GEO DataSets

(Submitter supplied) We investigated root associated fungi in young Norway spruce (Picea abies) cuttings rooted from slow- and fast-growing trees showing variable growth rate in long-term field experiments and compared their roots’ gene expression patterns five and 18 months after adventitious root initiation. Gene expression patterns of adventitious roots could not be systematically linked with the growth phenotype at the initiation of root formation, and thus fundamental differences in the receptiveness of fungal symbionts could not be assumed.

Organism:

Picea glauca; Picea abies; Picea sitchensis

Type:

Expression profiling by array

Platform:

GPL15033

19 Samples

Download data: TXT

(Submitter supplied) Tree improvement programs select genotypes for faster growth, at both early and late stages, to increase yields over unimproved material, and the improvement is frequently attributed to genetic control in growth parameters among genotypes. Underutilized genetic variability among genotypes also has the potential to ensure future gains are possible. However, the genetic variation in growth, physiology and hormone control among genotypes generated from different breeding strategies has not been well characterized in conifers. more...

Organism:

Picea glauca

Type:

Expression profiling by RT-PCR

Platform:

GPL32694

69 Samples

Download data: XLSX

(Submitter supplied) Following previous work, we extend the use of a reliable detection method to a wide progeny in white spruce in order to genetically map a maximum of inherited gene CNVs and linked those to phenotypic traits related to adaptation. We selected probes targeting 3911 genes likely in CNV in this progeny and performed genetic mapping for those harboring a 1:1 segregation. In addition, the link between gene CNVs and phenotypic variation was tested using budburst, budset and growth data for the same samples. more...

Organism:

Picea glauca

Type:

Genome variation profiling by array

Platform:

GPL23818

115 Samples

Download data: TXT

(Submitter supplied) We developed a reliable CNV detection method for species lacking contiguous reference genome. We selected multiple probes within 14,078 gene sequences and developed comparative genome hybridization on arrays. Gene CNVs were assessed in three full-sib families from species with 20 Gb genomes, i.e. white and black spruce, and interior spruce - a natural hybrid. Results: We discovered hundreds of gene CNVs in each species, 3612 in total, which were enriched in functions related to stress and defense responses and narrow expression profiles, indicating a potential role in adaptation. more...

Organism:

Picea mariana; Picea glauca; Picea engelmannii x Picea glauca

Type:

Genome variation profiling by array

Platform:

GPL22783

73 Samples

Download data: TXT

(Submitter supplied) The goal is to identify tissue specific patterns of expression in Picea glauca seedlings

Organism:

Picea glauca; Picea sitchensis

Type:

Expression profiling by array

Platform:

GPL15033

56 Samples

Download data: TXT

(Submitter supplied) The goal is to look at changes in the pattern of expression of the xylem transcriptome through the growth season in two spruces (Picea glauca and Picea abies).

Organism:

Picea abies; Picea sitchensis; Picea glauca

Type:

Expression profiling by array

Platform:

GPL15033

48 Samples

Download data: TXT

(Submitter supplied) It is crucial for north temperate tree species like white spruce to control timing of growth cessation and bud set in order to survive winter conditions. While much work has examined both endogenous and exogenous mechanisms influencing this transition within angiosperms, the mechanisms underlying this transition in conifers; including photoperiod perception, growth cessation and development of endodormancy, have been less well-characterized. more...

Organism:

Picea glauca; Picea sitchensis

Type:

Expression profiling by array

Platform:

GPL15033

4 Samples

Download data: TXT

(Submitter supplied) We report the discovery of a beta-glucosidase gene (Pgβglu-1) whose expression underpins natural resistance to a major forest pest, the spruce budworm (SBW) in white spruce (Picea glauca (Voss.) Moench). We performed a microarray experiment to compare resistant (R) and non-resistant (N-R) trees. Pgβglu-1 transcripts levels uniquely were up to 1000 times higher in phenotypically resistant trees and correlated with accumulation of acetophenones compounds that reduce SBW development. more...

Organism:

Picea sitchensis; Picea glauca

Type:

Expression profiling by array

Platform:

GPL15033

42 Samples

Download data: TXT

(Submitter supplied) Canola (oilseed rape, Brassica napus L.), is susceptible to infection by the biotrophic protist Plasmodiophora brassicae, the causal agent of clubroot. To understand the roles of microRNAs (miRNAs) during the post-transcriptional regulation of disease initiation and progression, we have characterized the changes in miRNA expression profiles in canola roots during clubroot disease development and have compared these to uninfected roots. more...

Organism:

Physcomitrium patens; Persea americana; Populus trichocarpa x Populus deltoides; Malus domestica; Nicotiana sylvestris; Nicotiana tabacum; Petunia x hybrida; Oryza sativa; Lolium arundinaceum; Lotus japonicus; Cenchrus ciliaris; Populus tremula x Populus tremuloides; Nasturtium officinale; Picea glauca; Populus tremuloides; Populus trichocarpa; Prunus persica; Medicago truncatula; Nicotiana benthamiana; Hordeum vulgare; Ipomoea nil; Prunus armeniaca; Peridictyon sanctum; Oryza barthii; Hordeum vulgare subsp. vulgare; Oenocarpus bataua; Pinus taeda; Liriodendron tulipifera; Phaseolus vulgaris; Ipomoea batatas; Lactuca sativa; Oryza rufipogon; Oryza nivara; Sorghum bicolor; Rorippa indica; Prunus salicina; Populus tremula; Malcolmia maritima; Picea sitchensis; Brassica napus; Lupinus luteus; Phaseolus coccineus; Manihot esculenta; Ricinus communis; Oryza brachyantha; Cenchrus americanus; Phleum pratense; Panicum virgatum; Nuphar advena

Type:

Non-coding RNA profiling by array

Platform:

GPL17805

4 Samples

Download data: TXT

(Submitter supplied) The goal is to examine the molecular aspects underpinning somatic embryogenesis (SE) within shoot tissues of adult white spruce trees by conducting transcriptome-wide expression profiling of shoot explants taken from responsive and nonresponsive genotypes.

