GEO DataSets

(Submitter supplied) The transcriptome of Blochmannia floridanus, the endosymbiont of the carpenter ant Camponotus floridanus, is presented during eight developmental stages of its holometabolous host by use of a whole genome DNA-macroarray. The detected transcription patterns indicate the presence of local transcription units as well as global regulatory mechanisms. Yet, the overall regulation scale is very modest, rarely exceeding a factor of three which is in line with the low number of transcriptional regulators present in this reduced genome. more...

Organism:

Candidatus Blochmanniella floridana

Type:

Expression profiling by array

Platform:

GPL7048

32 Samples

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(Submitter supplied) whole genome DNA macroarray Protocol: Gene-specific PCR primer pairs were designed and synthesized by MWG Biotech (Ebersberg, Germany) based on the Bl. floridanus genome sequence (RefSeq accession no NC_005061.1) to yield products of approximately 500 bp. We amplified 564 genes in duplicate 50 µl reactions, omitting the genes for most non-coding RNAs and several ribosomal subunit proteins. Reactions contained 1x PCR buffer with 1.5 mM MgCl2, 0.2 mM dNTPs, 0.8 µM each primer, 1.25 units Taq polymerase (Molzym, Bremen, Germany) and 4 ng of purified Bl. more...

Organism:

Candidatus Blochmanniella floridana

1 Series

32 Samples

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