GEO DataSets

(Submitter supplied) The translation inhibitor Linezolid is an important antibiotic of last resort against multiresistant gram-positive pathogens including MRSA. Linezolid is reported to specifically inhibit extracellular virulence factors, but the molecular cause is unknown. To elucidate the physiological response of S. aureus to Linezolid in general and the possible inhibition of virulence factors specifically we performed a holistic study. more...

Organism:

Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

15 Samples

Download data: TXT

(Submitter supplied) Mutants from SG511 and NCTC8325 (wild type isolates) resistant to daptomycin were obtained following serial passage experiments in vitro. Transcriptomic experiments showed alteration in the expression of genes involved in various pathways including general metabolism, cell wall regulon and lipid metabolism.

Organism:

Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus COL

Type:

Expression profiling by array

Platform:

GPL7137

4 Samples

Download data: TXT

(Submitter supplied) With a copy number of about 25,000 molecules per cell, Asp23 is one of the most abundant proteins in Staphylococcus aureus. Asp23 has been characterized as a protein with an apparent molecular mass of 23 kDa that, following an alkaline shock, accumulates in the soluble protein fraction. Moreover, it was shown that transcription of the asp23 gene is exclusively regulated by the alternative sigma factor B. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

12 Samples

Download data: TXT

(Submitter supplied) A mutant of SG511 (wild type isolate), resistant to the presence of chitosan (polycationic molecule) was obtained following serial passage experiments resistance to chitosan exposition. Transcriptomic experiments showed alteration in the expression of genes involved in the cell wall regulon resulting in the modification of susceptibility phenotype against cell wall active antibiotics

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MW2

Type:

Expression profiling by array

Platform:

GPL7137

3 Samples

Download data: TXT

(Submitter supplied) The phenotype “intermediate vancomycin resistance” in Staphylococcus aureus (CLSI: MIC = 4-8 mg/L) has been assigned to changes that lead to alterations in cell wall synthesis and morphology. Most vancomycin intermediately resistant S. aureus (VISA) strains are characterised by an increased cell wall thickness as a consequence of an activated cell wall biosynthesis and decreased autolysis. The purpose of this study was to analyse the genetic basis of the vancomycin resistance mechanism of the clinical VISA isolate SA137/93A and its spontaneous mutant strain SA137/93G. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus N315

Type:

Expression profiling by array

Platforms:

GPL5421 GPL5431

8 Samples

Download data: GPR

(Submitter supplied) Methicillin resistant Staphylococcus aureus (MRSA) infection is becoming refractory to existing antibiotic therapy owing to the inherent ability of S. aureus to develop rapid resistance and is considered a major threat to public health. We found that a natural isolate of Bacillus pumilus from the Columbia River Estuary produces a strong anti-MRSA compound, amicoumacin A. As amicoumacin A has been reported to exhibit anti-microbial, anti-inflammatory, and anti-ulcer activities, we sought to uncover its mechanism of action. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

9 Samples

Download data: TXT

(Submitter supplied) Objectives: Development of daptomycin resistance (DAPR) in Staphylococcus aureus is associated with clinical treatment failures. Mechanism(s) of such resistance has not been clearly defined. Methods: We studied an isogenic daptomycin-susceptible (DAPS) and daptomycin-resistant (DAPR) S. aureus strain pair (616; 701) from a patient with relapsing endocarditis during daptomycin treatment, using comparative transcriptomic and proteomic techniques. more...

Organism:

Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

6 Samples

Download data: TXT

(Submitter supplied) The alternative sigma factor σB of Staphylococcus aureus is involved in the coordination of the general stress response, expression of virulence determinants and modulation of antibiotic resistance levels. It controls a large regulon, either directly by recognizing conserved σB promoter sequences, or indirectly via σB-dependent elements. The σB-controlled yabJ-spoVG operon encodes two such putative downstream elements. more...

Organism:

Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL7137

9 Samples

Download data: TXT

(Submitter supplied) The opportunistic pathogen, Staphylococcus aureus, encounters a wide variety of fluid shear levels within the human host, which may play a key role in dictating whether this organism adopts a commensal interaction with the host or transitions to cause disease. Using rotating-wall vessel bioreactors to create a physiologically-relevant, low fluid shear environment, S. aureus was evaluated for cellular responses that could impact its colonization and virulence. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus N315

Type:

Expression profiling by array

Platform:

GPL1339

4 Samples

Download data: CEL, CHP

(Submitter supplied) Screening of various bisquaternary bisnaphthalimides against a variety of human pathogens revealed one compound, designated MT02, with strong inhibitory effects against gram-positive bacteria. The minimal inhibitory concentrations ranged from 0.31 µg/ml against community-acquired methicillin resistant Staphylococcus aureus (MRSA) strain USA300 to 20 µg/ml against Streptococcus pneumonia. DNA-microarray studies generated a transcriptional signature characterized by a strong increase of genes involved in DNA-metabolism, DNA-replication, SOS-response and transport of positively charged compounds. more...

Organism:

Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL7137

2 Samples

Download data: PDF, TXT

(Submitter supplied) The function of the Staphylococcus aureus eukaryotic-like serine/threonine protein kinase PknB was investigated by transcriptome analysis using DNA-microarray technology and biochemical assays. The transcriptional profile reveals a strong regulatory impact of PknB on the expression of genes encoding proteins which are involved in purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, and glutamine synthesis.