Organism:

Picea glauca; Picea sitchensis

Type:

Expression profiling by array

Platform:

GPL15033

20 Samples

Download data: TXT

(Submitter supplied) Heritable variation in gene expression has been largely studied in model organisms by taking advantage of pure and inbred lines. Comparative studies on organisms with contrasting evolutionary history, population size and genome architecture have been hindered by practical considerations, as many of these species are poorly suited for breeding. We have developed a method for determining expression variation in an undomesticated tree species by directly measuring the segregation of gene expression in the haploid meiotic products of a single diploid individual. more...

Organism:

Picea glauca; Picea sitchensis

Type:

Expression profiling by array

Platform:

GPL15033

72 Samples

Download data: TXT

(Submitter supplied) The goal of this experiment is to assess tissue preferential transcript accumulation and fold difference between two tissues that support secondary vascular growth in three spruces: Picea glauca, Picea sitchensis and Picea mariana. Tissues compared are secondary xylem (wood forming tissue located on the internal side of the cambial meristem) and phelloderm (composite sample of the phloem and phelloderm tissues located on the outer side of the cambial meristem).

Organism:

Picea glauca; Picea mariana; Picea sitchensis

Type:

Expression profiling by array

Platform:

GPL15033

100 Samples

Download data: TXT

(Submitter supplied) The goal of this experiment is to evaluate the potential for utilising this oligonucleotide microarray in other species and genera of the Pinaceae family by using comparative RNA hybridizations in four different spruces (Picea spp), two pines (Pinus spp.) and a larch (Larix laricina), across two tissues, xylem and phelloderm.

Organism:

Larix laricina; Picea glauca; Picea mariana; Picea sitchensis; Pinus strobus; Pinus resinosa; Picea abies

Type:

Expression profiling by array

Platform:

GPL15033

142 Samples

Download data: TXT

(Submitter supplied) A small database of transcription profiles called PgGenExpress was developed from over 150 hybridizations spanning eight major tissue types. In total, transcripts were detected for 92% of the genes on the microarray, in at least one tissue. Non-annotated genes were predominantly expressed at low levels in fewer tissues than genes of known or predicted function. Diversity of expression within gene families may be rapidly assessed from PgGenExpress. more...

Organism:

Picea sitchensis; Picea abies; Picea glauca

Type:

Expression profiling by array

Platform:

GPL15033

149 Samples

Download data: TXT

(Submitter supplied) In the autumn, stems of woody perennials such as forest trees undergo a transition from active growth to dormancy. We used microarray transcriptomic profiling in combination with a proteomics analysis to elucidate processes that occur during this growth-to-dormancy transition in a conifer, white spruce (Picea glauca [Moench] Voss). Several differentially expressed genes were likely associated with the developmental transition that occurs during growth cessation in the cambial zone and the concomitant completion of cell maturation in vascular tissues. more...

Organism:

Picea glauca

Type:

Expression profiling by array

Platform:

GPL10754

4 Samples

Download data: GPR

(Submitter supplied) Bud formation is an adaptive trait that temperate forest trees have acquired to facilitate seasonal synchronization. We have characterized transcriptome-level changes that occur during bud formation of white spruce (Picea glauca [Moench] Voss.), a primarily determinate species in which preformed stem units contained within the apical bud constitute most of next season's growth. Microarray analysis identified 4460 differentially expressed sequences in shoot tips during short day-induced bud formation. more...

Organism:

Picea glauca

Type:

Expression profiling by array

Platform:

GPL10754

26 Samples

Download data: TXT

(Submitter supplied) Diversification of defense-related compounds is a plant defense mechanism against the diverse array of natural enemies in the course of evolution, and is known to involve various sources of tissue. Here a large-scale survey of gene expression is conducted from three different sources of tissue; needles, phloem with the attached bark, and xylem, with an emphasis on the expression of the secondary metabolites of the core phenylpropanoid pathway and derivative pathways, taken together as phenolic defense-related compounds. more...

Organism:

Picea glauca; Picea mariana; Picea omorika; Picea jezoensis; Picea; Picea abies

Type:

Expression profiling by array

Platform:

GPL5423

45 Samples

Download data: TXT

(Submitter supplied) RT-PCR assay Protocol: see manufacturer's website

Organism:

Picea glauca

1 Series

69 Samples

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(Submitter supplied) Genotypic custom probes filtered from 4x180K CGH array. Protocol: See manufacturer's web site at http://www.agilent.com/

Organism:

Picea glauca

1 Series

1 Related Platform

115 Samples

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(Submitter supplied) Genotypic custom probes filtered from 4x180K CGH array. Arrays of this design have barcodes that begin with 16064728 or 2564728. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. The ID column represents the Agilent Feature Extraction feature number. more...

Organism:

Picea glauca

1 Related Platform

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