiac myocytes in mouse.
    Title: Loss of CENP-F Results in Dilated Cardiomyopathy with Severe Disruption of Cardiac Myocyte Architecture.
  6. Due to the multifunctionality of CENP-F, the cellular phenotypes observed upon its depletion are difficult to interpret and there is a need to genetically separate its different functions by preventing binding to selected partners. Here we engineer a CENP-F point-mutant that is deficient in Miro1/2 binding and thus is unable to localize to mitochondria, but retains other localizations.
    Title: Miro-dependent mitochondrial pool of CENP-F and its farnesylated C-terminal domain are dispensable for normal development in mice.
  7. Our data show that mutation of the CENP-F gene leads to an unexpected hyperstabilization of the MT network, with a unique loss of dynamic instability. With disruption of MT dynamics, CENP-F-/- cells exhibit dramatic loss of directionally persistent migration, defects in focal adhesion disassembly and lamellipodial formation/retraction, change in cilia frequency, and loss of regulation of cell shape.
    Title: Loss of CENP-F results in distinct microtubule-related defects without chromosomal abnormalities.
  8. FOXM1 and CENPF are master regulators of prostate cancer malignancy, and can serve as drug response markers for antineoplastic drugs efficiency.
    Title: Predicting Drug Response in Human Prostate Cancer from Preclinical Analysis of In Vivo Mouse Models.
  9. Loss of CENP-F function in developing myocytes leads to decreased cell division, blunting of trabeculation and an initially smaller, thin-walled heart.
    Title: Cardiac-specific deletion of the microtubule-binding protein CENP-F causes dilated cardiomyopathy.
  10. These data demonstrate that CMF1 regulates myocyte differentiation by interaction with Rb family members to induce expression of myogenic regulatory factors.
    Title: CMF1-Rb interaction promotes myogenesis in avian skeletal myoblasts.
Submit: New GeneRIF Correction See all GeneRIFs (18)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA-binding transcription factor binding ISO
Inferred from Sequence Orthology
more info
  
enables dynein complex binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dynein complex binding ISO
Inferred from Sequence Orthology
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in chromosome segregation ISO
Inferred from Sequence Orthology
more info
  
involved_in metaphase chromosome alignment IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in metaphase chromosome alignment ISO
Inferred from Sequence Orthology
more info
  
involved_in mitotic cell cycle IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitotic cell cycle ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in protein transport ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of G2/M transition of mitotic cell cycle IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of G2/M transition of mitotic cell cycle ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within regulation of striated muscle tissue development IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in axoneme IDA
Inferred from Direct Assay
more info
 PubMed 
located_in centrosome IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in chromosome, centromeric region IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in chromosome, centromeric region ISO
Inferred from Sequence Orthology
more info
 PubMed 
located_in ciliary basal body IDA
Inferred from Direct Assay
more info
 PubMed 
part_of ciliary transition fiber IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
NOT located_in kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
located_in kinetochore ISO
Inferred from Sequence Orthology
more info
  
located_in midbody ISO
Inferred from Sequence Orthology
more info
  
located_in nuclear envelope ISO
Inferred from Sequence Orthology
more info
  
located_in nuclear matrix ISO
Inferred from Sequence Orthology
more info
  
located_in nuclear membrane ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
part_of outer kinetochore ISO
Inferred from Sequence Orthology
more info
  
located_in pronucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle ISO
Inferred from Sequence Orthology
more info
  
is_active_in spindle pole IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in spindle pole ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
centromere protein F
Names
centromere autoantigen F
leucine, glutamic acid, lysine family 1 protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001081363.2 → NP_001074832.2  centromere protein F

    Status: VALIDATED

    Source sequence(s)
    AC140679
    Consensus CDS
    CCDS35820.2
    UniProtKB/TrEMBL
    E9Q3P4, Q155P7
    Related
    ENSMUSP00000129738.2, ENSMUST00000171929.8
    Conserved Domains (5) summary
    COG0419
    Location:915 → 1363
    SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]
    TIGR02168
    Location:290 → 1122
    SMC_prok_B; chromosome segregation protein SMC, common bacterial type
    pfam10473
    Location:2313 → 2449
    CENP-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1
    pfam10481
    Location:1 → 300
    CENP-F_N; Cenp-F N-terminal domain
    pfam10490
    Location:2850 → 2896
    CENP-F_C_Rb_bdg; Rb-binding domain of kinetochore protein Cenp-F/LEK1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000067.7 Reference GRCm39 C57BL/6J

    Range
    189372803..189420302 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006497119.4 → XP_006497182.1  centromere protein F isoform X1

    UniProtKB/TrEMBL
    Q155P7
    Conserved Domains (6) summary
    COG0419
    Location:843 → 1291
    SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]
    COG1196
    Location:1865 → 2702
    Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]
    TIGR02168
    Location:218 → 1050
    SMC_prok_B; chromosome segregation protein SMC, common bacterial type
    pfam10473
    Location:2241 → 2377
    CENP-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1
    pfam10481
    Location:1 → 228
    CENP-F_N; Cenp-F N-terminal domain
    pfam10490
    Location:2778 → 2824
    CENP-F_C_Rb_bdg; Rb-binding domain of kinetochore protein Cenp-F/LEK1
Nucleotide Protein Strain
Heading Accession and Version

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