Organism:

Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315

Type:

Expression profiling by array

Platform:

GPL5429

5 Samples

Download data: GPR

(Submitter supplied) Analysis of the intergenic regions of Staphylococcus aureus by bioinformatics predicted multiple regulatory regions. From this analysis, we also characterized 11 novel noncoding RNAs (RsaA-K) that are expressed in several S. aureus strains under different experimental conditions. Many of them accumulate in the late-exponential phase of growth. All ncRNAs are stable and their expression is Hfq-independent. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MSSA476

Type:

Non-coding RNA profiling by array

Platform:

GPL7137

2 Samples

Download data: TXT

(Submitter supplied) The genome sequence is the “blue-print of life”, but proteomics is required to relate this blue-print of life to the actual physiology of living cells. Because of their low complexity – only about 2.000 proteins make a Staphylococcus aureus cell viable – bacteria are excellent model systems to identify the entire protein assembly of a living organism. Many of the studies on physiological proteomics of bacteria, however, still rely on 2D gel-based technologies that visualize only a minor part of the proteome. more...

Organism:

Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300; Staphylococcus aureus subsp. aureus COL

Type:

Expression profiling by array

Platform:

GPL7137

4 Samples

Download data: TXT

(Submitter supplied) The lantibiotic mersacidin is an antimicrobial peptide of 20 amino acids that is produced by Bacillus sp. strain HIL Y-85,54728. Lantibiotics are antibiotics containing nonproteinogenic amino acids like lanthionine and/or 3-methyllanthionine. Mersacidin interferes with cell wall biosynthesis by targeting the peptidoglycan precursor lipid II and inhibits the growth of MRSA and other gram-positive bacteria. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus N315

Type:

Expression profiling by array

Platform:

GPL5421

2 Samples

Download data: GPR

(Submitter supplied) CodY is a conserved regulator in gram-positive organisms described to repress metabolic genes mainly involved in nitrogen metabolism but also to control the expression of virulence genes in pathogens. We constructed codY gene-replacement mutants in three unrelated S. aureus strains (Newman, UAMS-1, RN1HG). codY mutants grew slower in a chemically defined medium compared to the wild type strains. However, only codY mutants were able to grow in medium lacking threonine. more...

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus str. Newman; Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus USA300

Type:

Non-coding RNA profiling by array

Platform:

GPL7137

8 Samples

Download data

(Submitter supplied) The lantibiotic mersacidin is an antimicrobial peptide of 20 amino acids that is ribosomally produced by Bacillus sp. strain HIL Y-85,54728. Mersacidin acts by complexing the sugar phosphate head group of the peptidoglycan precursor lipid II, thereby inhibiting the transglycosylation reaction of peptidoglycan biosynthesis. Here, we studied the transcriptomic response of S. aureus to subinhibitory concentrations of mersacidin using microarray and qRT-PCR techniques. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus N315

Type:

Expression profiling by array

Platforms:

GPL5421 GPL5429

14 Samples

Download data: GPR

(Submitter supplied) The sciTRACER S. aureus contains 2338 PCR products from all 2593 ORFs in the annotated genome (Ref ID: NC_002745), arranged in unique and second sequences: since the ORFs sizes range from 51 bp (e.g.: 13700427) up to 2kb (e.g.: 13701232), additional second PCR products have been developed. As unique sequences, 2333 PCR products and as second sequences, 333 PCR products are immobilized from which 5 are not in the group of unique gene fragments. more...

Organism:

Staphylococcus aureus subsp. aureus N315

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(Submitter supplied) The sciTRACER S. aureus contains 2338 PCR products from all 2593 ORFs in the annotated genome (Ref ID: NC_002745), arranged in unique and second sequences: since the ORFs sizes range from 51 bp (e.g.: 13700427) up to 2kb (e.g.: 13701232), additional second PCR products have been developed. As unique sequences, 2333 PCR products and as second sequences, 333 PCR products are immobilized from which 5 are not in the group of unique gene fragments. more...

Organism:

Staphylococcus aureus subsp. aureus N315

1 Series

6 Samples

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(Submitter supplied) 60-mers oligonucleotide probes, in-situ synthesis Protocol: Sure Print Technology-In situ synthesis

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus NCTC 8325; Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus subsp. aureus Mu50; Staphylococcus aureus subsp. aureus N315; Staphylococcus aureus subsp. aureus MW2; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476; Staphylococcus aureus subsp. aureus USA300

11 Series

74 Samples

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(Submitter supplied) The sciTRACER S. aureus contains 2338 PCR products from all 2593 ORFs in the annotated genome (Ref ID: NC_002745), arranged in unique and second sequences: since the ORFs sizes range from 51 bp (e.g.: 13700427) up to 2kb (e.g.: 13701232), additional second PCR products have been developed. As unique sequences, 2333 PCR products and as second sequences, 333 PCR products are immobilized from which 5 are not in the group of unique gene fragments. more...

Organism:

Staphylococcus aureus subsp. aureus N315

2 Series

9 Samples

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